Relationship between feeding strategies and nitrogen sources in platform chemical bio-based 2,3-butanediol production in fed-batch fermentation

Tinôco, Daniel; de Castro, Rui de Paula Vieira; Teixeira, Douglas; de Assis Beltrão Junior, Francisco; de Oliveira Júnior, Eduardo; Coutinho, Paulo Luiz de Andrade; Freire, Denise Maria Guimarães

doi:10.1039/d3re00165b

React. Chem. Eng.

2023

DOI: 10.1039/d3re00165b

|View full text |Cite

Relationship between feeding strategies and nitrogen sources in platform chemical bio-based 2,3-butanediol production in fed-batch fermentation

Daniel Tinôco,

Rui de Paula Vieira de Castro,

Douglas Teixeira

et al.

Abstract: Bio-based 2,3-BDO production can be improved through fed-batch fermentation and nutritional control.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 50 publications

(89 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Sustainable production of optically pure platform chemical bio-based (R,R)−2,3-butanediol from sugarcane molasses in a low-cost salt medium

Tinôco,

Seldin,

Coutinho

et al. 2024

Industrial Crops and Products

View full text Add to dashboard Cite

Sustainable production of optically pure platform chemical bio-based (R,R)−2,3-butanediol from sugarcane molasses in a low-cost salt medium

Tinôco,

Seldin,

Coutinho

et al. 2024

Industrial Crops and Products

View full text Add to dashboard Cite

Strain and model development for auto- and heterotrophic 2,3-butanediol production using Cupriavidus necator H16

Weiler,

Jürgensen,

Cornejo Infante

et al. 2024

Biotechnol Biofuels

View full text Add to dashboard Cite

The production of platform chemicals from renewable energy sources is a crucial step towards a post-fossil economy. This study reports on the production of acetoin and 2,3-butanediol heterotrophically with fructose as substrate and autotrophically from CO2 as carbon source, H2 as electron donor and O2 as electron acceptor with Cupriavidus necator. In a previous study, the strain was developed for the production of acetoin with high carbon efficiency. Acetoin can serve as a precursor for the synthesis of 2,3-butanediol by the integration of a butanediol dehydrogenase. In this study, different plasmid backbones and butanediol dehydrogenases were evaluated regarding efficiency for CO2-based 2,3-butanediol production. The developed strain utilizes the pBBR1 plasmid bearing a 2,3-butanediol dehydrogenase from Enterobacter cloacae and is characterized by 2,3-butanediol as the main product and a heterotrophic total product yield of 88.11%, an autotrophic volumetric productivity of 39.45 mg L−1 h−1, a total product carbon yield of 81.6%, an H2 efficiency of 33.46%, and a specific productivity of 0.016 g product per gram of biomass per hour. In addition, a mathematical model was developed to simulate the processes under these conditions. With this model, it was possible to calculate productivities and substrate usage at distinct time points of the production processes and calculate productivities and substrate usage with high resolution which will be useful in future applications.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Relationship between feeding strategies and nitrogen sources in platform chemical bio-based 2,3-butanediol production in fed-batch fermentation

Abstract: Bio-based 2,3-BDO production can be improved through fed-batch fermentation and nutritional control.

Cited by 2 publications

References 50 publications

Sustainable production of optically pure platform chemical bio-based (R,R)−2,3-butanediol from sugarcane molasses in a low-cost salt medium

Sustainable production of optically pure platform chemical bio-based (R,R)−2,3-butanediol from sugarcane molasses in a low-cost salt medium

Strain and model development for auto- and heterotrophic 2,3-butanediol production using Cupriavidus necator H16

Contact Info

Product

Resources

About