Relationship between pyrethroid enhanced batrachotoxinin A 20-α-benzoate binding and pyrethroid toxicity to susceptible and resistant tobacco budworm moths Heliothis virescens

Church, Cole J.; Knowles, Charles O.

doi:10.1016/0742-8413(93)90036-k

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…o i r e s c e n~~~ and that a decreased affinity of the pyrethroid binding site in the sodium channel protein is at least partly responsible for nerve insensitivity in these insects. 36 In the absence of definitive data, the neurophysiological assay constructed here attempts to distinguish nerve-sensitive individuals from those expressing nerve insensitivity to pyrethroids.…”

Section: Discussionmentioning

confidence: 99%

Nerve insensitivity resistance to cypermethrin in larvae of the tobacco budworm Heliothis virescens from USA cotton field populations

1995

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Spontaneous neuronal activity was recorded from the peripheral nerves of third-instar larvae of strains of Heliothis uirescens (F.) obtained directly from cotton fields in the USA. Following a control period the preparations were exposed to increasing concentrations of cis-cypermethrin in a cumulative doseresponse assay. A positive response was defined as an increase of at least five-fold in the rate of neuronal activity over that seen during the control period. Up to 35 individuals of each strain were assayed and the responses used to construct a phenotypic profile categorising the individuals from nerve-susceptible to highly nerve-insensitive. An EC,, for the action of cis-cypermethrin was also obtained. There was a positive, significant correlation between non-synergisable resistance to cypermethrin and nerve insensitivity as defined in the neurophysiological assay. It was shown that nerve insensitivity to cypermethrin increased throughout the cotton-growing season.

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Section: Discussionmentioning

confidence: 99%

Nerve insensitivity resistance to cypermethrin in larvae of the tobacco budworm Heliothis virescens from USA cotton field populations

1995

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“…The presence of receptor sites 1 to 4 has been indicated by the binding of: 3H-saxitoxin and TTX (receptor site 1, Gordon et al, 1985); tritiated derivative of batrachotoxin and veratridine (receptor site 2, Soderlund et al, 1987;Dong et al, 1993;Church and Knowles, 1993); 125I-a-scorpion (LqhaIT) and 125I-ATX II sea anemone toxins (receptor site 3, Gordon and Zlotkin, 1993;Pauron et al, I985; and 125I-{3-scorpion toxins (Ts VII, Css VI, receptor site 4, Lima et al, 1986Lima et al, , 1989, on locust, cockroach and other insect neuronal membranes. The presence of receptor site 5 on insect sodium channels has been demonstrated by the effect of brevetoxin on a cockroach axon and by the allosteric enhancement of scorpion a-toxin binding (LqhalT) on locust sodium channels (Cest~le et al, 1995;Gordon et al, 1996).…”

Section: G O R D O Nmentioning

confidence: 99%

A new approach to insect-pest control—combination of neurotoxins interacting with voltage sensitive sodium channels to increase selectivity and specificity

Gordon

1997

Invertebrate Neuroscience

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Voltage-sensitive sodium channels are responsible for the generation of electrical signals in most excitable tissues and serve as specific targets for many neurotoxins. At least seven distinct classes of neurotoxins have been designated on the basis of physiological activity and competitive binding studies. Although the characterization of the neurotoxin receptor sites was predominantly performed using vertebrate excitable preparations, insect neuronal membranes were shown to possess similar receptor sites. We have demonstrated that the two mutually competing anti-insect excitatory and depressant scorpion toxins, previously suggested to occupy the same receptor site, bind to two distinct receptors on insect sodium channels. The latter provides a new approach to their combined use in insect control strategy. Although the sodium channel receptor sites are topologically separated, there are strong allosteric interactions among them. We have shown that the lipid-soluble sodium channel activators, veratridine and brevetoxin, reveal divergent allosteric modulation on scorpion alpha-toxins binding at homologous receptor sites on mammalian and insect sodium channels. The differences suggest a functionally important structural distinction between these channel subtypes. The differential allosteric modulation may provide a new approach to increase selective activity of pesticides on target organisms by simultaneous application of allosterically interacting drugs, designed on the basis of the selective toxins. Thus, a comparative study of neurotoxin receptor sites on mammalian and invertebrate sodium channels may elucidate the structural features involved in the binding and activity of the various neurotoxins, and may offer new targets and approaches to the development of highly selective pesticides.

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Resistance to insecticides in Heliothine Lepidoptera: a global view

McCaffery

1998

Phil. Trans. R. Soc. Lond. B

195

123

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The status of resistance to organophosphate, carbamate, cyclodiene and pyrethroid insecticides in the heliothine Lepidoptera is reviewed. In particular, resistance in the tobacco budworm, Heliothis virescens, and the corn earworm, Helicoverpa zea, from the New World, and the cotton bollworm, Helicoverpa armigera, from the Old World, are considered in detail. Particular emphasis has been placed on resistance to the most widely used of these insecticide groups, the pyrethroids. In each case, the incidence and current status of resistance are considered before a detailed view of the mechanisms of resistance is given. Controversial issues regarding the nature of mechanisms of resistance to pyrethroid insecticides are discussed. The implications for resistance management are considered.

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Relationship between pyrethroid enhanced batrachotoxinin A 20-α-benzoate binding and pyrethroid toxicity to susceptible and resistant tobacco budworm moths Heliothis virescens

Cited by 3 publications

References 22 publications

Nerve insensitivity resistance to cypermethrin in larvae of the tobacco budworm Heliothis virescens from USA cotton field populations

Nerve insensitivity resistance to cypermethrin in larvae of the tobacco budworm Heliothis virescens from USA cotton field populations

A new approach to insect-pest control—combination of neurotoxins interacting with voltage sensitive sodium channels to increase selectivity and specificity

Resistance to insecticides in Heliothine Lepidoptera: a global view

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