Relationship between platelet volume and anti‐platelet autoantibodies in idiopathic thrombocytopenic purpura

Abstract

We used flow cytometry to explore the relationship between platelet volume and anti‐platelet autoantibodies in 71 patients with idiopathic thrombocytopenic purpura (ITP). An increase in platelet volume was found more frequently in patients with a platelet count of less than 20000/μl. Platelet volume was larger in patients without anti‐GPIIb/IIIa autoantibodies than in patients with these autoantibodies. Furthermore, the platelet count was significantly lower in patients without anti‐GIIb/IIIa autoantibodies than in the patients with these autoantibodies. There was a positive correlation between a large platelet volume in patients with a platelet count of less than 30000/μl and high platelet‐associated IgM levels. These results suggest that the platelet volume is related to the severity of thrombocytopenia in ITP.

Platelet apoptosis in paediatric immune thrombocytopenia is ameliorated by intravenous immunoglobulin

“…In keeping with previous observations (Kokawa et al , 1991; Saxon et al , 1998; Thomas‐Kaskel et al , 2007; Strauss et al , 2010), the median proportion of young, reticulated platelets as well as median platelet size was significantly elevated (median 14·9%; range 2·4–27·8; and median 32·8 FSC; range 13–109; n = 20) in our ITP study cohort, compared to that in healthy children (1·1%; range 0·6–3·0; and 19·0 FSC; range 13·6–24·1; n = 19) and cTP (1·8%; range 1·1–4·2; and 21·4 FSC; range 14·0–33·1; n = 8). There was no correlation between reticulated platelets and caspase‐3 activity ( r = 0·21, P = 0·4).…”

Mean platelet size related to glycoprotein‐specific autoantibodies and platelet‐associated IgG

“…Until now, relatively little attention has been paid to evaluating of platelet size in association with platelet antibody studies (Holme et al. , 1988b; Kokawa, Nomura & Yasunaga, 1991). In this study, platelet size (FSC) as well as PA‐IgG were statistically higher in patients than in healthy controls on the analysis of PA‐IgG.…”

“…In the present study, the frequency of GP IIbIIIa and/or IbIX, and/or GP V‐specific PA‐IgG was statistically higher in patients with larger platelets although the platelets were still within the reference range (FSC). Interestingly, Kokawa, Nomura & Yasunaga (1991) reported larger platelet volume in ITP patients without GP IIbIIIa‐specific antibodies than in those with GP IIbIIIa‐specific antibodies. Their separate analyses of GP IIbIIIa and IbIX specific antibodies may explain the disparity in results.…”

“…, 1988a; Holme et al. , 1988b; Kokawa, Nomura & Yasunaga, 1991; Ault & Mitchell, 1994; Leytin et al. , 1996; Hagenstrom et al.…”

Autoimmune Thrombocytopenia

“…Nevertheless, the detection of direct platelet eluates requires a selected patient population with a relatively high platelet count. Immunobead assay Disease-related TP 19 9 (47) 2 (11) Berchtold et al, 1989c Immunoprecipitation ITP 6 4 Tomiyama et al, 1990 Immunobead assay ITP children ITP adults 41 16 40 (98) 14 (88) 1 (2) 2 (13) Imbach et al, 1991 MAIPA Immune TP 29 21 (72) 22 (28) 14 (48) Kiefel et al, 1991 ELISA ITP 71 25 (35) 10 (14) Kokawa et al, 1991 Immunobead assay ITP In remission 53 17…”