Relationship between Glyoxylate Cycle and Nitrification Efficiency based on Heterotrophic Nitrification Bacterium Acinetobacter sp.Y1

Abstract

Abstract-The growth and ammonium removal ability of heterotrophic nitrification bacterium are strongly influenced by carbon sources, as well as the activities of key enzymes of glyoxylate cycle. To obtain a better insight into the relationship between glyoxylate cycle and nitrification, the two key enzymes of glyoxylate cycle and the ammonium removal ability of Acinetobacter sp.Y1 grew with different carbon sources were examined. Strain Y1 showed a wide organic carbon substrates, and grew but no nitrify in the medium with sucrose and glycerol as sole carbon source. The strain Y1 showed the most activities of isocitrate lyase and malate synthase when the ammonium removal rate reached highest, and no activities of the two enzymes were detected when the strain grew without nitrification phase. The positive correlation relationship was found between the glyoxylate cycle and nitrification.