Relationship between nuclear DNA fragmentation, mitochondrial DNA damage and standard sperm parameters in spermatozoa of infertile patients with leukocytospermia

Derbel, Rihab; Sellami, Hanen; Sakka, Rim; Slima, Ahlem Ben; Mkaddem, Ilyess; Gdoura, Radhouane; Mcelreavey, Elreavy; Keskes, Leila

doi:10.1016/j.jogoh.2021.102101

Cited by 16 publications

(12 citation statements)

References 75 publications

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“…The measurement of pro-inflammatory cytokines may be a convenient solution to this problem, but again, there is very little agreement on this issue. Interleukin-8 (IL-8) has been found to correlate with sperm DNA damage and sperm vitality in certain studies, but no other cytokine [ 16 ]. Alternatively, other studies found significantly increased levels of IL-2, IL-4, IL-6, IFN-γ, and, particularly, TNF-α in human semen in association with leukocytospermia [ 17 , 18 , 19 , 20 ].…”

Section: Sources Of Ros In the Human Ejaculatementioning

confidence: 99%

“…The one consistent factor linking leukocytospermia and defective sperm function is oxidative stress. The infiltration of leukocytes into the male tract has been associated with increases in biomarkers of peroxidative damage such as malondialdehyde [ 16 ] and the augmented generation of seminal ROS [ 27 ], as well as indicators of oxidative stress in spermatozoa including DNA fragmentation [ 16 ], 8OHdG formation in the sperm nucleus [ 28 ], and reduced sperm motility [ 29 ].…”

Section: Sources Of Ros In the Human Ejaculatementioning

confidence: 99%

“…The one element of this equation that does appear to be consistent is that the oxidative stress associated with leukocytospermia is involved in the suppression of sperm movement [ 39 , 40 , 41 ] as well as the induction of DNA damage [ 16 ]. Furthermore, the beneficial effects of antioxidants on sperm movement, including hyperactivation, suggests that such associations may be causative in nature, not merely correlative [ 42 ].…”

Section: Sources Of Ros In the Human Ejaculatementioning

confidence: 99%

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Male Infertility and Oxidative Stress: A Focus on the Underlying Mechanisms

et al. 2022

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Reactive oxygen species (ROS) play a critical role in defining the functional competence of human spermatozoa. When generated in moderate amounts, ROS promote sperm capacitation by facilitating cholesterol efflux from the plasma membrane, enhancing cAMP generation, inducing cytoplasmic alkalinization, increasing intracellular calcium levels, and stimulating the protein phosphorylation events that drive the attainment of a capacitated state. However, when ROS generation is excessive and/or the antioxidant defences of the reproductive system are compromised, a state of oxidative stress may be induced that disrupts the fertilizing capacity of the spermatozoa and the structural integrity of their DNA. This article focusses on the sources of ROS within this system and examines the circumstances under which the adequacy of antioxidant protection might become a limiting factor. Seminal leukocyte contamination can contribute to oxidative stress in the ejaculate while, in the germ line, the dysregulation of electron transport in the sperm mitochondria, elevated NADPH oxidase activity, or the excessive stimulation of amino acid oxidase action are all potential contributors to oxidative stress. A knowledge of the mechanisms responsible for creating such stress within the human ejaculate is essential in order to develop better antioxidant strategies that avoid the unintentional creation of its reductive counterpart.

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Section: Sources Of Ros In the Human Ejaculatementioning

confidence: 99%

Section: Sources Of Ros In the Human Ejaculatementioning

confidence: 99%

Section: Sources Of Ros In the Human Ejaculatementioning

confidence: 99%

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Male Infertility and Oxidative Stress: A Focus on the Underlying Mechanisms

et al. 2022

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“…Although still under debate, a strong association has been reported between infected semen and the presence of leukocytes in semen and male infertility [ 28 29 30 31 ]. Dysregulation of spermatogenesis due to the presence of microorganisms and activation of seminal leukocytes can impair semen quality [ 28 31 ].…”

Section: Male Genital Tract Infectionsmentioning

confidence: 99%

“…Leukocytospermia is found in 10% to 20% of infertile men [ 3 ]. There are numerous reports indicating that leukocytospermia has a negative impact on male fertility [ 7 12 13 29 ]. MGTI and leukocytospermia have been reported to be associated with high levels of ROS which in turn may cause a decline in sperm parameters including sperm concentration and motility [ 65 ].…”

Section: Leukocytospermia and Seminal Oxidative Stressmentioning

confidence: 99%

Relevance of Leukocytospermia and Semen Culture and Its True Place in Diagnosing and Treating Male Infertility

Sharma

Gupta

Agarwal

et al. 2022

World J Mens Health

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The current WHO 2010 manual for human semen analysis defines leukocytospermia as the presence of peroxidase-positive leukocytes at a concentration >1×10 6 /mL of semen. Granular leukocytes when activated are capable of generating high levels of reactive oxygen species in semen resulting in oxidative stress. Oxidative stress has been correlated with poor sperm quality, increased level of sperm DNA fragmentation and low fertility potential. The presence of leukocytes and pathogens in the semen may be a sign of infection and/or localized inflammatory response in the male genital tract and the accessory glands. Common uro-pathogens including Chlamydia trachomatis , Ureaplasma urealyticum , Neisseria gonorrhoeae , Mycoplasma hominis , and Escherichia coli can cause epididymitis, epididymo-orchitis, or prostatitis. The relationship between leukocytospermia and infection is unclear. Therefore, we describe the pathogens responsible for male genital tract infections and their association with leukocytospermia. The review also examines the diagnostic tests available to identify seminal leukocytes. The role of leukocytospermia in male infertility and its management is also discussed.

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Role of Infection and Leukocytes in Male Infertility

Das

Roychoudhury

et al. 2022

Advances in Experimental Medicine and Biology

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Relationship between nuclear DNA fragmentation, mitochondrial DNA damage and standard sperm parameters in spermatozoa of infertile patients with leukocytospermia

Cited by 16 publications

References 75 publications

Male Infertility and Oxidative Stress: A Focus on the Underlying Mechanisms

Male Infertility and Oxidative Stress: A Focus on the Underlying Mechanisms

Relevance of Leukocytospermia and Semen Culture and Its True Place in Diagnosing and Treating Male Infertility

Role of Infection and Leukocytes in Male Infertility

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