Relationship between oocyte apoptosis and ovarian tumours induced by high and low LET radiations in mice

Nitta, Yumiko; Hoshi, Masaharu

doi:10.1080/0955300031000096315

Cited by 18 publications

(17 citation statements)

References 42 publications

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“…The expression of the Gcg gene in a-cells of the pancreatic islets was assessed immunohistochemically by the standard avidin-biotin complex method [15]. A polyclonal antibody to Gcg (CHEMICON Ltd., CA, USA) was applied as the first antibody at a titer of 1:500 on the paraffin section.…”

Section: Histopathological Analysismentioning

confidence: 99%

Kinetics of Blood Glucose in Mice Carrying Hemizygous Pax6

et al. 2009

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Abstract:The genotype-phenotype relationship was examined experimentally for the Pax6 Sey-4H mutant, which carries deletion of its chromosome 2 middle region hemizygously.The genotyping has indicated that this deleted segment is between 102.6 and 109.2 Mb from the centromere. The glucose-6-phosphatase gene followed by the glucagon and carboxyl ester lipase genes were mapped adjacent to the deleted region. Phenotyping indicates that the Pax6 Sey-4H mutant is more susceptible to diabetes. The glucose tolerance test showed that the mutants were less capable of reducing their level of blood glucose to the standard level than the normal sibs. The insulin-loading test revealed their inability to elevate their blood glucose levels up to normal levels. The time it took for the onset of diabetes induced by streptozotocin was shorter in the mutants than in normal sibs. Both the haploinsufficiency of the genes in the hemizygous segment of chromosome 2 and the quantitative imbalance of the whole genome could contribute the development of this phenotype in the mutant.

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Section: Histopathological Analysismentioning

confidence: 99%

Kinetics of Blood Glucose in Mice Carrying Hemizygous Pax6

et al. 2009

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“…The age of 10 weeks is best for irradiation to induce myeloid leukemia but not for the induction of solid tumors including intestinal tumors [5][6][7][8]11]. As the commonly deleted region of the two mutants was 3.2 Mb in length, the hemizygosity of this 3.2Mb segment of the chromosome 2 must have contributed to the development of the tumors not only by the half dose of the deleted genes but also through the mechanism of genomic instability.…”

Section: Discussionmentioning

confidence: 99%

“…The mice were exposed to whole body gamma-irradiation emitted from a 60 Co source (Shimadzu, Japan) was performed to the whole body at the age of 10 weeks in a single exposure with a dose of 3.0 Gy and a dose rate of 68.7 cGy/min [5][6][7]. The mice were observed for tumorigenicity until 18 months of age.…”

Section: Tumorigenesis Testmentioning

confidence: 99%

Intestinal Tumorigenicity of Mice Carrying Hemizygous Pax6, Pax6Sey-4H

Nitta¹,

Yoshida

Nakagata

et al. 2007

Exp. Anim.

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“…Sey3H hemizygous mutant were purchased from MRC (Harwell, OXON, UK), and the mutant has been maintained by crossing with C3H/He (Charles River Japan Inc.) 13 . Offspring were used for the following two experiments.…”

Section: Frozen Embryos Of the Pax6mentioning

confidence: 99%

“…A standard avidin-biotin complex technique 13 was performed to detect the peroxidase in the hematopoietic tumor cells in the liver. Polyclonal antibodies to peroxidase (MBL Inc., Japan) were used as the primary antibody at a titer of 1:1000.…”

Section: Histopathological Analysismentioning

confidence: 99%