Relationship between Ethylene Evolution and Sucrose Content in Excised Leaf Blades of Rice

Kobayashi, Hidekazu; Saka, Hitoshi

doi:10.1626/pps.3.398

Cited by 20 publications

(16 citation statements)

References 26 publications

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“…The results of the present study showed that mT-induced shoot formation in P. × hortorum 'Bergpalais' was inhibited by the sucrose supply. In contrast to other plant species, including tobacco (Philosoph-Hadas et al, 1985;Meir et al, 1989) and rice (Kobayashi and Saka, 2003), increased exogenous sucrose levels resulted in a decrease in ethylene production by Pelargonium shoots. In contrast to the study on Arabidopsis (Zhou et al, 1998;Gazzarini and McCourt, 2001), the sucrose-inhibition of Pelargonium shoot formation was not overcome by the application of ACC.…”

Section: Discussionmentioning

confidence: 70%

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Wojtania¹,

Wegrzynowicz-Lesiak²,

Dziurka³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium × hortorum 'Bergpalais' in vitro. Increasing the concentration of sucrose from 15 to 40 g L -1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to mT-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The mT-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO 3 ), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15-40 g L -1) in the presence of mT and AgNO 3 resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L -1 ) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of Pelargonium in vitro is mediated by ABA.K Ke ey y w wo or rd ds s: : Ethylene inhibitors, endogenous ABA, geranium, meta-topolin, sucrose concentration, shoot multiplication.

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Section: Discussionmentioning

confidence: 70%

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Wojtania¹,

Wegrzynowicz-Lesiak²,

Dziurka³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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“…9) in a dose-and signal-dependent manner, respectively, similar to leaves in other plants, such as tobacco (Philosoph-Hadas et al, 1985) and rice (Kobayashi and Saka, 2000). Suc appears to regulate ethylene production as well as anthocyanin biosynthesis (Fig.…”

Section: Suc-induced Production Of Ethylene Represses Anthocyanin Ovementioning

confidence: 62%

“…Exogenously supplied Suc enhances ethylene production in a dose-dependent manner in tobacco (Philosoph-Hadas et al, 1985) and rice (Oryza sativa; Kobayashi and Saka, 2000) leaves. Therefore, a timecourse analysis was used to investigate the induction kinetics of sugar, anthocyanin, and ethylene production in response to exogenous Suc treatment.…”

Section: Suc-induced Production Of Ethylenementioning

confidence: 99%

Ethylene Suppression of Sugar-Induced Anthocyanin Pigmentation in Arabidopsis

et al. 2010

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Anthocyanin accumulation is regulated negatively by ethylene signaling and positively by sugar and light signaling. However, the antagonistic interactions underlying these signalings remain to be elucidated fully. We show that ethylene inhibits anthocyanin accumulation induced by sucrose (Suc) and light by suppressing the expression of transcription factors that positively regulate anthocyanin biosynthesis, including GLABRA3, TRANSPARENT TESTA8, and PRODUCTION OF AN-THOCYANIN PIGMENT1, while stimulating the concomitant expression of the negative R3-MYB regulator MYBL2. Genetic analyses show that the ethylene-mediated suppression of anthocyanin accumulation is dependent upon ethylene signaling components responsible for the triple response. Furthermore, these positive and negative signaling pathways appear to be under photosynthetic control. Suc and light induction of anthocyanin accumulation was almost fully inhibited in wild-type Arabidopsis (Arabidopsis thaliana) ecotype Columbia and ethylene (ethylene response1 [etr1-1]) and light (long hypocotyl1 [hy1], cryptochrome1/2, and hy5) signaling mutants treated with the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. The transcript level of the sugar transporter gene SUC1 was enhanced in ecotype Columbia treated with the ethylene-binding inhibitor silver and in etr1-1, ethylene insensitive2 (ein2-1), and ein3 ein3-like1 mutants. In contrast, 3-(3,4-dichlorophenyl)-1,1-dimethylurea treatment reduced SUC1 expression, which indicates strongly that SUC1 represents an integrator for signals provided by sugar, light, and ethylene. SUC1 mutations lowered accumulations of anthocyanin pigment, soluble sugar content, and ethylene production in response to Suc and light signals. These data demonstrate that the suppression of SUC1 expression by ethylene inhibits Suc-induced anthocyanin accumulation in the presence of light and, hence, fine-tunes anthocyanin homeostasis.Anthocyanins play key roles in many plant physiological processes; for instance, they form photoprotective screens in vegetative tissue, act as visual attractors to aid pollination and seed dispersal, and function as antimicrobial agents and feeding deterrents in the defense response (Winkel-Shirley, 2001;Steyn et al., 2002). The anthocyanin biosynthetic pathway is well described in plants. In Arabidopsis (Arabidopsis thaliana) and other plants, including Antirrhinum majus (snapdragon) and Petunia hybrida (petunia), early biosynthesis genes (EBGs) such as chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), and flavonoid 3#-hydroxylase (F3#H), which are common to different flavonoid subpathways, are induced prior to late biosynthesis genes (LBGs) such as dihydroflavonol 4-reductase (DFR), leucoanthocyanidin oxygenase (LDOX), anthocyanidin reductase (ANR), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UF3GT; Pelletier et al., 1997). EBGs and LBGs are transcriptionally activated by R2R3-MYBs, such as PRODUCTION OF ANTHOCYANIN PIG-MENT1 (PAP1) a...

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“…The data presented here suggest that, in nonsenescing leaves, ethylene plays a different role and the inability to perceive ethylene results in reduced Rubisco expression and photosynthetic capacity. Interestingly, it has been shown that external sugar application results in significant stimulation of ethylene production in rice (Kobayashi and Saka, 2000;Seneweera et al, 2003). Seneweera et al (2003) suggested that ethylene production may promote growth under circumstances where leaf Glc concentrations are high, such as in plants growing in elevated atmospheric CO 2 levels.…”

Section: Ethylene As a Regulating Factor In Growth And Photosynthesis?mentioning

confidence: 99%

Ethylene Insensitivity Results in Down-Regulation of Rubisco Expression and Photosynthetic Capacity in Tobacco

et al. 2007

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Little is known about the effect of hormones on the photosynthetic process. Therefore, we studied Rubisco content and expression along with gas exchange parameters in transgenic tobacco (Nicotiana tabacum) plants that are not able to sense ethylene. We also tested for a possible interaction between ethylene insensitivity, abscisic acid (ABA), and sugar feedback on photosynthesis. We measured Rubisco content in seedlings grown in agar with or without added sugar and fluridone, and Rubisco expression in hydroponically grown vegetative plants grown at low and high CO 2 . Furthermore, we analyzed gas exchange and the photosynthetic machinery of transformants and wild-type plants grown under standard conditions. In the presence of exogenous glucose (Glc), agar-grown seedlings of the ethylene-insensitive genotype had lower amounts of Rubisco per unit leaf area than the wild type. No differences in Rubisco content were found between ethylene-insensitive and wild-type seedlings treated with fluridone, suggesting that inhibition of ABA production nullified the effect of Glc application. When larger, vegetative plants were grown at different atmospheric CO 2 concentrations, a negative correlation was found between Glc concentration in the leaves and Rubisco gene expression, with stronger repression by high Glc concentrations in ethyleneinsensitive plants. Ethylene insensitivity resulted in plants with comparable fractions of nitrogen invested in light harvesting, but lower amounts in electron transport and Rubisco. Consequently, photosynthetic capacity of the insensitive genotype was clearly lower compared with the wild type. We conclude that the inability to perceive ethylene results in increased sensitivity to Glc, which may be mediated by a higher ABA concentration. This increased sensitivity to endogenous Glc has negative consequences for Rubisco content and photosynthetic capacity of these plants.

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Relationship between Ethylene Evolution and Sucrose Content in Excised Leaf Blades of Rice

Cited by 20 publications

References 26 publications

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Ethylene Suppression of Sugar-Induced Anthocyanin Pigmentation in Arabidopsis

Ethylene Insensitivity Results in Down-Regulation of Rubisco Expression and Photosynthetic Capacity in Tobacco

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