Relationship between patterns of late S DNA synthesis and C- and G-banding in muntjac chromosomes

Expression of heterochromatin by restriction endonuclease treatment and distamycin A/DAPI staining of Indian muntjac (<i>Muntiacus muntjak</i>) chromosomes

“…The C-band pat tern is the same as the one described earlier (Comings. 1971: Sharma andDhaliwal. 1974).…”

Mechanisms of Chromosome Banding and Implications for Chromosome Structure

“…After chromosome banding showed that these regions had major G-bands while chromosome regions rich in R-bands contained early replicating DNA, it implied that G-bands were composed of AT-rich, late replicating DNA and R-bands were composed of GC-rich, early replicating DNA (143). Combi nations of autoradiography and banding studies show that R-bands are early replicating and G-bands are late replicating within the limits of resolu tion obtainable with autoradiography (144)(145)(146)(147)(148). The demonstration by Latt (149) that BrdU-containing DNA quenched the fluorescence of Ho echst 33258 (or acridine orange), allowed much higher resolution for study ing the relationship between the time of replication and G-or R-bands.…”

Similarity in the G-band patterns of constitutive heterochromatin of the composite X and Y chromosomes of certain rodents

“…Chromosomes were prepared from colchicinised bone marrow (in vivo) or PHA-stimulated spleen lymphocytes (in vitro). Techniques employed for chromosome preparations and C-and G-banding were the same as routinely used in this laboratory (Sharma & Dhaliwal, 1974). At least 4 individuals from each species were studied and a minimum of 5 G-banded plates were karyotyped from each individual…”