Relationship between outer membrane protein profiles and resistance to ceftazidime, imipenem, and ciprofloxacin inPseudomonas aeruginosa isolates from bacteremic patients

Abstract

Outer membrane protein (OMP) profiles of 122 Pseudomonas aeruginosa isolates recovered from the blood of bacteremic patients were analyzed to relate alterations in the expression of OMPs with porin activity to resistance to imipenem, ceftazidime, and ciprofloxacin. Imipenem-resistant isolates lacked or expressed reduced amounts of porin OprD. In contrast, alterations of OMP profiles were absent in most ceftazidime-resistant isolates. Six of 12 ciprofloxacin-resistant isolates had normal OMP profiles. The remaining isolates showed alterations in the expression of either OprC, OprF, or OprD. In addition, imipenem- and ceftazidime-resistant isolates displayed a beta-lactamase activity compatible with that of a group 1 chromosomal cephalosporinase.

Cellular impermeability and uptake of biocides and antibiotics in Gram-negative bacteria

“…In Ps. aeruginosa, antibiotic resistance has been associated with a deficient OprF and OprD (Buscher et al 1987;Godfrey and Bryan 1987;Piddock et al 1987;Quinn et al 1988;Gimeno et al 1996;Dubois et al 2001). In Enterobacter aerogenes, the emergence of multi-drug resistance has been associated with a decrease in outer membrane permeability associated with changes in porins (Charrel et al 1996;Chevalier et al 1999;De et al 2001), although the alteration in porins might be reversible when the selective pressure is removed (Bornet et al 2000).…”

Antimicrobial Susceptibility and Characterization of Outer Membrane Proteins of Aeromonas hydrophila Isolated in China

“…Up to now, the underlying outer membrane of A. hydrophila has not been studied extensively, especially, little is known about OMP profiles and their relationship with drug resistance, although such studies have been performed on A. salmonicida (Barnes et al 1990), Pseudomonas aeruginosa (Gimeno et al 1996), Haemophilus influenzae (Dixon et al 2008), Klebsiella pneumoniae (Hernandez-Alles et al 2000). The objectives of the present study, therefore, were to examine and compare the OMP profiles of 43 A. hydrophila isolates from diseased aquatic animals and revealed the relationship between OMP expression patterns and antimicrobial resistance.…”

Influencia del tabaquismo en la resistencia bacteriana después de la profilaxis frente a infecciones urinarias recurrentes con antibiótico o con vacuna

“…Pseudomona aeruginosa: a excepción de ciprofloxacino que mantiene sorprendentemente casi la misma tasa de resistencia inicial y fosfomicina que después de una aumento durante 8 años ha vuelto en los dos últimos a la tasa inicial, el resto con mayor o menor intensidad muestra una tendencia progresiva al alza, preocupante para gentamicina (8 vs. 38%), moderada para piperacilina (<1 vs. 20%) y tolerable para monobactams/carbapenems (1 vs. 8/10%, respectivamente) [59]. Parece que el mecanismo de acción de casi todas las cepas de Pseudomona aeruginosa resistentes a los carbapenems se debe a la presencia de metalo-beta-lactamasas y a alteraciones porínicas de la membrana (defecto de Opr-D), mientras que no se detecta tal situación en las cepas resistentes a cefatzidima y sólo en un 50% de las cepas resistentes a quinolonas [59,273,274].…”