Relationship between MRI <i>T</i><sub>1</sub>rho value and histological findings of intact and radially incised menisci in microminipigs

Nakagawa, Yusuke; Sekiya, Ichiro; Kondo, Shimpei; Tabuchi, Takashi; Ichinose, Shizuko; Koga, Hideyuki; Tsuji, Kunikazu; Muneta, Takeshi

doi:10.1002/jmri.24988

Cited by 15 publications

(10 citation statements)

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“…T1rho mapping provides objective information of biochemical components such as glycosaminoglycan, collagen, and water in cartilaginous tissues . We recently demonstrated a positive correlation between the T1rho value and histological scores in the torn meniscus of pigs . T1rho mapping might be one of the methods to quantify the quality of the regenerated meniscus and articular cartilage.…”

Section: Discussionmentioning

confidence: 99%

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Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates

et al. 2017

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Transplantation of aggregates of synovial mesenchymal stem cells (MSCs) enhanced meniscus regeneration in rats. Anatomy and biological properties of the meniscus depend on animal species. To apply this technique clinically, it is valuable to investigate the use of animals genetically close to humans. We investigated whether transplantation of aggregates of autologous synovial MSCs promoted meniscal regeneration in aged primates. Chynomolgus primates between 12 and 13 years old were used. After the anterior halves of the medial menisci in both knees were removed, an average of 14 aggregates consisting of 250,000 synovial MSCs were transplanted onto the meniscus defect. No aggregates were transplanted to the opposite knee for the control. Meniscus and articular cartilage were analyzed macroscopically, histologically, and by MRI T1rho mapping at 8 (n = 3) and 16 weeks (n = 4). The medial meniscus was larger and the modified Pauli's histological score for the regenerated meniscus was better in the MSC group than in the control group in each primate at 8 and 16 weeks. Mankin's score for the medial femoral condyle cartilage was better in the MSC group than in the control group in all primates at 16 weeks. T1rho value for both the regenerated meniscus and adjacent articular cartilage in the MSC group was closer to the normal meniscus than in the control group in all primates at 16 weeks. Transplantation of aggregates of autologous synovial MSCs promoted meniscus regeneration and delayed progression of degeneration of articular cartilage in aged primates. This is the first report dealing with meniscus regeneration in primates. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1274-1282, 2017.

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Section: Discussionmentioning

confidence: 99%

“…Quantification of MRI analysis was performed for the regenerated meniscus and the cartilage of the medial femoral condyle using the sagittal T1 rho mapping. The T1 rho values were calculated with Ziostation2 software (Ziosoft, Tokyo, Japan) …”

Section: Methodsmentioning

confidence: 99%

Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates

et al. 2017

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“…Harteveld et al (2016) and Van Der Kolk et al (2015) manually matched histological sections to their corresponding MR planes using the marked locations from fiducials in the MR images, ink markings in the histologic sections, and gross morphologic features. Nakagawa et al (2016) visually identified the histological sections that were morphologically close to the T 1ρ mapping image, and only those were stained. Koh et al (2016) compared the tissue sections of the largest cut surface of the tumour side-by-side with MR imaging.…”

Section: Slice-to-slice Approaches (2d-2d)mentioning

confidence: 99%

A Survey of Methods for 3D Histology Reconstruction

Pichat

Iglesias

Yousry³

et al. 2018

Medical Image Analysis

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Histology permits the observation of otherwise invisible structures of the internal topography of a specimen. Although it enables the investigation of tissues at a cellular level, it is invasive and breaks topology due to cutting. Three-dimensional (3D) reconstruction was thus introduced to overcome the limitations of single-section studies in a dimensional scope. 3D reconstruction finds its roots in embryology, where it enabled the visualisation of spatial relationships of developing systems and organs, and extended to biomedicine, where the observation of individual, stained sections provided only partial understanding of normal and abnormal tissues. However, despite bringing visual awareness, recovering realistic reconstructions is elusive without prior knowledge about the tissue shape. 3D medical imaging made such structural ground truths available. In addition, combining non-invasive imaging with histology unveiled invaluable opportunities to relate macroscopic information to the underlying microscopic properties of tissues through the establishment of spatial correspondences; image registration is one technique that permits the automation of such a process and we describe reconstruction methods that rely on it. It is thereby possible to recover the original topology of histology and lost relationships, gain insight into what affects the signals used to construct medical images (and characterise them), or build high resolution anatomical atlases. This paper reviews almost three decades of methods for 3D histology reconstruction from serial sections, used in the study of many different types of tissue. We first summarise the process that produces digitised sections from a tissue specimen in order to understand the peculiarity of the data, the associated artefacts and some possible ways to minimise them. We then describe methods for 3D histology reconstruction with and without the help of 3D medical imaging, along with methods of validation and some applications. We finally attempt to identify the trends and challenges that the field is facing, many of which are derived from the cross-disciplinary nature of the problem as it involves the collaboration between physicists, histolopathologists, computer scientists and physicians.

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“…Semi-thin sections at 1 μm were collected on glass slides and stained for 30 seconds with Toluidine Blue and then examined by a light microscope. Ultrathin sections at 90 nm were collected on copper grids, double-stained with uranyl acetate and lead citrate, and then examined by transmission electron microscopy (H-7100, Hitachi, Tokyo, Japan) [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Cartilage Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin In Vitro and In Vivo

et al. 2016

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ObjectiveLubricin expression in the superficial cartilage will be a crucial factor in the success of cartilage regeneration. Mesenchymal stem cells (MSCs) are an attractive cell source and the use of aggregates of MSCs has some advantages in terms of chondrogenic potential and efficiency of cell adhesion. Lubricin expression in transplanted MSCs has not been fully elucidated so far. Our goals were to determine (1) whether cartilage pellets of human MSCs expressed lubricin in vitro chondrogenesis, (2) whether aggregates of human MSCs promoted lubricin expression, and (3) whether aggregates of MSCs expressed lubricin in the superficial cartilage after transplantation into osteochondral defects in rats.MethodsFor in vitro analysis, human bone marrow (BM) MSCs were differentiated into cartilage by pellet culture, and also aggregated using the hanging drop technique. For an animal study, aggregates of BM MSCs derived from GFP transgenic rats were transplanted to the osteochondral defect in the trochlear groove of wild type rat knee joints. Lubricin expression was mainly evaluated in differentiated and regenerated cartilages.ResultsIn in vitro analysis, lubricin was detected in the superficial zone of the pellets and conditioned medium. mRNA expression of Proteoglycan4 (Prg4), which encodes lubricin, in pellets was significantly higher than that of undifferentiated MSCs. Aggregates showed different morphological features between the superficial and deep zone, and the Prg4 mRNA expression increased after aggregate formation. Lubricin was also found in the aggregate. In a rat study, articular cartilage regeneration was significantly better in the MSC group than in the control group as shown by macroscopical and histological analysis. The transmission electron microscope showed that morphology of the superficial cartilage in the MSC group was closer to that of the intact cartilage than in the control group. GFP positive cells remained in the repaired tissue and expressed lubricin in the superficial cartilage.ConclusionCartilage derived from MSCs expressed lubricin protein both in vitro and in vivo. Aggregation promoted lubricin expression of MSCs in vitro and transplantation of aggregates of MSCs regenerated cartilage including the superficial zone in a rat osteochondral defect model. Our results indicate that aggregated MSCs could be clinically relevant for therapeutic approaches to articular cartilage regeneration with an appropriate superficial zone in the future.

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Relationship between MRI T₁rho value and histological findings of intact and radially incised menisci in microminipigs

Cited by 15 publications

References 30 publications

Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates

Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates

A Survey of Methods for 3D Histology Reconstruction

Cartilage Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin In Vitro and In Vivo

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Relationship between MRI T1rho value and histological findings of intact and radially incised menisci in microminipigs

Cited by 15 publications

References 30 publications

Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates

Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates

A Survey of Methods for 3D Histology Reconstruction

Cartilage Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin In Vitro and In Vivo

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Relationship between MRI T₁rho value and histological findings of intact and radially incised menisci in microminipigs