Relationship between <i>Bdellovibrio bacteriovorus</i> 6-5-S and autoclaved host bacteria

Crothers, S. F.; Fackrell, Hugh B.; Huang, Jiaoyan; Robinson, J.

doi:10.1139/m72-300

Cited by 15 publications

(8 citation statements)

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“…Several studies have indicated that bdellovibrios are capable of growth on heat-treated substrate cells (2,8,16,17,20), but, unlike our study, the bdellovibrio growth observed in these studies was extracellular or occurred by asynchronous multicycle intraperiplasmic development, or both. Multicycle intraperiplasmic growth can occur on Spirillum serpens (16) or E. coli cells (this study) that have been subjected to a pro-longed, low-temperature heat treatment (55 to 56°C for 60 to 90 min).…”

Section: Discussioncontrasting

confidence: 98%

“…Multicycle intraperiplasmic growth can occur on Spirillum serpens (16) or E. coli cells (this study) that have been subjected to a pro-longed, low-temperature heat treatment (55 to 56°C for 60 to 90 min). We observed little or no bdellovibrio attachment to E. coli cells that were heat-treated at 98°C (5 min or more), and, in agreement with other studies (2,16), only extracellular bdellovibrio growth occurred with these cells, provided the substrate cells were not washed after heat treatment. The substrate-cellderived bdellovibrio growth initiation factor (7,9) and other materials released into the suspending fluid during heat treatment provide the nutrients necessary for extracellular bdellovibrio growth, as has been observed for B. bacteriovorus 6-5-S (2).…”

Section: Discussionsupporting

confidence: 92%

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Intraperiplasmic Growth of Bdellovibrio bacteriovorus on Heat-Treated Escherichia coli

Hespell

1978

J Bacteriol

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Heat treatment (55°C for 40 min) of cell suspensions in buffer (ca. 3 x 109 cells per ml) of Escherichia coli ML35 caused a 4to 4.5-log loss of cell viability. Similar results were found for several other E. coli strains that were examined. As a result of this heat treatment, 260-nm-and 280-nm-absorbing materials were released into the suspending buffer, along with about 10% of the total cellular radioactivity, when cells uniformly labeled with 14C were used. In comparison with untreated cells, heat-treated E. coli ML35 cells showed (i) no significant changes in macromolecular composition other than ca. 22% less RNA content, (ii) an increased permeability to o-nitrophenyl-,8-D-galactopyranoside (a compound to which untreated cells are impermeable), (iii) almost complete loss of respiratory potential, and (iv) substantial losses of numerous glycolytic enzyme activities in cell extracts prepared from these cells. Intraperiplasmic development of Bdellovibrio bacteriovorus 109J with heat-treated E. coli ML35 as substrate cells appeared normal when observed microscopically, although bdellovibrio attachment and resultant bdelloplast formation were slightly retarded. No significant changes were observed in cell yields or in the ratios and contents of DNA, RNA, or protein between bdellovibrios harvested from untreated cells and those from heat-treated substrate cells after single-developmental-cycle growth on these cells. The average YATP values for intraperiplasmic growth on untreated and heattreated substrate cells were 16.0 and 17.9, respectively. It is concluded that intraperiplasmic bdellovibrio growth on gently heat-treated E. coli substrate cells is very similar to growth on untreated substrate cells, even though the former substrate cells are nonviable and substantially impaired in many metabolic activities.

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Section: Discussioncontrasting

confidence: 98%

Section: Discussionsupporting

confidence: 92%

Intraperiplasmic Growth of Bdellovibrio bacteriovorus on Heat-Treated Escherichia coli

Hespell

1978

J Bacteriol

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“…Numerous special terms have been introduced It has been demonstrated that the bdellovibrios into the literature to describe the behavior of the do not require intact prey cells to satisfy their Bdellovibrio under various growth conditions. nutritional requirements (3,8,11). Various au-Stolp and Starr (20) For personal use only.…”

Section: Discussionmentioning

confidence: 99%

Intracellular growth of Bdellovibrio bacteriovorus 6-5-S in heat-killed Spirillum serpens VHL

Ej¹,

Cf²,

Robinson³

1974

Can. J. Microbiol.

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1974. Intracellular growth of Bdellovibrio bacteriovorirs 6-5-S in heat-killed Spirillur?~ serpetis VHL. Can. J. Microbiol. 20: 847-851. If cells of Spirillum serpens (strain VHL) are heat-killed at 56C for 90 min the cells retain their original morphology remarkably well. When actively motile cells of Bdellovibrio bacteriovorus (strain 6-5-S) are inoculated into a suspension of S . serpens heat-killed at this temperature, B. bacteriovorus attacks and penetrares the dead cells, and undergoes a cycle of growth and replication similar to that which occurs in living cells. Intracellular growth of B. bacteriovorus in nonviable cells has not previously been reported. Ross, E. J., C. F. ROBINOW et J. ROBINSON. 1974. Intracellular growth of Bdellovibrio bacteriovorus 6-5-S in heat-killed Spirillum serpens VHL. Can. J. Microbiol. 20: 847-851. Si des cellules de Spirilllon serpens (souche VHL) sont dttruites par chauffage a 56C pendant 90 min, c: s dernieres conservent tres bien leur morphologie originelle. Lorsque des cellules trts mobiles, de Bdellouibrio bacteriovorus (souche 6-56) sont inoculees dans des suspensions de S . serpeus detruites ii cette temperature, B. bacteriovonls attaque et penetre les cellules mortes et debute un cycle de croissance et de replication semblable a celui que I'on retrouve dans les cellules vivantes. C'est la premiere fois que Yon rapporte la croissance intracellulaire de B. bacteriovorus dans des cellules nonviables.[Traduit par le journal]

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“…This conclusion has prompted several laboratories to attempt to induce the initiation of growth in attack-phase bdellovibrios by exposing them to various components of prey cell extracts (3,5,7,8,11).…”

mentioning

confidence: 99%

Prey-derived signals regulating duration of the developmental growth phase of Bdellovibrio bacteriovorus

Gray

Ruby

1990

J Bacteriol

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The filamentous elongation typical of growth-phase cells of the predatory bacterium Bdelovibrio bateriovorus is mediated by regulatory signals that are derived from the prey cell itself. These signals regulate the differentiation of growth-phase cells into the attack phase and appear to be required for continued filamentous growth by prey-dependent wild-type bdellovibrios and their prey-independent mutant derivatives alike. Using a prey-independent bdellovibrio strain, we have developed an assay for the detection and quantification of the growth-extending signal activity present in extracts of prey cells. This prey-derived regulatory activity was shown to be independent of its nutritional contribution to the bdellovibrios and was found to occur in heat-stable, proteinlike compounds of a variety of native molecular weights within the soluble fraction of extracts from both gram-negative and gram-positive bacteria.

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Relationship between Bdellovibrio bacteriovorus 6-5-S and autoclaved host bacteria

Cited by 15 publications

References 0 publications

Intraperiplasmic Growth of Bdellovibrio bacteriovorus on Heat-Treated Escherichia coli

Intraperiplasmic Growth of Bdellovibrio bacteriovorus on Heat-Treated Escherichia coli

Intracellular growth of Bdellovibrio bacteriovorus 6-5-S in heat-killed Spirillum serpens VHL

Prey-derived signals regulating duration of the developmental growth phase of Bdellovibrio bacteriovorus

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