Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay

Novotný, Jiřı́; Aziz, Nabil; Rybar, R.; Březinová, Jana; Kopecka, Vera; Filipčíková, Radka; Řeřuchová, Marie; Oborná, I

doi:10.5507/bp.2013.065

Cited by 21 publications

(19 citation statements)

References 26 publications

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“…Patients with oligozoospermia also displayed elevated SDF, in agreement with previous studies reporting significant negative correlations between sperm DNA damage and abnormal semen parameters, including low concentration (Fei et al, 2013;Novotny et al, 2013). The fact that patients with oligozoospermia frequently display reduced STL, high SDF and elevated aneuploidy rates suggests that diminished sperm concentration is symptomatic of meiotic and spermatogenic processes that are compromised on multiple levels.…”

Section: Article In Presssupporting

confidence: 85%

Investigation of sperm telomere length as a potential marker of paternal genome integrity and semen quality

Cariati

Jaroudi

Alfarawati

et al. 2016

Reproductive BioMedicine Online

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Recent studies have reported shorter sperm telomere length (STL) in men with idiopathic infertility. The aim of this study was to measure STL in semen samples from men to evaluate whether STL variation is associated with chromosomal abnormality, DNA fragmentation, traditional semen parameters, IVF outcome, or all four factors. A significant correlation between telomere length and diploidy was observed (P = 0.037). Additionally, STL was found to be positively associated with sperm count (P = 0.006); oligospermic samples had particularly short telomeres (0.9 ± 0.1 versus 1.4 ± 0.1; P = 0.0019). The results confirmed a link between sperm DNA fragmentation and aneuploidy, previously proposed (P = 0.009). A negative relationship was demonstrated between sperm concentration and aneuploidy and Sperm DNA framentation (P = 0.03, P < 0.0001, respectively). For a subset of 51 of the 73 sperm samples used for fertilization, IVF outcomes were known. A total of 17.6% of these samples had atypical STLs. None of these samples produced an ongoing pregnancy. In contrast, the pregnancy rate for samples that had STLs in the normal range was 35.7% (P = 0.044). In conclusion, STL has potential as a fast and inexpensive form of sperm quality assessment.

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Section: Article In Presssupporting

confidence: 85%

Investigation of sperm telomere length as a potential marker of paternal genome integrity and semen quality

Cariati

Jaroudi

Alfarawati

et al. 2016

Reproductive BioMedicine Online

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“…The flow cytometry is used in the SCSA to measure stability of double-stranded sperm chromatin and evaluation of results without manual operation. 47 In the current study, there were 93.3% (27 of 30) and 76.7% (23 of 30) at 1 month SH treatment phase with a DFI greater than 20% and greater than 30%, respectively. In the current study, we found that subjects of SH treatment phase have a significantly higher percentage of sperm with fragmented DNA by using SCSA compared with recovery time.…”

Section: Discussionsupporting

confidence: 43%

Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men

Zhang

Zhai

Fang

et al. 2018

J of Cellular Biochemistry

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At present, male contraceptive methods are only vasectomy and condoms, so it is necessary to research on male contraceptive techniques. The aim of this study is to observe the effects of scrotal heating (SH) on semen parameters, seminal l-carnitine (LC), epidermal growth factor (EGF), macrophage migration inhibitory factor (MIF), reproductive hormones and sperm chromosome numbers of adult healthy men, and to provide the experimental data for male contraception. The scrotums of 30 healthy male volunteers were exposed to the condition of 40 to 43°C SH belt warming 40 minutes each day for successive 2 days per week. The course of SH was continuous for 3 months. Computer-assisted semen analysis and hypo-osmotic swelling test, sperm DNA integrity, l-carnitine, MIF and EGF, and sperm fluorescence in situ hybridization were performed before, during, and after SH. The serum level of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) were measured by chemiluminescent immunoassay. The mean parameters of sperm concentration, vitality, and normal morphological sperm were significantly decreased in groups with sperms being collected during 1, 2, and 3 months of SH when compared with those in groups of pre-SH (P < 0.01). Statistically significant differences of sperm DNA fragmentation, normal sperm membrane functionality, levels of LC and MIF in semen, and LH, FSH, and T in serum were observed between the groups of before SH and after SH 3 months and the groups of during SH 1, 2, and 3 months (P < 0.001). The total rate of chromosome number for 13, 18, 21, X, and Y in the 3 months of SH was 13.7-fold greater (13.72%/1.69%) than before SH (P < 0.001). The constant SH can impact the semen quality, sperm DNA integrity, sperm chromosome, LC and MIF, and LH, FSH, and T in serum. Transient SH may be a new method for male contraception.

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“…High ROS concentrations in infertile men have been associated with DNA fragmentation and poor chromatin packing [Kodama et al 1997;Aitken and Krausz, 2001;Aitken et al 2003;Moustafa et al 2004;Cocuzza et al 2007;Desai et al 2009;Aitken and De Iuliis, 2010;Novotny et al 2013].…”

Section: Oxidative Stressmentioning

confidence: 99%

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

Agarwal

Roychoudhury

Bjugstad³

et al. 2016

Therapeutic Advances in Urology

138

118

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The diagnosis of male infertility relies largely on conventional semen analysis, and its interpretation has a profound influence on subsequent management of patients. Despite poor correlation between conventional semen parameters and male fertility potential, inclusion of advanced semen quality tests to routine male infertility workup algorithms has not been widely accepted. Oxidative stress is one of the major mediators in various etiologies of male infertility; it has deleterious effects on spermatozoa, including DNA damage. Alleviation of oxidative stress constitutes a potential treatment strategy for male infertility. Measurement of seminal oxidative stress is of crucial role in the identification and monitoring of patients who may benefit from treatments. Various tests including reactive oxygen species (ROS) assay, total antioxidant capacity (TAC) assay or malondialdehyde (MDA) assay used by different laboratories have their own drawbacks. Oxidation-reduction potential (ORP) is a measure of overall balance between oxidants and antioxidants, providing a comprehensive measure of oxidative stress. The MiOXSYS™ System is a novel technology based on a galvanostatic measure of electrons; it presents static ORP (sORP) measures with static referring to the passive or current state of activity between oxidants and antioxidants. Preliminary studies have correlated sORP to poor semen qualities. It is potentially useful in prognostication of assisted reproductive techniques outcomes, screening of antioxidants either in vivo or during IVF cycles, identification of infertile men who may benefit from treatment of oxidative stress, and monitoring of treatment success. The simplified laboratory test requiring a small amount of semen would facilitate clinical application and research in the field. In this paper, we discuss the measurement of ORP by the MiOXSYS System as a real-time assessment of seminal oxidative stress, and argue that it is a potential valuable clinical test that should be incorporated into the male infertility workup and become an important guide to the treatment of oxidative stress-induced male infertility.

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Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay

Cited by 21 publications

References 26 publications

Investigation of sperm telomere length as a potential marker of paternal genome integrity and semen quality

Investigation of sperm telomere length as a potential marker of paternal genome integrity and semen quality

Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

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