Relationship between seminal plasma arginase activity and semen quality in Saanen bucks

Abstract

This study was conducted to determine the relationship between seminal plasma arginase activity and spermatological parameters in bucks. In this study, 5 ejaculates were collected by artificial vagina from each of 5 Saanen bucks of proven fertility. Spermatological parameters (semen volume, semen pH, mass sperm activity, sperm motility and concentration and percentage abnormal sperm) were evaluated immediately after collection in each ejaculate. After semen collection, samples were centrifuged and stored at −20 • C for analysis of the arginase activity. The mean level seminal plasma arginase activity recorded was 0.87 ± 0.12 units/mg protein. There existed a positive correlation between the seminal plasma arginase activity and sperm mass activity (r = 0.548, p < 0.01), sperm motility (r = 0.408, p < 0.05) and sperm concentration (r = 0.793, p < 0.01); However, a negative correlation was recorded between seminal plasma arginase activity and the percentage abnormal sperm (r = −0.427, p < 0.05). This study suggests that a significant correlation exists between seminal plasma arginase activity and certain spermatological parameters. Therefore, seminal plasma arginase activity may be used as a biochemical criterion to determine sperm quality in bucks.

Influence of vitamin E and vitamin E‐selenium combination on arginase activity, nitric oxide level and some spermatological properties in ram semen

“…However, there was an increase in seminal plasma arginase activity in vitamin E + selenium injected group, except for 72nd hr, but this increase was not statistically significant. Although some relationships between spermatological parameters and seminal plasma arginase activity have been reported (Gür & Kandemir, 2012;Türk, Gür, Kandemir, & Sönmez, 2011), no evidence was found effect of vitamin E and/or vitamin E + selenium combination on seminal plasma arginase activity.…”

“…However, there was an increase in seminal plasma arginase activity in vitamin E + selenium injected group, except for 72nd hr, but this increase was not statistically significant. Although some relationships between spermatological parameters and seminal plasma arginase activity have been reported (Gür & Kandemir, ; Türk, Gür, Kandemir, & Sönmez, ), no evidence was found effect of vitamin E and/or vitamin E + selenium combination on seminal plasma arginase activity. However, in a study conducted by adding vitamin E and selenium to diet, a significant increase in cardiac and renal arginase activity and a decrease hepatic arginase activity was observed in vitamin E ingested group, but no difference was found in the vitamin E + selenium combination group compared to the control group (Erişir, Beytut, Ozan, & Aksakal, ).…”

The kinetic properties of arginase in sperm cells of inferile men

“…It was found that plasma arginase activity was significantly lower than in the non-stress situation and during stress there was a negative correlation between the percentage of rapid progressive motility and arginase activity (Eskiocak et al, 2006). Similar relationships between the plasma arginase activity and the sperm concentration and sperm motility were detected in sheep and bulls (Gür et al, 2012;Türk et al, 2011). Although arginase activity has usually been detected in the reproductive tract, including spermatozoa, no data relating to the kinetic properties of enzyme in ejaculated spermatozoa has been reported.…”

Effect of sunflower lecithin on Kalahari Red goat semen during cryopreservation

“…The role of arginase in reproduction and fertility has been reported upon. Arginase enzyme has been found in ram epididymal spermatozoa (Mendez and Martinez, 1995), prostate and other parts of the reproductive system of ram (Razmi et al, 2004), prostate, seminal plasma, testis, human sperm cells (Keskinege et al, 2001) and in different parts of the reproductive system of bulls (Razmi et al, 2005) and in the seminal plasma of goat (Turk et al, 2011). The mean seminal arginase activity recorded in all the experiment in this study for both control and experimental group were lower than that in Saneen buck (Turk et al, 2011), WAD buck supplemented with vitamin E (Daramola et al, 2016) but higher than in cat (Mahmoud et al, 2007), normal men and men of oligospermia (Elgun et al, 2000).…”

“…Arginase enzyme has been found in ram epididymal spermatozoa (Mendez and Martinez, 1995), prostate and other parts of the reproductive system of ram (Razmi et al, 2004), prostate, seminal plasma, testis, human sperm cells (Keskinege et al, 2001) and in different parts of the reproductive system of bulls (Razmi et al, 2005) and in the seminal plasma of goat (Turk et al, 2011). The mean seminal arginase activity recorded in all the experiment in this study for both control and experimental group were lower than that in Saneen buck (Turk et al, 2011), WAD buck supplemented with vitamin E (Daramola et al, 2016) but higher than in cat (Mahmoud et al, 2007), normal men and men of oligospermia (Elgun et al, 2000). Previous studies had reported a positive correlation between seminal arginase activity and sperm motility (Elgun et al, 2000;Eskiocak et al, 2006;Turk et al 2011), which was also confirmed in this study except in experiment 1 where 1.5% sunflower lecithin extender recorded the significantly (P < 0.05) lowest seminal arginase activity.…”