Relationship between debris within grape bunches and &lt;i&gt;Botrytis&lt;/i&gt; infection of berries

Seyb, A. M.; Gaunt, R. E.; Trought, Michael C. T.; Frampton, Chris; Balasubramaniam, R.; Jaspers, Marlene V.

doi:10.30843/nzpp.2000.53.3682

Cited by 7 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the early season, conidia infect flowers through the styles, ovules, stamens, or petals, and infect young berries via the pedicel [4]. These infections cause blossom blight and the saprophytic colonization of "bunch trash" consisting of calyptras, tendrils, dead stamens, aborted flowers and berries [4,6]. Under favorable conditions, the saprophytic mycelium produces abundant conidia on the colonized bunch trash, which is considered a major source of inoculum for late-season infections [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Use of LAMP for Assessing Botrytis cinerea Colonization of Bunch Trash and Latent Infection of Berries in Grapevines

Ammour

Castaldo²,

Fedele

et al. 2020

Plants

View full text Add to dashboard Cite

A real-time loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Botrytis cinerea in grapevine bunch trash, immature berries, and ripening berries. A simple method for the preparation of crude extracts of grape tissue was also developed for on-site LAMP analysis. When tested with 14 other fungal species frequently found in grapevines, the LAMP assay was specific and sensitive to a B. cinerea DNA quantity of 0.1 ng/µL. The sensitivity was further tested using bunch trash samples with B. cinerea colonization levels between 6 and 100% and with bulk-berry samples composed of 4 pathogen-free berries or 4 berries among which 25 to 100% had been inoculated with B. cinerea. The LAMP assay detected the lowest B. cinerea colonization level tested in bunch trash and in immature and mature berries in less than 20 min. In single-berry experiments, LAMP amplified B. cinerea DNA from all artificially inoculated individual immature and mature berries. No amplification occurred in B. cinerea-free material. The real-time LAMP assay has the potential to be used as a rapid on-site diagnostic tool for assessing B. cinerea colonization in bunch trash and B. cinerea latent infections in berries, which represent critical stages for decision-making about disease management.

show abstract

Section: Introductionmentioning

confidence: 99%

Use of LAMP for Assessing Botrytis cinerea Colonization of Bunch Trash and Latent Infection of Berries in Grapevines

Ammour

Castaldo²,

Fedele

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…cinerea has a complex lifecycle and attacks grapevines via multiple infection pathways; some of the pathways occur early in the season (i.e., from flowering to young cluster development) (Elmer and Michailides 2007). One early season pathway involves the saprophytic colonization of bunch trash; this colonization has been traditionally considered a major source of inoculum within developing bunches (Nair and Hill 1992;Nair and Parker 1985), and correlations between bunch trash colonization by B. cinerea and BBR incidence at harvest have been reported (Seyb et al 2000). As a consequence, B. cinerea chemical control in the early season and especially, at prebunch closure has been recommended (Corvi and Tullio 1980;Pérez-Marín 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The later season applications (i.e., the applications from veraison until harvest; C and D) are aimed not only at preventing berry infection during ripening but also, at disinfesting bunch trash to reduce the inoculum load (Baldacci et al 1962;Calvo-Garrido et al 2014a). Bunch trash colonized by B. cinerea is, therefore, considered an important source of inoculum for infection from flowering until ripening (Calvo-Garrido et al 2014a;Holz et al 2003;Nair et al 1995;Viret et al 2004), and the incidence of B. cinerea in bunch trash is associated with the severity of BBR at harvest (Keller et al 2003;McClellan and Hewitt 1973;Seyb et al 2000).…”

mentioning

confidence: 99%

Reduction of Botrytis cinerea Colonization of and Sporulation on Bunch Trash

et al. 2020

View full text Add to dashboard Cite

Botrytis bunch rot (BBR) of grapevine, caused by Botrytis cinerea, is commonly managed by fungicide (FUN) sprays at flowering (A), at prebunch closure (B), at veraison (C), and before harvest. Applications at A, B, and C are recommended to reduce B. cinerea colonization of bunch trash and the production of conidia during berry ripening. The effects of these applications were previously evaluated as reductions in BBR severity at harvest rather than as reductions in bunch trash colonization and sporulation by B. cinerea. This study investigated the effects of FUNs (a commercial mixture of fludioxonil and cyprodonil), biological control agents (BCAs; Aureobasium pullulans and Trichoderma atroviride), and botanicals (BOTs; a commercial mixture of eugenol, geraniol, and thymol) applied at different timings (A, B, C, or ABC) compared with a nontreated control (NT) on B. cinerea bunch trash colonization and sporulation in vineyards. The ability of B. cinerea to colonize the bunch trash (as indicated by B. cinerea DNA content) and sporulate (as indicated by the number of conidia produced under optimal laboratory conditions) was highly variable, and this variability was higher between years (2015 to 2018) than among the three vineyards and three sampling times (i.e., 1 week after applications at A, B, and C). B. cinerea sporulation on bunch trash was significantly lower in plots treated with FUN than in NT in only 3 of 18 cases (3 vineyards × 2 years × 3 sampling times). FUN applications, however, significantly reduced B. cinerea colonization of bunch trash compared with NT; for colonization, BCA efficacy was similar to that of FUN, but BOT efficacy was variable. For all products, colonization reduction was the same with application at A versus ABC, meaning that the effect of an early season application lasted from flowering to 1 week after veraison. These results indicate that the early season control of B. cinerea is important to reduce the saprophytic colonization of bunch trash, especially when the risk of BBR is high.

show abstract

“…Grapevine bunch trash colonized by B. cinerea has been considered an important source of inoculum for infection from flowering to ripening [46][47][48][49] and has been related to the severity of BBR at harvest [50][51][52]. Thus, the reduction in B. cinerea inoculum on bunch trash from flowering to pre-bunch closure may contribute to BBR control, as shown in a meta-analysis of 116 studies [53,54] and in several field experiments [12,13,55].…”

Section: Discussionmentioning

confidence: 99%

“…To understand how to reduce this B. cinerea inoculum, researchers have studied the effects of removing the bunch trash from clusters at fruit set by the use of compressed air or leaf blowers [56], or by the application of fungicides [52] between the end of flowering (A) and pre-bunch closure (B); pre-bunch closure is considered the last stage for disinfesting the bunch trash inside the cluster because subsequent expansion of berries impedes the deposition of fungicides on the bunch trash [52,57]. BCAs have also been studied as alternatives for reducing the colonization of bunch trash by B. cinerea [12,13,58].…”

Section: Discussionmentioning

confidence: 99%

The Colonization of Grape Bunch Trash by Microorganisms for the Biocontrol of Botrytis cinerea as Influenced by Temperature and Humidity

et al. 2020

View full text Add to dashboard Cite

Six commercial biocontrol agents (BCAs: Aureobasidium pullulans, Bacillus amyloliquefaciens, B. amyloliquefaciens plantarum, B. subtilis, Metschnikowia fructicola, or Trichoderma atroviride) were applied to bunch trash that was then incubated at one of five temperatures (T, 15, 20, 25, 30, and 35 °C) and one of five relative humidity levels (RH, 60, 80, 90, 95, and 100%). After 1 to 13 days of incubation (BCA colonization period), the number of colony forming units (CFUs) was assessed. The colonization of bunch trash in response to T/RH conditions and BCA colonization period differed among the BCAs; the coefficients of variation among the BCAs ranged from 104.6 to 397.7%. Equations were developed that accounted for the combined effects of the T, RH, and BCA colonization period on BCA colonization of bunch trash. Assuming that the equations, which had an R2 > 0.87, correctly predict BCA growth under field conditions, they would help farmers select the BCA to be used for a specific application based on weather conditions at the time of treatment and in the following days. The equations would also help predict how long an early season BCA application remains effective and thereby help farmers decide whether and when a second BCA application may be needed.

show abstract

Relationship between debris within grape bunches and <i>Botrytis</i> infection of berries

Cited by 7 publications

References 0 publications

Use of LAMP for Assessing Botrytis cinerea Colonization of Bunch Trash and Latent Infection of Berries in Grapevines

Use of LAMP for Assessing Botrytis cinerea Colonization of Bunch Trash and Latent Infection of Berries in Grapevines

Reduction of Botrytis cinerea Colonization of and Sporulation on Bunch Trash

The Colonization of Grape Bunch Trash by Microorganisms for the Biocontrol of Botrytis cinerea as Influenced by Temperature and Humidity

Contact Info

Product

Resources

About