Relationship between cold hardiness and dehydrin gene expression in peach shoot tissues under field conditions

Abstract

The relationship between cold hardiness and expression of dehydrin genes in the shoots of four peach cultivars (Prunus persica 'Daewol', 'Aikawanakajima', 'Fukuyokabijin', and 'Kiraranokiwami') was quantitatively evaluated during seasonal cold acclimation and deacclimation. Cold hardiness was determined by electrolyte leakage analysis, which increased gradually during fall, reached a maximum level in midwinter, and decreased again in spring. According to the temperatures at which 50% injury occurred (LT50), differences in cold hardiness of 'Daewol' and 'Kiraranokiwami' were the most obvious during deacclimation, with the exception of April 2012. The expression patterns of PpDhn1 and PpDhn3 showed parallel fluctuations in cold hardiness in the four cultivars, whereas PpDhn2 did not show any seasonal changes in any of the cultivars.

Proline accumulation in response to high temperature in winter-acclimated shoots ofPrunus persica: a response associated with growth resumption or heat stress?

“…One-year-old 'Daewol' peach trees, commercially grafted on wild peach seedlings, were used in this study. This cultivar was selected because in our previous studies (Shin et al 2015a(Shin et al , 2015b(Shin et al , 2015c, 'Daewol' shoots showed the most sensitive response to seasonal or abrupt changes in temperatures (transition from coldhardened to dehardened status), with accompanying changes in soluble sugars, dehydrins, and related gene expression. On 2 Feb. 2015, a total of 35 one-year-old trees were dug up from the nursery and planted in 2-L pots containing sandy loam soil and maintained outdoors (latitude 36°43′07.2″N, longitude 127°29′00.0″E) for 4 d (average temperature 0.6°C; max.…”

Dehydrins and Soluble Sugars Involved in Cold Acclimation of Rosa wichurana and Rose Cultivar ‘Yesterday’

“…From February to April, the decrease of RhDHN5 and RhDHN6 was associated with deacclimation; this correlation has also been observed in blueberry and Scots pine [48,49]. The seasonal pattern of dehydrin genes/proteins is also reported in different tissues (leaves, stem, and floral buds) of woody plants (e.g., apple, peach, birch, Rhododendron) during the overwintering process [50][51][52][53][54]. The observed seasonal dynamic of dehydrins in rose showed a positive relation to the changing pattern of cold hardiness level (determined by LT 50 ), suggesting that dehydrins might be closely associated with the freezing tolerance of rose.…”

Ethylene-Mediated Modulation of Bud Phenology, Cold Hardiness, and Hormone Biosynthesis in Peach (Prunus persica)

“…This loss of cold hardiness, or deacclimation is a function of many external and internal factors, such as ambient temperatures, photoperiod, water availability, metabolism status, and dormancy status [ 48 ]. Change in cold hardiness in peach is closely related to the soluble sugar content and the accumulation of a 60 kDa dehydrin protein [ 49 ]. Our results indicate that cold hardiness is highly dependent on the depth of dormancy and was improved by ethephon applications ( Figure 2 B), which is generally consistent with other reports on grapevine [ 50 ], peach [ 6 ], and magnolia ( Magnolia wufengensis ) [ 51 ].…”