Relationship between CT signs and the expression of miR‑146a in colon cancer

Abstract

Our study investigated the relationship between CT signs of colon cancer and the expression of miR-146a, to evaluate their predictive value in early diagnosis and of colon cancer. A total of 216 patients with colon cancer who were admitted to the Department of Hematology and Oncology in Yingcheng Hospital, the Second Hospital of Shandong University in Zhaoyuan City from January 2013 to March 2017 were selected as the study group. At the same time, 150 patients with benign colitis were also included to serve as the control group. CT scan was performed for patients in both the study and control groups. The expression of miR-146a in serum of patients in the study and control groups was detected by RT-qPCR, and of CT signs and miR-146a expression were analyzed. The relative expression level of miR-146a in peripheral blood of study group was significantly lower than that of the control group (t=12.180, p=0.001). Sensitivity and specificity of combined detection of CT and miR-146a in the study and control groups were 91.4 and 93.6%, respectively, which were higher than those of the single test (p<0.05). Among CT signs of study group, luminal wall, cavity and diameter changes, as well as size of tumor and surrounding tissues showed no significant correlation with the expression of miR-146a (p>0.05). The expression level of miR-146a in patients with high-risk of invasion was significantly higher than that in patients with low-risk of invasion (t=4.791, p=0.001). The expression level of miR-146a in lymph node metastasis was significantly lower than that in non-lymph node metastasis (t=3.104, p=0.002). miR-146a may be involved in the development and progression of colon cancer. Combined detection of CT and miR-146a may enhance the sensitivity and specificity of the diagnosis of colon cancer, and may provide a good predictive value for invasion and metastasis of colon cancer.

GeromiRs Are Downregulated in the Tumor Microenvironment during Colon Cancer Colonization of the Liver in a Murine Metastasis Model

“…We observed that the most statistically significant miRNA downregulated in the TME cells, miR-146a, was a geromiR ( Figure 5 A). Liu et al [ 22 ] found that the relative expression level of miR-146a in the peripheral blood of colon cancer patients was significantly lower than that of a control group with benign colitis. Simanovich et al [ 23 ] showed that miR-146a-5p functions as a control switch between angiogenesis and cell death, its neutralization can manipulate the crosstalk between tumor cells and macrophages, and profoundly change the TME.…”

Lack of association betweenmiR-146ars2910164 C/G locus and colorectal cancer: from a case–control study to a meta-analysis

“…In CRC patients, miR-146a expression level decreased in progressed tumors [ 9 ]. Since miR-146a plays important roles in the development of CRC, we presumed that miR-146a rs2910164 variants could influence the susceptibility to CRC.…”

“…Adami et al indicated that miR-146a was overexpressed in primary gastric tumor tissues without the status of chronic Helicobacter pylori infection and expressed a related lower level in progressed gastric cancer [ 8 ]. In addition, a recent study reported that miR-146a expression level decreased in progressed CRC patients with lymph node metastasis [ 9 ]. Thus, miR-146a was considered to be implicated in the development and progression of cancer [ 10 , 11 ].…”

The diagnostic value of miR-21 combined with CT in patients with liver cancer

“…However, many studies have shown the value of serum markers combined with CT diagnosis. The studies by Liu et al [30] showed that miR-146a combined with CT can significantly improve the diagnostic efficiency of colon cancer, and can provide a great prediction value for invasion and metastasis of colon cancer. He et al [31] and others have proposed that the use of miRNA can improve CT diagnosis of lung cancer and pulmonary nodules and greatly reduce false positives diagnosis.…”