Relationship between mRNA biomarker candidates and location near a marine municipal wastewater outfall in the benthic indicator species Modiolus modiolus (L.)

Veldhoen, Nik; Kobylarz, Marek; Lowe, Christopher J.; Meloche, Lizanne M.; deBruyn, Adrian M.H.; Helbing, Caren C.

doi:10.1016/j.aquatox.2011.05.020

Cited by 9 publications

(9 citation statements)

References 34 publications

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“…All of these factors are related to cellular aging in bivalves (Ivanina et al 2008). Similar results were obtained for the expression of the HSP70 gene in the mussel Modiolus modiolus obtained from a bay with municipal marine outfalls, where an overexpression of mRNA was seen in the hepatopancreas (Veldhoen et al 2009) and adductor muscle (Veldhoen et al 2011). Experimental exposure to heavy metals such as Cu, Pb and Hg also increased the expression of HSP70 in the digestive gland and gills of the mussel Dreissena polymorpha (Navarro et al 2011).…”

Section: Relative Gene Expressionsupporting

confidence: 70%

Trace metal and biomarker levels in tissues ofArgopecten purpuratusin the north of Chile, and the potential use of this species as a bioindicator of metallic stress

Zapata

Lang

Riso

et al. 2012

Aquat. Living Resour.

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Trace metal and biomarker levels in tissues of Argopecten purpuratus in the north of Chile, and the potential use of this species as a bioindicator of metallic stress Abstract -The capacity to bioaccumulate trace metals present in San Jorge Bay, Antofagasta, Chile, was determined in northern scallop, Argopecten purpuratus, to examine the value of this important commercially species as a bioindicator of heavy metal pollution in areas where it is cultured. Scallops were sampled in summer 2009 in four sites: three natural populations (Coloso, Historic District and La Rinconada, marine reserve), and a commercial hatchery (Colorado). The concentrations of three heavy metals (copper, cadmium and lead) were then determined through stripping chronopotentiometric methods, and the levels of four biomarkers: three genes implicated in the stress and oxidative metabolism, i.e., glutathione peroxidase (GPx), glutathione s-transferase (GST) and heat shock protein 70 (HSP70), and a protein marker in the digestive gland and gill, a metallothionein (MT). The Historic District, located in the downtown area of the city, showed the highest metal concentration of all the sampled sites, as well as the highest levels of the four biomarkers. This can be largely attributed to the nearby international port area of the town and high sea traffic flow, exacerbated by the prevailing winds.

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Section: Relative Gene Expressionsupporting

confidence: 70%

Trace metal and biomarker levels in tissues ofArgopecten purpuratusin the north of Chile, and the potential use of this species as a bioindicator of metallic stress

Zapata

Lang

Riso

et al. 2012

Aquat. Living Resour.

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Section: Methodsmentioning

confidence: 99%

“…qPCR assays using SYBR Green I-based detection were conducted on a MX3005P Real-Time PCR System (Agilent Technologies Canada Inc., Mississauga, ON, Canada), as described previously. 30 For all expressed gene sequences investigated, quadruplicate amplification reactions (15 μL) were performed for each liver sample. The amplification thermocycle program for each transcript consisted of an initial enzyme activation step at 95 °C (9 min) followed by 40 cycles of 95 °C denaturation (15 s), 60 °C (or 57 °C for Rara, Tshb, Il1b) annealing (30 s), and 72 °C elongation (45 s).…”

Section: ■ Introductionmentioning

confidence: 99%

PCB Related Effects Thresholds As Derived through Gene Transcript Profiles in Locally Contaminated Ringed Seals (Pusa hispida)

Brown

Ross

Reimer

et al. 2014

Environ. Sci. Technol.

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Causal evidence linking toxic injury to polychlorinated biphenyl (PCB) exposure is typically confounded by the complexity of real-world contaminant mixtures to which aquatic wildlife are exposed. A local PCB "hotspot" on the Labrador coast provided a rare opportunity to evaluate the effects of PCBs on the health of a marine mammal as this chemical dominated their persistent organic pollutant (POP) burdens. The release of approximately 260 kg of PCBs by a military radar facility over a 30 year period (1970-2000) contaminated some local marine biota, including the ringed seal (Pusa hispida). The abundance profiles of eight health-related gene transcripts were evaluated in liver samples collected from 43 ringed seals in the affected area. The mRNA transcript levels of five gene targets, including aryl hydrocarbon receptor (Ahr), interleukin-1 β (Il1b), estrogen receptor α (Esr1), insulin like growth factor receptor 1 (Igf1), and glucocorticoid receptor α (Nr3c1) correlated with increasing levels of blubber PCBs. PCB threshold values calculated using best-fit hockey-stick regression models for these five genes averaged 1,680±206 ng/g lw, with the lowest, most conservative, being 1,370 ng/g lw for Il1b. Approximately 14% of the seals in the region exceeded this threshold. The dominance of PCBs in the seals studied enabled an assessment of the effects of this chemical on gene transcripts involved in regulating the health of a highly mobile predator, something that is rarely possible in the world of complex mixtures.

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“…The bivalve mollusks are widely used as indicator species in ecotoxicological biomonitoring programs [1][2][3]. Mollusk health status assessment through integrative biomarker indices is widely used in the biomonitoring programs as such a 'Mussel Watch' developed to detect nature consequences of the 'Prestige' oil spill [4].…”

Section: Introductionmentioning

confidence: 99%

“…Mollusk health status assessment through integrative biomarker indices is widely used in the biomonitoring programs as such a 'Mussel Watch' developed to detect nature consequences of the 'Prestige' oil spill [4]. Horse mussels Modiolus modiolus (L.) are a deep-sea benthic species readily accumulating oil and associated KarRC polycyclic aromatic hydrocarbons (PAHs) [2,5] from contaminated marine and coastal environments including due to sporadic spills [6]. Mussels respond to pollutant uptake through activating molecular mechanisms maintaining cell viability under pollutantinduced oxidative stress, toxicity, cellular stress threatening the structural and functional integrity of macromolecules, organelles, and tissues.…”

Section: Introductionmentioning

confidence: 99%

Integrative Biomarker Indices in a Benthic Indicator Species Modiolus Modiolus (L.) Under a Simulated Oil Spill

Лысенко

Sukhovskaya

Канцерова

et al. 2022

KSS

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The horse mussel Modiolus modiolus (L.) is a sentinel bivalve species used in monitoring programs to assess potential biological exposure to anthropogenic contaminants, including oil hydrocarbons, in benthic environments. In an aquarium based experiment, these mussels were exposed to a simulated oil spill where crude oil at realistic concentrations (from low to high) interacted with an inert environment (seawater, gravel surface, etc.) and the biota. Using a combination of endpoints that included tissue contaminant load, protease activity, antioxidant enzyme activity, and low-molecular antioxidant profiles, we characterized M. modiolus responses to this simulated crude oil spill. Significant differences were observed in tissue protein reserves, protease activity, and oxidative stress markers including glutathione content and glutathione-S-transferase activity in the hepatopancreas and gill tissues of the bivalves treated with oil. Total concentrations of oil-derived polycyclic aromatic hydrocarbons in M. modiolus tissues were generally highest under the highest ambient oil concentration with much lower levels at the lowest. This general pattern does match the activity of protein quality control proteases but not the antioxidant enzyme activity profiles. Glutathione-S-transferase activity in bivalves showed decreased activity under the high compared to the lower oil load and oil-free animals, while glutathione content and calpain activity were positively correlated with oil uptake. The data suggest that these benthic organisms were exhibiting biological responses to the oil polycyclic aromatic hydrocarbons and support the bioindicator value of the species. Keywords: M. modiolus, oil spill, PAHs, integrative biomarker approach, protein quality control, GSH, GST

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Relationship between mRNA biomarker candidates and location near a marine municipal wastewater outfall in the benthic indicator species Modiolus modiolus (L.)

Cited by 9 publications

References 34 publications

Trace metal and biomarker levels in tissues ofArgopecten purpuratusin the north of Chile, and the potential use of this species as a bioindicator of metallic stress

Trace metal and biomarker levels in tissues ofArgopecten purpuratusin the north of Chile, and the potential use of this species as a bioindicator of metallic stress

PCB Related Effects Thresholds As Derived through Gene Transcript Profiles in Locally Contaminated Ringed Seals (Pusa hispida)

Integrative Biomarker Indices in a Benthic Indicator Species Modiolus Modiolus (L.) Under a Simulated Oil Spill

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