Relationship between Cell Permeability and Respiration in Ripening Banana Fruit Tissue

Baur, J. R.; Workman, M.

doi:10.1104/pp.39.4.540

Cited by 25 publications

(11 citation statements)

References 3 publications

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“…Although the increases in iso-amyl acetate concentration were believed to result from its production by the slices during incubation, the poss'ibility existed that they reflected gradual equilibration of the incubation mixtures. Unripe slices are hard and have 'a high degree of cellular integrity which decreases as ripening progresses (Sacher, 1962;Bain et al, 1964;Bauer et al, 1964). Hence, equilibration of the vapor composition over unripe slices might require more time than for ripe slices.…”

Section: Resultsmentioning

confidence: 99%

Vapor Analysis of the Production by Banana Fruit of Certain Volatile Constituents

Myers

Issenberg

Wick

1969

Journal of Food Science

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SUMMARY: Direct gas chromatographic vapor analyses were utilized to determine whether banana slices at different stages of ripeness in an in vitro system produced iso‐amyl acetate and iso‐amyl alcohol, known banana aroma constituents; and whether a precursor‐product relationship could be observed between these compounds and L‐leucine. Production of iso‐amyl acetate by unripe slices was demonstrated based on experiments with metabolic inhibitors. The behavior of ripe and overripe slices was inconclusive since the vapor concentration of the acetate remained constant. The vapor concentration of iso‐amyl alcohol was essentially unchanged at all ripeness stages. Investigations with L‐leucine‐U‐14C showed conclusively that leucine was a precursor and that both compounds were continually produced at all ripeness stages. The interpretation of direct vapor analyses over respiring fruit is considered.

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Section: Resultsmentioning

confidence: 99%

Vapor Analysis of the Production by Banana Fruit of Certain Volatile Constituents

Myers

Issenberg

Wick

1969

Journal of Food Science

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“…They found that changes in the inner structure of the chloroplasts had occurred by the time the climacteric has begun. Other workers (Rhodes & Wooltorton, 1967) have found disorganization of structure during the climacteric, and Sacher (1966) and Bauer & Workman (1964) concluded that permeability changes may be considered to be the cause of the climacteric, a view endorsed by Young & Biale (1967) in a study of the uptake of 32P by disks of avocado pears.…”

Section: Initiation Of Ethylene Productionmentioning

confidence: 94%

Biogenesis of Ethylene

Mapson¹

1969

Biological Reviews

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Summary 1. The main characteristics of the biosynthetic system forming ethylene in plant tissues have been reviewed. The dependence of synthesis on a liberal supply of oxygen is clearly indicated by the fact that atmospheres containing 3–5% oxygen prevent the synthesis in fruits. There is no close connexion between respiratory activity and synthesis. Ripening of fruits and the changes associated with it may be initiated by ethylene; under such conditions the progress of formation of the hydrocarbon is autocatalytic. 2. Synthesis appears to be dependent on some degree of cell organization, since it responds acutely to changes in toxcity, tissue wounding and tissue destruction. Homogenates of many plant tissues do not produce ethylene in vitro, and the inability to use such extracts has imposed serious restrictions on biochemical studies which have in the past been mainly concerned with tracer studies and the use of tissue slices. 3. The chief difficulty associated with tracer studies aimed at determining the nature of the precursor stems from the fact that the synthesis of ethylene is only a minor pathway on the general metabolism of the cell. Thus the ratio of CO2 to ethylene production is of the order of 164 in the case of the apple and as high as 18,000 in the case of less vigorous producers of ethylene. The incorporation of label from labelled substrates which enter the general metabolism of the cell is thus usually very low, and this makes it difficult to determine whether the incorporation observed has any real physiological significance. In fact only where incorporation into ethylene relative to that into CO2 is high, as is the case with methionine, can one conclude that the substance can be considered to be an immediate precursor. 4. Because of the difficulty of obtaining clear‐cut results with tracer techniques, attention has been devoted to the production of ethylene by model systems from substances of physiological interest. The studies have revealed that many substances found in plant tissue can be decomposed to yield ethylene in model systems functioning under physiological conditions. Two such substances, which have received most attention, are methionine and linolenic acid, and conditions under which ethylene is formed from them have been described. 5. Such developments have stimulated research to obtain evidence for or against the operation of such model systems in vivo. Using tissue‐slice techniques, methionine and linolenic acid have both been found to stimulate ethylene formation in tissue slices. 6. The first demonstration of the synthesis of ethylene in vitro by enzymes isolated from the florets of the cauliflower has now been reported. The system involves the intermediate formation of methional from methionine by enzymes contained in the mitochondria, and the subsequent enzymic decomposition of methional into ethylene by non‐particulate enzymes. These latter consist of a glucose oxidase and a peroxidase. The glucose oxidase in the presence of its substrate generates hydrogen peroxide, and per...

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“…Consideration of the changes induced in steles by aging seems to be irrelevant; it is certain that these are the effects of advancing necrosis and have no relevance to the function of the stele in the intact root. Indeed, if the reason given by Hall et al (42) for the enhancement of respiration rate in aged ste1es, where they liken it to the climacteric shown in aged fruits and thought to be "associated with, or a result of, increased membrane permeabil ity (14)," then the enhancement of Cl-uptake with aging must also be seen as the result of an increase in "leakiness" with aging rather than the reverse.…”

Section: Ion Transport In Various Plant Tissuesmentioning

confidence: 97%

“…The authors rightly reject any comparison with the aging effects in storage tissue, although both aged steles and aged storage tissue discs show enhanced respiration rates and ion uptake rates. They consider the effect in steles to be comparable to the climacteric in respiration found in fruits at the onset of ripening (14). Thus aging in storage tissue is in a sense a break in dormancy; aging in steles is the onset of necrosis.…”

Section: Ion Transport In Various Plant Tissuesmentioning

confidence: 98%

Ion Transport in the Cells of Higher Plant Tissues

Anderson

1972

Annu. Rev. Plant. Physiol.

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Relationship between Cell Permeability and Respiration in Ripening Banana Fruit Tissue

Cited by 25 publications

References 3 publications

Vapor Analysis of the Production by Banana Fruit of Certain Volatile Constituents

Vapor Analysis of the Production by Banana Fruit of Certain Volatile Constituents

Biogenesis of Ethylene

Ion Transport in the Cells of Higher Plant Tissues

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