Relationship between lysyl oxidase activity, pyridinoline content and muscle texture during ice storage of jumbo squid (Dosidicus gigas)

Abstract

Summary Collagen fibres, stabilised by lysyl oxidase (LOX), play an important role in jumbo squid because they are responsible for the union between various cells; therefore, a close interdependence between their functions and muscle firmness during ice storage has been suggested. In this study, the relationship between LOX activity, pyridinoline (Pyr) content and muscle texture (SF) during ice storage of jumbo squid mantle was evaluated. LOX activity was confirmed within the range of 4.1–7.1 × 10−3 U g−1 of protein, leading to an increase in Pyr content, detected in the range of 0.85–1.32 mmol mol−1 of collagen after 5–20 days. The SF of the muscle became harder during the ice storage time, increasing from 21.08 to 37.95 N. It was therefore possible to establish the relationship between LOX activity, collagen cross‐links (Pyr content) and texture patterns during ice storage of jumbo squid muscle, which increased after 20 days.

Relationship between muscle texture and the crosslinking degree of collagen fibers from octopus ( Octopus vulgaris ), guitarfish ( Rhinobatos productus ), and cazon ( Mustelus lunulatus )

“…Regarding Lox activity, the highest total activity was detected in the octopus crude extract ( p < .05), with no differences between guitarfish and cazon ( p ≥ .05) (Table ), while the specific activity presented the opposite behavior, with octopus having the lowest values ( p < .05). All the evaluated species showed lower specific activity than those described for jumbo squid ( D. gigas ), of 4.1 × 10 –3 nm H 2 O 2 /mg protein in the mantle (Ramírez‐Guerra, Fimbres‐Romero, et al, ) and 4.23 nM H 2 O 2 /mg protein in the arms (Torres‐Arreola et al, ). These differences in activity can be attributed to the differences that tend to exist between marine species, size, habitat, or catch season (Morales et al, ), which affects the arrangement of the crosslinking of collagen fibers (Torres‐Arreola et al, ) and the high concentration of Pyr (Ramírez‐Guerra, Fimbres‐Romero, et al, ).…”

“…These results suggest a great extent of crosslinking, especially in the octopus connective tissue matrix. As mentioned above, collagen fibers play an important role in maintaining the muscle firmness of marine species, which could be affected by the extent of their inter and intramolecular crosslinking (Ando et al, ; Ramírez‐Guerra, Fimbres‐Romero, et al, ; Torres‐Arreola et al, ).…”

“…Regarding Lox activity, the highest total activity was detected in the octopus crude extract (p < .05), with no differences between guitarfish and cazon (p ≥ .05) ( protein in the mantle (Ramírez-Guerra, Fimbres-Romero, et al, 2015) and 4.23 nM H 2 O 2 /mg protein in the arms (Torres-Arreola et al, 2012).…”

“…In 2017, were reported more than 55 million kg captured these three organisms which represent an important commercially value for the region (SAGARPA, 2017). The main reason to study this species is for their unique muscle texture that is harder compared with other marine organisms (Katsanidis & Agrafioti, 2009;Ocaño-Higuera et al, 2009;Ramírez-Guerra, Fimbres-Romero, et al, 2015). The muscle firmness (often expressed as shear force) and structural maintenance in cephalopods (Morales, Montero, & Moral, 2000;Osuna-Amarillas et al, 2017) are mainly due to the close union between cells as a result of the high crosslinked of the collagen fibers and the consequent accumulation of pyridinoline (Pyr) (Ramírez-Guerra, Fimbres-Romero, et al, 2015;Sarabia-Sainz et al, 2017).…”

“…Studies over the last decade have reported a close interdependence between muscle texture and the physicochemical properties of collagen in cephalopods during the postcapture storage of the species (Osuna-Amarillas et al, 2017;Ramírez-Guerra, Fimbres-Romero, et al, 2015). This relationship is influenced by the crosslinking degree (Pyr content) and Lox activity and suggests that collagen from octopus is more resistant to degradation than that from other marine species.…”