RELATIONSHIP BETWEEN MORPHOLOGY, RHEOLOGY AND GLUCOAMYLASE PRODUCTION BY Aspergillus awamori IN SUBMERGED CULTURES

Pamboukian, Celso Ricardo Denser; Facciotti, Maria Cândida Reginato; Schmidell, Willibaldo

doi:10.1590/s0104-66321998000300004

Cited by 8 publications

(7 citation statements)

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“…As discussed above, another parameter that needs attention with respect to its effect on the fermentation run and downstream processing is the fungal morphology (pellet size). It is well known that a uniform small pellet size is preferred in many fungal fermentations due to its easier downstream processing and a better oxygen and nutrient mass transfer during the course of the fermentation [26]. Besides the PG enzyme production, the fungal biomass was also considered due to its significance in applications of waste treatment and feed stocks.…”

Section: Resultsmentioning

confidence: 99%

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Tarı

Gogus

Tokatlı

2007

Enzyme and Microbial Technology

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A two-step optimization procedure using central composite design with four factors (concentrations of maltrin and corn steep liquor (CSL), agitation speed and inoculation ratio) was used in order to investigate the effect of these parameters on the polygalacturonase (PG) enzyme activity, mycelia growth (biomass) and morphology (pellet size) of Aspergillus sojae ATCC 20235. According to the results of response surface methodology (RSM), initial concentrations of maltrin and CSL and agitation speed were significant (p < 0.05) on both PG enzyme production and biomass formation. As a result of this optimization, maximum PG activity (13.5 U/ml) was achievable at high maltrin (120 g/l), at low CSL (0 g/l), high agitation speed (350 rpm) and high inoculation ratio (2 × 10 7 total spore). Similarly, maximum biomass (26 g/l) could be obtained under the same conditions with only the difference for higher level of CSL requirement. The diameter of pellets in all optimization experiments ranged between 0.05 and 0.76 cm. The second optimization step improved the PG activity by 74% and the biomass by 40%.

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Section: Resultsmentioning

confidence: 99%

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Tarı

Gogus

Tokatlı

2007

Enzyme and Microbial Technology

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“…The inoculum was prepared according to a modification of the method of Pamboukian et al (1998) -1 ) were seeded with a metal strap, and then incubated at 28°C for 7 days, until the surface had been completely covered and sporulation had occurred. Afterwards, 10 mL of a solution of TWEEN 80 (0.1% v/v) were added to obtain the spore suspension.…”

Section: Substrate and Inoculum Preparationmentioning

confidence: 99%

LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Costa

Hermann

García-Román

et al. 2017

Braz. J. Chem. Eng.

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-Filamentous fungi can easily degrade agro-industrial wastes in solid-state fermentation processes, synthesizing many important commercial biocompounds, such as lipolytic enzymes. The aim of this study was to evaluate the effect of the composition of the solid culture medium on the production of lipolytic enzymes by the fungus Aspergillus niger. Rice, wheat and soybean bran were mixed to prepare the culture medium, which was supplemented with glucose, glycerol or soybean oil. Four mixture experimental designs were used to find the best medium for enzyme production. According to our results, the highest lipolytic activity values were achieved with a mixture of rice bran and glycerol (19.844 U·g -1 ) or with rice bran only (13.267 U·g -1 ). Thus, the lipolytic enzyme could be produced without any additional carbon source apart from rice bran, although glycerol addition induced a higher production.

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“…There are number of reports showing that various fungal fermentations require different growth morphologies for optimum product yield [21,19]. For example pelleted growth is preferred for itaconic acid production by Aspergillus terrreus, whereas pelleted and filamentous growth has been used for penicillin production industrially.…”

Section: Effect Of Type Of Strain and Production Media On Polygalactumentioning

confidence: 99%

Relationship between morphology, rheology and polygalacturonase production by Aspergillus sojae ATCC 20235 in submerged cultures

Gogus

Tarı

Oncu

et al. 2006

Biochemical Engineering Journal

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A full factorial statistical design, with the factors of, two taxonomically different strains, seven types of seed culture formulations (slants) and two types of fermentation media were used to investigate the effect of these parameters on the morphology and polygalacturonase production. The rheology of the final fermentation medium was analyzed and appropriate mathematical model was applied to calculate suspension viscosity. It was found that most fermentation broths showed non-Newtonian flow behavior. According to statistical analysis, factors of strain types and fermentation media and the interaction between them were found significant on the enzyme activity. The effect of seed culture formulations (slants) were found insignificant at the significance level of 1%. Interaction of slants with strain types and fermentation media were also found insignificant. Considering the morphology of the final culture, Aspergillus sojae with the desired pellet morphology in a complex media, inoculated with a seed culture prepared from molasses resulted in maximum polygalacturonase enzyme activity (0.2 U/ml) and lowest suspension viscosity with a broth rheology close to Newtonian flow behavior.

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RELATIONSHIP BETWEEN MORPHOLOGY, RHEOLOGY AND GLUCOAMYLASE PRODUCTION BY Aspergillus awamori IN SUBMERGED CULTURES

Cited by 8 publications

References 0 publications

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

Optimization of biomass, pellet size and polygalacturonase production by Aspergillus sojae ATCC 20235 using response surface methodology

LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Relationship between morphology, rheology and polygalacturonase production by Aspergillus sojae ATCC 20235 in submerged cultures

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