Relationship between hemorheology and Glu(298)Asp polymorphism of endothelial nitric oxide synthase gene in patients with coronary artery disease

Abstract

This study aimed to investigate the relationship between endothelial nitric oxide synthase Glu(298)Asp gene polymorphism and hemorheological parameters. Red blood cell (RBC) deformability, aggregation were measured using an ectacytometry, whole blood, plasma viscosities were determined by a viscometer. Restriction fragment length polymorphism was used to detect polymorphism. Plasma nitrite, nitrate concentrations were determined by Griess method. The genotype distribution of the control group was as follows: 50 (67.5%) GG, 21 (28.4%) GT, 3 (4.1%) TT. A 48 (57.8%) of the patients with CAD had GG, 28 (33.7%) GT, 7 (8.5%) of them TT genotype. RBC aggregation index of CAD patients with G allele was higher and t(1/2) lower compared to controls carrying the same allele. The amplitude of RBC aggregation of healthy subjects with T allele, who are under increased cardiovascular risk was lower compared to control subjects with G allele. The results of this study indicate that, alterations in RBC aggregation seem to be a consequence of CAD, more than being a preexisting cause. Additionally, some compensatory mechanisms by causing decrements in RBC aggregation, may help regulation of circulation in healthy individuals with high cardiovascular risk.

Functional Effects of Endothelial Nitric Oxide Synthase Genetic Polymorphisms on Haemorheological Parameters in Healthy Human Individuals

“…Our findings, in opposite, suggest that 298Arg ⁄ Arg genotype might be favourable for maintaining blood flow by causing decreased and slower erythrocyte aggregation. In concurrence with our findings, Bor-Kucukatay et al (2009) recently reported diminished erythrocyte aggregation with TT genotype in patients with coronary artery disease [18].…”

“…The physiological consequences of eNOS genetic variability in erythrocyte rheology in human beings are largely unknown. Although few previous reports investigated the role of eNOS genetic variants on erythrocyte properties in microcirculatory disorders such as coronary artery disease, idiopathic sudden sensorineural hearing loss and systemic sclerosis, their results were inconclusive for physiological consequences of eNOS genetic polymorphisms in healthy individuals [16][17][18].Blood viscosity is determined by four parameters: haematocrit levels, erythrocyte aggregability, erythrocyte deformability and plasma viscosity [19]. Decrease in the deformability of erythrocytes or increase either in erythrocyte aggregation, plasma viscosity or haematocrit result in a higher blood viscosity.…”

Increased erythrocyte aggregation in patients with primary open angle glaucoma

“…It is worthy to note that increments in amplitude of the aggregation and aggregation index and decrement of t 1/2 are concordant with each other and indicate increment of RBC aggregation. The discussion whether alterations in haemorrheological parameters are the cause or the result of some systemic diseases, such as hypertension and coronary artery disease still goes on in the literature . Our experiments do not enlighten this issue directly for POAG because glaucoma is a local disease and far from affecting the determinants of RBC aggregation systemically.…”

Contribution ofeNOSVariants to the Genetic Susceptibility of Coronary Artery Disease in a Tunisian Population

“…Meta-analysis of clinical studies suggests that the eNOS gene polymorphisms could have a more predictive value in certain ethnic populations with specified cardiovascular risk factor profiles (Table 5) (Hingorani et al, 1999;Sigusch et al, 2000;Granath et al, 2001;Wang et al, 2001;Colombo et al, 2002;Afrasyap et al, 2004;Kerkeni et al, 2006;Yang and Ming, 2006;Angeline et al, 2010;Bor-Kucukatay et al, 2010;Isordia-Salas et al, 2010;Abdel Aziz et al, 2013).…”