Relationship between glutathione S-transferase, catalase, oxygen consumption, lipid peroxidation and oxidative stress in eggs and larvae of Boophilus microplus (Acarina: Ixodidae)

Freitas, Daniela Reis Joaquim de; Rosa, Renato Moreira; Moraes, João Antônio de; Campos, Eldo; Logullo, Carlos; Vaz, Itabajara da Silva; Masuda, Aoi

doi:10.1016/j.cbpa.2006.04.032

Cited by 57 publications

(47 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, group A embryos (embryos were exposed to ethanol for 48 hpf and then maintained in hatching solution) were analyzed at 96 and 144 hpf also showed no change after ethanol treatment. Enhancement of LPO during development was also reported in other species (Freitas et al, 2007). However, ethanol has been shown to induce LPO in fetal rat hepatocytes (Henderson et al, 1999) which are not in agreement with the results obtained here.…”

Section: Discussioncontrasting

confidence: 86%

Disruption of circulation by ethanol promotes fetal alcohol spectrum disorder (FASD) in medaka (Oryzias latipes) embryogenesis

Khan

Dasmahapatra

2008

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

Japanese medaka (Oryzias latipes) embryos exposed to ethanol have developed craniofacial, cardiovascular and skeletal defects which can be compared with the phenotypic features of fetal alcohol spectrum disorder (FASD) observed in human. The present experiment was designed to show that the disruption in circulation by ethanol during embryogenesis is a potential cause of FASD. Fertilized eggs were exposed to ethanol (0, 100 and/or 400 mM) for 24 or 48 h at various developmental stages (Iwamatsu stages 4-30) and were analyzed at 6 day post fertilization (dpf). It was observed that controls and the embryos exposed to 100 mM ethanol were in circulating state; however, a significant number of embryos of stages 4-24 exposed to 400 mM ethanol had disrupted circulation. Compared to controls, protein and RNA contents were significantly reduced in noncirculating embryos. Lipid peroxidation (LPO) analysis was made at 3, 6, 24, 48, 96 and 144 hour post fertilization (hpf). LPO was increased with the advancement of morphogenesis; however, ethanol or the circulation status had no effect. We further analyzed alcohol dehydrogenase (Adh 5 and adh8) and aldehyde dehydrogenase (Aldh9A and Aldh1A2) enzyme mRNAs in the embryos exposed to 400 mM ethanol for 24h. A developmental stage specific reduction in these enzyme mRNAs by ethanol was observed. We conclude that ethanol-induced disruption in circulation during embryogenesis is a potential cause of the development of FASD features in medaka.

show abstract

Section: Discussioncontrasting

confidence: 86%

Disruption of circulation by ethanol promotes fetal alcohol spectrum disorder (FASD) in medaka (Oryzias latipes) embryogenesis

Khan

Dasmahapatra

2008

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

show abstract

“…GSTs have been previously analyzed in ticks and shown to play a role in oxidant defenses in eggs (Freitas et al, 2007). GBSR01000133 appears to have similar identity in several tick species, implying that GSTs serve a crucial function for tick survival.…”

Section: Resultsmentioning

confidence: 99%

Identification of 24h Ixodes scapularis immunogenic tick saliva proteins

Lewis

Radulović

Kim

et al. 2015

Ticks and Tick-borne Diseases

View full text Add to dashboard Cite

Ixodes scapularis is arguably the most medically important tick species in the United States. This tick transmits 5 of the 14 human tick-borne disease (TBD) agents in the USA: Borrelia burgdorferi, Anaplasma phagocytophilum, B. miyamotoi, Babesia microti, and Powassan virus disease. Except for the Powassan virus disease, I. scapularis-vectored TBD agents require more than 24 h post attachment to be transmitted. This study describes identification of 24 h immunogenic I. scapularis tick saliva proteins, which could provide opportunities to develop strategies to stop tick feeding before transmission of the majority of pathogens. A 24 h fed female I. scapularis phage display cDNA expression library was biopanned using rabbit antibodies to 24 h fed I. scapularis female tick saliva proteins, subjected to next generation sequencing, de novo assembly, and bioinformatic analyses. A total of 182 contigs were assembled, of which ~19% (35/182) are novel and did not show identity to any known proteins in GenBank. The remaining ~81% (147/182) of contigs were provisionally identified based on matches in GenBank including ~18% (27/147) that matched protein sequences previously annotated as hypothetical and putative tick saliva proteins. Others include proteases and protease inhibitors (~3%, 5/147), transporters and/or ligand binding proteins (~6%, 9/147), immunogenic tick saliva housekeeping enzyme-like (17%, 25/147), ribosomal protein-like (~31%, 46/147), and those classified as miscellaneous (~24%, 35/147). Notable among the miscellaneous class include antimicrobial peptides (microplusin and ricinusin), myosin-like proteins that have been previously found in tick saliva, and heat shock tick saliva protein. Data in this study provides the foundation for in-depth analysis of I. scapularis feeding during the first 24 h, before the majority of TBD agents can be transmitted.

show abstract

“…GSTs are present in almost all eukaryotes, where they occur in multiple isoenzymic forms, constituting a significant intracellular mechanism of detoxification (Freitas et al, 2007). GSTs are phase-II detoxification mechanisms that often facilitate the excretion of primary oxidative and hydrolytic insecticide metabolites resulting from phase-I detoxification.…”

Section: Discussionmentioning

confidence: 99%

“…In Australia, detection of high-level resistance to phosphine in psocids infesting stored commodities has elevated their pest status enormously and put them alongside the major beetle pests (Nayak et al, 2003). Moreover, the importance of psocids in Australia has been enhanced in recent years, mostly due to the failure of almost all currently registered grain protectants against these pests (Nayak et al, 2005; Daglish, 2006, 2007).Glutathione S-transferases (GSTs, EC 2.5.1.18) belong to a supergene family of enzymes that are involved in detoxification of xenobiotics, protection from oxidative damage, and intracellular transport of hormones, endogenous metabolites, and exogenous chemicals (Hemingway et al, 1991;Huang et al, 1998;Freitas et al, 2007). In insect, GSTs are of primary interest because of their role in phase II detoxification of several chemical insecticide classes and their potential contributions to insecticide resistance in economically important pest species in diverse agronomic cropping systems, as well as insecticide resistance in disease vectors (Fournier et al, 1992;Yu, 2002).…”

mentioning

confidence: 97%

Purification and partial characterization of glutathione S‐transferase from insecticide‐resistant field populations of Liposcelis paeta Pearman (Psocoptera: Liposcelididae)

Dou

et al. 2009

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Enzymes that possess glutathione S-transferase (GST) activity were purified to homogeneity by glutathione-agarose affinity chromatography from three field populations of Liposcelis paeta (Pearman). These populations were collected from Nanyang city of Henan Province (NY), Wuzhou (WZ) and Hezhou (HZ) cities of Guangxi Province, China, and had different susceptibilities to dichlorvos [LC(50)s of the NY (281.48 mg/m(2)), the WZ (285.07 mg/m(2)), and the HZ (243.52 mg/m(2)), respectively]. The specific activities of purified enzymes from these three populations increased 32.24-, 99.81-, and 42.52-fold, respectively. Kinetic analyses showed that the catalytic activity of purified GST from NY population towards GSH was much higher than the others, while WZ population reached the highest in V(max) (CDNB). SDS-polyacrylamide electrophoresis revealed that the purified GST had two subunits with a molecular mass of 23.31 and 20.43 kDa for NY, 53.14 and 20.13 kDa for WZ, and 50.79 and 19.42 kDa for HZ, respectively. The in vitro inhibition studies of GSTs indicated that three kinds of insecticides (chlorpyrifos, carbosulfan, and cypermethrin) and five metallic ions (Zn(2+), Ba(2+), Ca(2+), Hg(2+), Mn(2+), and Mg(2+)) all possessed inhibitory effects on purified GST, and ethacrynic acid (EA, a specific inhibitor of GST) expressed inhibitory effects. In the bioassay, three populations of L. paeta had different susceptibilities to different insecticides, even after they were reared on diets consisting of 25% EA. The GST activities of L. paeta from different areas also showed different temperature and pH stabilities. The differences in GST among the three populations may be attributed partially to the differences in control practices for psocids between Henan and Guangxi Provinces. (c) 2009 Wiley Periodicals, Inc.

show abstract

Relationship between glutathione S-transferase, catalase, oxygen consumption, lipid peroxidation and oxidative stress in eggs and larvae of Boophilus microplus (Acarina: Ixodidae)

Cited by 57 publications

References 43 publications

Disruption of circulation by ethanol promotes fetal alcohol spectrum disorder (FASD) in medaka (Oryzias latipes) embryogenesis

Disruption of circulation by ethanol promotes fetal alcohol spectrum disorder (FASD) in medaka (Oryzias latipes) embryogenesis

Identification of 24h Ixodes scapularis immunogenic tick saliva proteins

Purification and partial characterization of glutathione S‐transferase from insecticide‐resistant field populations of Liposcelis paeta Pearman (Psocoptera: Liposcelididae)

Contact Info

Product

Resources

About