Relationship between polymorphisms in exon 10 of FSHR gene and litter size in swine

Abstract

ABSTRACT. Follicle-stimulating hormone (FSH), a glycoprotein secreted by the anterior pituitary, can regulate ovarian function through the FSH receptor (FSHR). To evaluate the effects of the FSHR gene on reproductive traits in pigs, polymorphisms in exon 10 of the FSHR gene were observed by polymerase chain reaction-single-strand conformation polymorphism, and 3 single nucleotide polymorphisms (C1491T, G1885A, and C1977T) in exon 10 of the porcine FSHR gene, and 3 genotypes (AA, AB, and BB) for C1491T and 2 haplotypes (D and E) for G1885A and C1977T were identified. Further analysis of single nucleotide polymorphism genotypes associated with reproductive traits including total number born (TNB) and number born alive (NBA) was carried out in 3 pig populations including Berkshire, Wannan Black (a Chinese indigenous pig breed), and BW pigs (two-way crossbred pigs produced from Berkshire ♂ and Wannan Black pig ♀). The results showed that the TNB and NBA of Wannan Black pigs with the AB genotype were significantly higher than in AA genotype sows (P < 0.01) 8253 FSHR gene association with litter size in swine ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8252-8261 (2015) in multiparity sows and all parities. The TNB and NBA of Berkshire pigs with the DE genotype were significantly higher than the DD and EE genotype sows (P < 0.01) in gilts, sows and all parities. Overall, TNB and NBA from the 3 identified genotypes was DE > DD > EE. The results showed that polymorphisms in exon 10 of the FSHR gene had a significant effect on litter size traits of Wannan Black and Berkshire pigs. These results can be applied for marker-assisted selection in the 2 swine breeds.

Genome-wide detection of genetic structure and runs of homozygosity analysis in Anhui indigenous and Western commercial pig breeds using PorcineSNP80k data

“…The Anhui Province is one of the top ten provinces that traditionally produce pigs in China, and it has abundant genetic resources of indigenous pig breeds (YZ, WNHZ, HZ, WNHUAZ, and LB). Due to long-term natural adaptation and artificial selection, the AHIPs have gradually evolved high fertility, high fat content, excellent meat quality [ 23 ], disease resistance [ 24 ], good maternal stability [ 25 ], and crude feed tolerance [ 22 ]. In this study, WECPs and AHIPs significantly differed in terms of genetic backgrounds, consistent with previous studies showing that pigs were domesticated in at least two separate domestication centres, Europe and Asia [ 26 – 28 ].…”

“…Although ROH has been used for breeding estimates in many Western commercial and Chinese indigenous pig breeds, it has been used less frequently in Anhui indigenous pig breeds (AHIPs), including the Wei (YZ), Wannan black (WNHZ), Huai (HZ), Wannanhua (WNHUAZ), and Six White (LB). These breeds have improved meat quality [ 23 ], disease resistance [ 24 ], and high fertility [ 25 ] compared with major commercial lean pig breeds. Nevertheless, the number of AHIPs has declined sharply in the past 20 years due to the large number of Western pig breeds that have been imported to improve leanness in pork (China National Commission of Animal Genetic Resources, 2011).…”

Population Structure and Selection Signatures Underlying Domestication Inferred from Genome-Wide Copy Number Variations in Chinese Indigenous Pigs

“…As a small indigenous population, WBP are at risk of extinction. In our previous studies, reproduction-, lipid-, meat-, and immune-related genes were identified in WBP, which could explain a part of the characteristics of WBP [ 19 , 20 , 21 , 22 , 23 ]. However, the above studies are all focused on the effect of SNP in revealing the genetic mechanism of characteristics in WBP.…”