Relationship between serum leptin immunoreactivity and body fat mass as estimated by use of a novel gas-phase Fourier transform infrared spectroscopy deuterium dilution method in cats

Backus, Robert C.; Havel, Peter J.; Gingerich, Ronald L.; Rogers, Quinton R.

doi:10.2460/ajvr.2000.61.796

Cited by 80 publications

(99 citation statements)

References 30 publications

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“…In addition, we confirmed that anti-canine leptin antibody showed differences in immunoreactivity among other species as compared to the canine leptin [14]. Recently, Backus et al [1] reported serum leptin-like immunoreactiv-ity in cats was poorly measurable with a RIA based on antisera against mouse, rat, human, and primate leptins, suggesting the low immunoreactivity of these antisera to feline leptin. These facts indicate that species-specific leptin and/or anti-leptin antibody is required for better understanding the physiological and pathological functions of leptin in detail.…”

Section: Discussionsupporting

confidence: 63%

cDNA Cloning of Feline Leptin and Its mRNA Expression in Adipose Tissue.

Sasaki

Shibata

Honjoh

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. Leptin, the product of the obese (ob) gene, is an adipocyte-derived hormone involved in regulating food intake and energy expenditure in humans and rodents. To determine the primary structure of feline leptin, we cloned the feline leptin cDNA using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of complementary DNA (cDNA) ends (RACE) methods. The full-length feline leptin cDNA was 2935 bp with a 501 bp open reading frame encoding the precursor peptide of 167 amino acids including 21 residues of signal peptide. The sequence of a 146-amino acid mature leptin was 81.5-91.8% homologous to those of other species. RT-PCR analysis revealed that the leptin mRNA was expressed in adipose tissues and not detected in liver, heart, kidney, lung, pancreas, brain and skeletal muscle. These data show that feline leptin is highly homologous to leptins of other species, and expressed in adipose tissues in cats. KEY WORDS: adipose tissue, cDNA cloning, feline, leptin.

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Section: Discussionsupporting

confidence: 63%

cDNA Cloning of Feline Leptin and Its mRNA Expression in Adipose Tissue.

Sasaki

Shibata

Honjoh

et al. 2001

The Journal of Veterinary Medical Science

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“…Leptin concentrations are positively correlated with adipose tissue mass (Appleton et al, 2000;Backus et al, 2000;Belsito et al, 2009). Backus et al (2000) used linear regression and estimated that for every 1 g increase in BW, a 1.2 ng/ml increase in leptin occurred.…”

Section: High-protein Diet Following Ovariohysterectomymentioning

confidence: 99%

“…Backus et al (2000) used linear regression and estimated that for every 1 g increase in BW, a 1.2 ng/ml increase in leptin occurred. In the current study, circulating blood leptin concentrations not only increased over time in cats fed both diets, but were also greater in MP-fed cats.…”

Section: High-protein Diet Following Ovariohysterectomymentioning

confidence: 99%

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Ovariohysterectomy alters body composition and adipose and skeletal muscle gene expression in cats fed a high-protein or moderate-protein diet

Vester

Sutter

Keel

et al. 2009

Animal

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The objective of this study was to measure changes in body composition, physical activity and adipose and skeletal muscle gene expression of cats fed a high-protein (HP) diet or moderate-protein (MP) diet, following ovariohysterectomy. Eight cats were randomized onto HP or MP diets and were fed those diets for several months prior to baseline. All cats underwent an ovariohysterectomy at baseline (week 0) and were allowed ad libitum access to dietary treatments for 24 weeks. Food intake was measured daily, and BW and body condition score were measured weekly. Blood, adipose and skeletal muscle tissue samples were collected, physical activity was measured, and body composition was determined using DEXA (dual-energy X-ray absorptiometry) at weeks 0, 12 and 24. Caloric intake increased soon after ovariohysterectomy, resulting in increased ( P , 0.05) BW at weeks 12 and 24 compared to week 0. Body condition score and body fat percentage increased ( P , 0.05) over time. Blood glucose increased ( P , 0.05) linearly over time. Non-esterified fatty acids were decreased ( P , 0.05) at weeks 12 and 24 compared to week 0. Blood leptin increased ( P , 0.05) over time. Total physical activity decreased ( P , 0.05) from week 0 to weeks 12 and 24 in all cats. Adipose tissue mRNA abundance of adiponectin, hormone sensitive lipase, toll-like receptor-4, uncoupling protein-2 (UCP2) and vascular endothelial growth factor decreased ( P , 0.05) linearly over time, regardless of diet. Skeletal muscle mRNA abundance for glucose transporter-1, hormone sensitive lipase and UCP2 were decreased ( P , 0.05), regardless of dietary treatment. Our research noted metabolic changes following ovariohysterectomy that are in agreement with gene expression changes pertaining to lipid metabolism. Feeding cats ad libitum after ovariohysterectomy is inadvisable.

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“…Plasma concentrations of leptin are positively correlated with body fat content in various mammalian species including ruminants [3][4][5][6][7][8][9][10][11]. Therefore, circulating leptin is a suitable marker for adiposity in livestock animals and pets, and for assessment of long-term nutritional condition in wild animals.…”

Section: Introductionmentioning

confidence: 99%

Inverse regulation of leptin mRNA expression by short- and long-chain fatty acids in cultured bovine adipocytes

Soliman

Kimura

Ahmed

et al. 2007

Domestic Animal Endocrinology

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Leptin is an adipose tissue-derived cytokine plays key roles in the regulation of food intake and energy expenditure. However, regulatory mechanisms of leptin gene expression are not fully elucidated in ruminants that utilize short-chain fatty acids (SCFA), known as volatile fatty acids, as principal energy sources. In this study, we determined effects of SCFA and long-chain fatty acids (LCFA) on leptin expression in bovine adipocytes. Bovine stromal vascular cells isolated from subcutaneous adipose tissue of Holstein cows were cultured to confluence and treated sequentially with dexamethasone and isobutylmethylxanthine for 2 days and insulin and troglitazone for 12 days to achieve full differentiation to adipocytes. The cells started to accumulate lipids 4 days after the onset of treatment, with increased mRNAs expression of leptin, as well as aP2, adiponectin, and PPAR-γ  Removal of fetal calf serum and reduction of glucose in the culture medium of differentiated adipocytes decreased leptin mRNA expression.Subsequent addition of acetate, butyrate, or propionate dose-dependently restored and rather increased leptin expression, while addition of LCFA suppressed it. The stimulatory effect of acetate was abolished by prior treatment of the cells with pertussis toxin and by addition of LCFA. Furthermore, cows fasted for 48h and fed thereafter, elaborate reduced and increased plasma leptin levels, respectively. Thus, these results suggest that plasma leptin levels in cows are inversely controlled at transcription by VFA and LCFA, and that the effects of SCFA possibly act through a G protein-coupled receptor for SCFA.

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Relationship between serum leptin immunoreactivity and body fat mass as estimated by use of a novel gas-phase Fourier transform infrared spectroscopy deuterium dilution method in cats

Cited by 80 publications

References 30 publications

cDNA Cloning of Feline Leptin and Its mRNA Expression in Adipose Tissue.

cDNA Cloning of Feline Leptin and Its mRNA Expression in Adipose Tissue.

Ovariohysterectomy alters body composition and adipose and skeletal muscle gene expression in cats fed a high-protein or moderate-protein diet

Inverse regulation of leptin mRNA expression by short- and long-chain fatty acids in cultured bovine adipocytes

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