Relationship between FGF21 and UCP1 levels under time-restricted feeding and high-fat diet

Chapnik, Nava; Genzer, Yoni; Froy, Oren

doi:10.1016/j.jnutbio.2016.10.017

Cited by 25 publications

(25 citation statements)

References 35 publications

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“…Given that the liver FGF21, but not FGF21 in iWAT, was induced by E2 treatment, and the evidence that FGF21 stimulates iWAT browning through PGC-1a and mitochondrial oxidation through AMPK and sirtuin 1 (22), we hypothesized that E2-inducing iWAT browning could be mediated by liver FGF21. Based on the FGF21LKO OVX mouse model, our data found that browning and reduction effects on iWAT by E2 treatment was absent in FGF21LKO mice, which agrees with previous reports that demonstrated FGF21 has a critical, physiologic role in the thermogenic recruitment of white adipose tissues via PGC-1a (22,39).…”

Section: Discussionsupporting

confidence: 92%

Identification of hepatic fibroblast growth factor 21 as a mediator in 17β‐estradiol‐induced white adipose tissue browning

et al. 2018

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Both ovarian E2 and hepatic fibroblast growth factor 21 (FGF21) are critical for energy homeostasis and white adipose tissue browning. Estrogen receptor α (ERα) is abundantly expressed in liver. However, whether FGF21 has a role in E2-induced white adipose tissue browning remains uncertain. In this study, we showed that hepatic Fgf21 expression and secretion during estrus cycle changed with the tetradian oscillatory secretion of circulation E2 in adult, female mice, with their peak expressions and secretions at the proestrus. In addition, exogenous E2 robustly stimulated liver Fgf21 expression and elevated serum FGF21 concentrations, which induced browning gene expression and reduced the tissue weight in subcutaneous white adipose in mice with ovariectomies. The inhibitor of mammalian target of rapamycin (mTOR) and of ERα blocked the induction effect of E2 on the expression of Fgf21 in primary hepatocytes, which revealed that E2 might stimulate FGF21 expression via the ERα-mTOR pathway. Furthermore, FGF21 liver-specific deficiency abolished E2-induced white adipose browning in mice with ovariectomies. This study indicates that ovarian E2 increased liver FGF21 expression directly, which in turn, functioned as an endocrine signal to influence inguinal white adipose tissue browning.-Hua, L., Zhuo, Y., Jiang, D., Li, J., Huang, X., Zhu, Y., Li, Z., Yan, L., Jin, C., Jiang, X., Che, L., Fang, Z., Lin, Y., Xu, S., Li, J., Feng, B., Wu, D. Identification of hepatic fibroblast growth factor 21 as a mediator in 17β-estradiol-induced white adipose tissue browning.

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Section: Discussionsupporting

confidence: 92%

Identification of hepatic fibroblast growth factor 21 as a mediator in 17β‐estradiol‐induced white adipose tissue browning

et al. 2018

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“…In our study, plasma FGF21 concentrations were consistently lower at the three sampling times in rats fed hypocaloric diets than in rats fed normal and high calories. Several factors may explain the discrepancy with studies that have found an increase in FGF21 after calorie restriction: (a) Previous studies have been conducted in mice which may behave differently to rats, (b) time of sampling may be important because FGF21 has been shown to be affected by circadian rhythm [ 35 ] and in some studies, e.g., Thompson et al [ 34 ], mice were sampled at night time, while in our study the rats were sampled in the morning, between 9.00 and 11.00 h. In our opinion, the most important factor to explain the FGF21 concentrations detected in rats fed LC is the fact that these rats were subjected to calorie restriction but not to protein restriction, which seems the main trigger of FGF21 elevation [ 32 ]. In fact, the LC diet used in the present study has a high protein (52%) content.…”

Section: Discussionmentioning

confidence: 99%

Hypocaloric Diet Prevents the Decrease in FGF21 Elicited by High Phosphorus Intake

et al. 2018

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The effect of dietary phosphorus (P) on fibroblast growth factor 21 (FGF21)/β-klotho axis was investigated in rats that were fed diets with: Normal (NP) or high P (HP) and either normal (NC), high (HC) or low calories (LC). Sampling was performed at 1, 4 and 7 months. Plasma FGF21 concentrations were higher (p < 0.05) in NC and HC than in LC groups. Increasing P intake had differing effects on plasma FGF21 in rats fed NC and HC vs. rats fed LC at the three sampling times. When compared with the NP groups, FGF21 concentrations decreased at the three sampling points in rats fed NC-HP (80 vs. 194, 185 vs. 382, 145 vs. 403 pg/mL) and HC-HP (90 vs. 190, 173 vs. 353, 94 vs. 434 pg/mL). However, FGF21 did not decrease in rats fed LC-HP (34 vs. 20, 332 vs. 164 and 155 vs. 81 pg/mL). In addition, LC groups had a much lower liver FGF21 messenger ribonucleic acid/glyceraldehyde 3-phosphate dehydrogenase (mRNA/GAPDH) ratio (0.51 ± 0.08 and 0.56 ± 0.07) than the NC-NP (0.97 ± 0.14) and HC-NP (0.97 ± 0.22) groups. Increasing P intake reduced liver FGF21 mRNA/GAPDH in rats fed NC and HC to 0.42 ± 0.05 and 0.37 ± 0.04. Liver β-klotho mRNA/GAPDH ratio was lower (p < 0.05) in LC groups (0.66 ± 0.06 and 0.59 ± 0.10) than in NC (1.09 ± 0.17 and 1.03 ± 0.14) and HC (1.19 ± 0.12 and 1.34 ± 0.19) groups. A reduction (p < 0.05) in β-klotho protein/α-tubulin ratio was also observed in LC groups (0.65 ± 0.05 and 0.49 ± 0.08) when compared with NC (1.12 ± 0.11 and 0.91 ± 0.11) and HC (0.93 ± 0.17 and 0.87 ± 0.09) groups. In conclusion β-klotho is potently regulated by caloric restriction but not by increasing P intake while FGF21 is regulated by both caloric restriction and increased P intake. Moreover, increased P intake has a differential effect on FGF21 in calorie repleted and calorie depleted rats.

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“…Previous TRF studies have involved access to food for 3-8 h daily [17][18][19][20][21], and we previously provided the purified diets, AIN-76 and AIN-93, to mice and rats daily for 4 h (2 h at both the beginning and end of the dark period). The body masses of these animals were lower than of those that had consumed the same diet ad libitum.…”

Section: Discussionmentioning

confidence: 99%

“…Results: Total energy consumptions by the TRF-4% and TRF-10% fat groups were 26.5% and 18.6%, respectively, less than that of the ad libitum-fed groups. The body mass gains by the 4% and 10% fat diet groups fed ad libitum were similar, but the gain by the TRF-4% fat group was markedly lower than that of the TRF-10% fat group.…”

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confidence: 94%

Suitability of a 10% fat diet for use in time-restricted feeding experiments with C57BL/6 mice

Matsuyama

Tanaka

Yokoyama

et al. 2020

BCHD

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Background: There is growing interest in the possible role of circadian rhythms in feeding behavior and their effect on diet-induced obesity. However, it is unclear whether widely used purified diets are suitable for use as normal control diets for rodents undergoing time-restricted feeding studies. In the present study, we compared the effects of 4% and 10% fat diets on body mass gain and food consumption during time-restricted feeding (TRF).Methods: Ad libitum-fed male C57BL/6J mice had free access to AIN-93M (4% fat) or modified 10% fat diets, whereas TRF groups were only able to consume one of these diets twice daily, at ZT23:00–01:00 and ZT11:00–13:00, for 5 weeks.Results: Total energy consumptions by the TRF-4% and TRF-10% fat groups were 26.5% and 18.6%, respectively, less than that of the ad libitum-fed groups. The body mass gains by the 4% and 10% fat diet groups fed ad libitum were similar, but the gain by the TRF-4% fat group was markedly lower than that of the TRF-10% fat group. In addition, whereas the body mass gain by the TRF-10% fat group was similar to that of its ad libitum equivalent, the gain by the TRF-4% fat group was much lower than that of the equivalent ad libitum-fed group.Conclusion: We showed for the first time that a 10% fat diet (21.9% kcal from fat) is suitable for the maintenance of body mass gain during TRF of C57BL/6 mice.Keywords: ad libitum feeding, fat content, mouse, time-restricted feeding

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Relationship between FGF21 and UCP1 levels under time-restricted feeding and high-fat diet

Cited by 25 publications

References 35 publications

Identification of hepatic fibroblast growth factor 21 as a mediator in 17β‐estradiol‐induced white adipose tissue browning

Identification of hepatic fibroblast growth factor 21 as a mediator in 17β‐estradiol‐induced white adipose tissue browning

Hypocaloric Diet Prevents the Decrease in FGF21 Elicited by High Phosphorus Intake

Suitability of a 10% fat diet for use in time-restricted feeding experiments with C57BL/6 mice

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