Relationship between serum NDRG3 and papillary thyroid carcinoma

Abstract

BackgroundIn recent years, papillary thyroid carcinoma is considered to be one of the fastest increaseing cancer. NDRG family member 3 (NDRG3) has been proposed as a molecular marker of tumor, and is expected to be used in clinic.MethodsEnzyme-linked immunosorbent assay was used to detect the serum NDRG3 expression in 81 papillary thyroid carcinoma cases, 75 benign thyroid nodules cases and 77 healthy control cases, respectively. Electrochemiluminescence method was applied to measure the levels of triiodothyronine, tetraiodothyronine, thyrotropin, thyroglobulin antibody and thyroid peroxidase antibody. Immunohistochemical staining was used to detect the expression of NDRG3 in papillary thyroid carcinoma, benign thyroid nodules and normal tissues adjacent to cancer.ResultsThe expression of serum triiodothyronine, tetraiodothyronine, thyrotropin, thyroglobulin antibody and thyroid peroxidase antibody and NDRG3 were significantly different among benign thyroid nodules, papillary thyroid carcinoma cases and healthy control groups (P <0.001). Only the expression of serum NDRG3 was significantly different between benign thyroid nodules and papillary thyroid carcinoma groups (P <0.001). Immunohistochemistry showed that NDRG3 was expressed in all three groups, the lowest in papillary thyroid carcinoma, the second in benign thyroid nodules, and the highest in normal tissues adjacent to cancer. Logistic regression analysis showed that serum NDRG3 was an independent protective factor for papillary thyroid carcinoma (OR =0.964, 95%CI =0.953 to 0.974, P <0.001). The ROC curve of non-papillary thyroid carcinoma diagnosed by serum NDRG3 showed the optimal cut-off value of 481.38 pg/ml, sensitivity of 72.4%, specificity of 90.1%, and the maximum area under the curve (AUC =0.902, 95%CI =0.863 to 0.940, P <0.001). The ROC curve of benign thyroid nodules diagnosed by serum NDRG3 showed the optimal critical value of 459.28 pg/ml, sensitivity of 81.3%, and specificity of 74.1% (AUC =0.863, 95%CI =0.808 to 0.919, P <0.001). The expression level of serum NDRG3 was significantly correlated with extrathyroid extensionand (P =0.007) and lymphatic metastasis of papillary thyroid carcinoma (P =0.019).ConclusionsThe decrease of NDRG3 expression can not only differential diagnosis benign thyroid nodules and papillary thyroid carcinoma, but also serve as a molecular marker for the diagnosis of papillary thyroid carcinoma.

piRNAs and miRNAs Can Bind to mRNA of <i>KLOTHO</i> and <i>FGF23</i> Genes

“…The DAZAP1 gene is highly expressed in testis (RPKM 19.6), appendix (RPKM 11.8). A number of publications have shown the involvement of the NDRG3 gene in the development of oncogenesis, and in most cases, increased expression is observed in cancer of various organs [70][71][72][73][74][75][76]. Therefore, it is important to know whether piR-3215034 and piR-6885965 can suppress the expression of the NDRG3 gene.…”

Comprehensive profiling of endocrine metabolism identifies a novel signature with robust predictive value in ovarian cancer

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“…demonstrated that DNAJA1 expression in clinical tumor tissues was significantly higher than in normal tissues and that high DNAJA1 mRNA expression was associated with low survival in breast cancer. NDRG3 has been implicated in many types of cancer and is a member of the NDRG family 33–36 . NDRG3 expression was associated with various types of infiltrated immune cells 36 .…”

Effect of Air Pollutants and Meteorological Factors on Daily Outpatient Visits of Allergic Rhinitis in Hohhot, China

“…Viral infections inhibit the activity of ciliated cells and phagocytosis of alveolar macrophages, which in turn causes the invasion of bacteria, including Streptococcus pneumoniae and Klebsiella pneumoniae. 26 Meanwhile, persistent low temperatures may cause the combustion of petroleum products to release more toxic byproducts, such as NO 2. 27 These factors contribute to the frequency of respiratory illnesses under low-temperature conditions.…”