Relationship between male reproductive hormones, sperm DNA damage and markers of oxidative stress in infertility

Appasamy, Malathy; Muttukrishna, Shanthi; Pizzey, Arnold; Ozturk, Ozkan; Groome, NP; Serhal, Paul; Jauniaux, Eric

doi:10.1016/s1472-6483(10)60783-3

Cited by 101 publications

(81 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, we have found a highly significant negative correlation between FSH and viable sperm with normal MMP, which confirms the findings by Ruwanpura et al [36], obtained in experimental conditions. Similarly, the changes in MMP already observed in cases of inflammation [9,35,40,47] were correlated with FSH, the main hormonal regulator of spermatogenesis; however, this relation did not exist with DNA denaturation, which is in agreement with the findings of Appasamy et al [1]. We have observed a highly significant correlation between dead, apoptotic and viable sperm, viable sperm with normal MMP, sperm with DNA denaturation and conventional sperm parameters, which is in agreement with the findings in the literature [3,6,27,41].…”

Section: Discussionsupporting

confidence: 91%

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Zorn

Golob

Ihan

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To investigate the relationship between sperm apoptotic biomarkers and patient clinical characteristics, conventional sperm parameters and fertility potential. Material and methods Sperm analysis, phospholipid asymmetry, mitochondrial membrane potential (MMP) and DNA denaturation were assessed in 142 males of infertile couples. Seventy-three couples were allocated to the natural conception group, and 55 couples underwent IVF or ICSI. Results DNA denaturation correlated positively with age and negatively with testicular volume (TV). MMP correlated negatively with BMI and FSH and positively with TV. Normal viable sperm correlated positively with TV and negatively with age, BMI and FSH. DNA denaturation was associated with a significantly lower natural pregnancy rate (OR 5.4, p00.011). Conclusion Sperm apoptosis is related to male age, BMI, testicular volume and FSH. Among the apoptotic markers, only DNA denaturation has been found to predict natural pregnancy better than conventional sperm parameters.

show abstract

Section: Discussionsupporting

confidence: 91%

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Zorn

Golob

Ihan

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Koca et al suggested that antioxidant capacity is positively related to sperm motility and that decreased antioxidant capacity could impair sperm function due to increased ROS production or insufficient antioxidant capacity (27). Appasamy et al observed a negative correlation between antioxidant activity and sperm concentration, which suggests that oxidative stress could influence sperm concentration (28).…”

Section: Discussionmentioning

confidence: 99%

Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

Fazeli¹,

Salimi²

2016

Avicenna J Med Biochem

View full text Add to dashboard Cite

Background: Oxidative stress is the result of an imbalance between the production and scavenging of reactive oxygen species (ROS). Recently, oxidative stress has been introduced as a major cause of male infertility. Objectives: The aim of the present study was to determine the correlation between total antioxidant capacity (TAC) and malondialdehyde (MDA) as markers of oxidative stress in relation to idiopathic male infertility and sperm parameters. Patients and Methods: This case control study was conducted using 35 men with idiopathic infertility and 34 men with proven fertility. Seminal plasma TAC and MDA were measured by ferric reducing ability of plasma (FRAP) and thiobarbituric acid (TBA) reaction methods, respectively. Results: Seminal TAC levels were significantly lower and seminal MDA levels were significantly higher in men with idiopathic infertility than in fertile men (P < 0.0001 and P = 0.004, respectively). A positive correlation was shown between sperm motility, sperm morphology, and TAC levels in men with idiopathic infertility (P = 0.002 and P = 0.002, respectively). In addition, there was a correlation between sperm motility and TAC levels in fertile men (P = 0.005). There was no correlation between sperm count and TAC levels in either men with idiopathic infertility or in fertile men. Negative correlations were observed between MDA levels and sperm motility, morphology, and sperm count only in men with idiopathic infertility (P = 0.003, P = 0.001, and P = 0.006, respectively). Conclusions: Our results show that oxidative stress could play an important role in male infertility as well as in sperm motility and sperm morphology.

show abstract

“…20,27,28 However, it is unclear whether reduced semen antioxidant capacity necessarily causes sperm dysfunction (including sperm DNA damage). 1,3,29,30 Indeed, there is some controversy as to whether the high ROS levels detected in the semen of infertile men are due to increased ROS production, decreased ROS scavenging capacity or both. 21,31 If the high semen ROS levels are due (at least in part) to a decreased ROS scavenging capacity of semen, it would support the use of dietary antioxidant supplementation.…”

Section: Relationship Between Oxidative Stress and Sperm Dysfunctionmentioning

confidence: 99%

“…45,54,61 Several investigators have examined the effect of antioxidant therapy on sperm DNA integrity because sperm DNA damage may be caused, at least in part, by oxidative stress. 15,22,29,53,[62][63][64][65][66][67][68][69] In addition, sperm DNA damage is a more reliable outcome measure than sperm concentration or motility because measures of sperm DNA damage exhibit a lower degree of biological variability than conventional semen parameters. [70][71][72] Treatment with oral antioxidants has generally been associated with improvement in sperm DNA integrity and in some cases pregnancy rates after assisted reproduction, although most of these studies are small and few are randomized placebo-controlled trials ( Table 3).…”

Section: Relationship Between Oxidative Stress and Sperm Dysfunctionmentioning

confidence: 99%

Antioxidant therapy in male infertility: fact or fiction?

Zini¹,

Alhathal²

2011

Asian J Androl

110

View full text Add to dashboard Cite

Infertile men have higher levels of semen reactive oxygen species (ROS) than do fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This observation has led clinicians to treat infertile men with antioxidant supplements. The purpose of this article is to discuss the rationale for antioxidant therapy in infertile men and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm function and DNA damage. To date, most clinical studies suggest that dietary antioxidant supplements are beneficial in terms of improving sperm function and DNA integrity. However, the exact mechanism of action of dietary antioxidants and the optimal dietary supplement have not been established. Moreover, most of the clinical studies are small and few have evaluated pregnancy rates. A beneficial effect of in vitro antioxidant supplements in protecting spermatozoa from exogenous oxidants has been demonstrated in most studies; however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established conclusively. Keywords: antioxidant; male infertility; oxidative stress; sperm DNA fragmentation; vitamins RELATIONSHIP BETWEEN OXIDATIVE STRESS AND SPERM DYSFUNCTION Seminal oxidative stress (OS) results from an imbalance between reactive oxygen species (ROS) production and ROS scavenging by seminal antioxidants. Seminal OS is believed to be one of the main factors in the pathogenesis of sperm dysfunction and sperm DNA damage in male infertility.1-4 Indeed, it is estimated that 25% of infertile men possess high levels of semen ROS, whereas fertile men do not have high levels of semen ROS.1,4-6 Although a controlled production of these ROS is required for sperm physiology (sperm hyperactivation, capacitation and acrosome reaction) and for natural fertilization, [7][8][9] the excessive production of ROS by immature germ cells and leukocytes causes sperm dysfunction (lipid peroxidation, loss of motility and sperm DNA damage). 9,10 Spermatozoa are particularly susceptible to oxidative injury due to the abundance of plasma membrane polyunsaturated fatty acids. [10][11][12] These unsaturated fatty acids provide fluidity that is necessary for membrane fusion events (e.g., the acrosome reaction and sperm-egg interaction) and for sperm motility. However, the unsaturated nature of these molecules predisposes them to free radical attack and ongoing lipid peroxidation throughout the sperm plasma membrane. Once this process has been initiated, accumulation of lipid peroxides occurs on the sperm surface (this results in loss of sperm motility) and oxidative damage to DNA can ensue. 13,14SEMINAL ANTIOXIDANT CAPACITY AND SPERM DYSFUNCTION Seminal plasma and spermatozoa themselves are well endowed with an array of protective antioxidants to protect spermatozoa from OS, especially, at the post-testicular level. 6,15,16 Seminal plasma contains a number of high-molecular weigh...

show abstract

Relationship between male reproductive hormones, sperm DNA damage and markers of oxidative stress in infertility

Cited by 101 publications

References 42 publications

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

Antioxidant therapy in male infertility: fact or fiction?

Contact Info

Product

Resources

About