Relationship Between Main Channel Structure of Catalases and the Evolutionary Direction in Cold-Adapted Hydrogen Peroxide-Tolerant Exiguobacteium and Psychrobacter

Hanaoka, Yoshiko; Kimoto, Hideyuki; Yoshimume, Kazuaki; Hara, Isao; Matsuyama, Hidetoshi; Yumoto, Isao

doi:10.1007/s12088-020-00878-3

Cited by 1 publication

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References 27 publications

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“…Clade 3 catalases, i.e., BLC and MLC contain W (Phe) in the corresponding position of M 167 in EKTA. The volume of W (Phe) is 231.7 Å 3 , whereas that of M (Met) is 167.7 Å 3 [38,39]. Therefore, the size of the key residue M 167 in EKTA is the major reason for the high the compound I formation rate with peracetic acid.…”

Section: Relationship Between the Compound Formation Rate With Peracetic Acid And The Bottle Neck Amino Acid Residue In The Narrow Main Cmentioning

confidence: 99%

Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H₂O₂ Environments

Yumoto¹,

Hanaoka²,

Hara³

2021

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Enzymatic evolutionary strategies for adaptation to a high H2O2 environment have been evaluated using catalases with high catalytic efficiency isolated from two H2O2-tolerant bacteria, Exiguobacterium oxidotolerans and Psychrobacter piscatori. The entrance size of the narrow main channel in catalase has been estimated by determining the formation rate of the intermediate state of peracetic acid (b), which is a larger substrate than H2O2 versus that of catalase activity with H2O2 (a) (calculated as b/a). The ratio of b/a in E. oxidotolerans catalase (EKTA) is much higher than that of P. piscatori catalase (PKTA). To elucidate the structural differences between the catalases, the amino acids present in the main channel have been compared between the two catalases and other catalases in the database. The combination of amino acid residues, which contribute high catalytic efficiency in the narrow main channel of EKTA were different from those in PKTA. In this review, we discuss strategic differences in the elimination of high concentration of H2O2 owing to differences in the phylogenetic positions of catalases. In addition, we describe the relationships between the environmental distributions of genera involved in H2O2-resistant bacteria and their catalase functions based on the main channel structure of catalase.

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Section: Relationship Between the Compound Formation Rate With Peracetic Acid And The Bottle Neck Amino Acid Residue In The Narrow Main Cmentioning

confidence: 99%

Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H₂O₂ Environments

Yumoto¹,

Hanaoka²,

Hara³

2021

Antioxidants - Benefits, Sources, Mechanisms of Action

Self Cite

View full text Add to dashboard Cite

show abstract

Relationship Between Main Channel Structure of Catalases and the Evolutionary Direction in Cold-Adapted Hydrogen Peroxide-Tolerant Exiguobacteium and Psychrobacter

Cited by 1 publication

References 27 publications

Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H₂O₂ Environments

Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H₂O₂ Environments

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Relationship Between Main Channel Structure of Catalases and the Evolutionary Direction in Cold-Adapted Hydrogen Peroxide-Tolerant Exiguobacteium and Psychrobacter

Cited by 1 publication

References 27 publications

Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H2O2 Environments

Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H2O2 Environments

Contact Info

Product

Resources

About

Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H₂O₂ Environments

Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H₂O₂ Environments