Relationship between CD14-159C/T gene polymorphism and acute brucellosis risk

Moghadampour, Mehdi; Eskandari-Nasab, Ebrahim; Shabani, Fatemeh

doi:10.1016/j.apjtm.2016.01.036

Cited by 6 publications

(4 citation statements)

References 39 publications

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“… 14 Genetic variants of the CD14 gene (a C-to-T transition, snp −159 C/T) have been shown to be associated with susceptibility to multiple diseases and conditions including allergic rhinitis, 15 functional dyspepsia, 16 inflammatory bowel disease, 17 gram-negative bacteria, and respiratory infections, including brucellosis. 18 – 21 Its significance in allergic reactions and outcome in patients with asthma have also been documented. 21 , 22 – 24 In human, malaria infections particularly, elevated levels of soluble CD14 have been found in patients with acute infections, 25 concluding that soluble CD14 levels may play a critical role in disease severity.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of CD14 Promoter Gene Polymorphism (rs2569190) is Associated With Regulation of Malaria Parasitemia and Susceptibility to Plasmodium falciparum Infection

et al. 2017

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CD14 is a multifunctional receptor expressed on many cell types and has been shown to mediate immune response resulting in the activation of an inflammatory cascade, with polymorphism of its promoter (rs2569190) found to be associated with susceptibility to several diseases. In malaria infection, the CD14 gene demonstrated a pathogenic profile in regulating experimental cerebral malaria, with reports of elevated levels of soluble CD14 in serum of patients but no definitive conclusion. We present a detailed analysis of genetic diversity of CD14 promoter gene (snp −159 C/T; rs2519190) polymorphism between a malaria-infected group and uninfected controls and its association with clinical parameters of disease. Genomic DNA samples obtained from 106 Plasmodium falciparum malaria–infected patients and 277 uninfected controls were elucidated with a polymerase chain reaction-restriction fragment length polymorphism (RFLP) assay. Our results show a significant diversity (P = 3.32E−06) in the genotypic frequency (3.8% versus 22.4%) of the rs2569190 mutant variant between the malaria-infected group and controls, respectively. The mutant allele had the lowest frequency among the malaria-infected group demonstrating its necessity for infection. Mean parasitemia (parasites/μL of blood) was significantly regulated based on CD14 polymorphic profile (19 855 versus 37 041 versus 49 396 for homozygote mutants, heterozygotes, and homozygote wild type, respectively). Interestingly, we found no association between CD14 genetic variants with fever, age of patients, or anemia. How this affects disease severity between subregional and continental groups deserves further clarification, including extending these studies in a larger group and among severe and asymptomatic patients with malaria.

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Section: Introductionmentioning

confidence: 99%

Genetic Diversity of CD14 Promoter Gene Polymorphism (rs2569190) is Associated With Regulation of Malaria Parasitemia and Susceptibility to Plasmodium falciparum Infection

et al. 2017

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“…is the most important virulence factor of Brucella spp., affecting entry into phagocytic cells and protecting from the immune response. 10 While cell-mediated immunity is the major mechanism for resistance to brucellosis, acquired humoral immunity also plays an important role in recovery from the disease. 1 Hypersensitivity to Brucella antigens develops synchronously with immune response, resulting in the formation of granulomas that may contribute to some of the clinical manifestations of the disease; these granulomas may participate in restraining bacterial growth as an antibacterial defense mechanism of macrophages and lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

“…It was found that the prevalence of CD14-159 TT and CT genotypes were correlated with an increased risk of brucellosis. 10 A study that questioned association of IL-17 (inflammatory cytokine, which acts as a regulator reduces releasing of interferon-gamma [IFN-γ] and increases resistance to brucellosis) gene polymorphisms and susceptibility to brucellosis, was found that the GG genotype at positions rs3748067, rs3819025, rs4711998, and AA genotype at positions rs3819024 were correlated with an increased risk against the disease. 13 IFN-γ is known as one of the main cytokines which play an important role in immunity against Brucella spp.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D Receptor Gene Polymorphisms in Childhood Brucellosis in Turkish Children

Kaman

Öz

Akcan

2021

J Pediatr Infect Dis

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Objective The vitamin D receptor gene (VDR) polymorphisms and the risk of various infections have been studied. An association with brucellosis and vitamin D levels has been investigated but not yet with VDR gene polymorphisms. We aimed to examine the association between VDR gene polymorphisms and susceptibility to childhood brucellosis. Methods This case–control study included patients with brucellosis and healthy controls. After extracting genomic DNA using a Qiagen blood DNA isolation kit, five VDR single nucleotide polymorphisms (SNPs), including Cdx-2, FokI, BsmI, ApaI, and TaqI gene, were amplified. Genetic distribution of these SNPs of VDR gene in patient and control groups were compared. Results A total of 38 patients with brucellosis and 89 healthy controls were evaluated. The genotype distribution of Cdx2, FokI, BsmI, and ApaI polymorphisms were similar between patients and healthy controls. However, the CC homozygous genotype for VDR gene TaqI was significantly overexpressed in patients compared with controls (23.7 vs. 7.9%; p = 0.042). The frequency of the C allele of the TaqI genotype was significantly different between patients and controls (p = 0.018). On the other hand, presence of the A allele in the BsmI was associated considerably with an increased risk of brucellosis (p = 0.037). VDR polymorphism distribution was similar according to age, presence of complicated disease, and presence of bacteremia. The heterozygote TaqI polymorphism was more common in patients presented as subacute and chronic symptoms (p = 0.036). Conclusion Our results indicated the possible role in TaqI polymorphism of the VDR gene for the risk of brucellosis at the time of exposure to infection.

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“…3 Patients with active brucellosis have symptoms such as fever, headache, sweating, weakness, weight loss, persistent joint pain, endocarditis, neurological complications, and testicular or bone abscess formation. 4,5 Brucella spp. invade reticuloendothelial system cells and can reproduce in these cells, and escape from the host's immune system.…”

Section: Introductionmentioning

confidence: 99%

The relationship between IFN-γ and TNF-α gene polymorphisms and brucellosis: Meta-analysis

Eskandari-Nasab¹,

Moghadampour²

2018

Adv Clin Exp Med

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Relationship between CD14-159C/T gene polymorphism and acute brucellosis risk

Abstract: The findings provided suggestive evidence of association of the CD14-159C/T gene polymorphism with susceptibility to acute brucellosis in the Iranian population.

Cited by 6 publications

References 39 publications

Genetic Diversity of CD14 Promoter Gene Polymorphism (rs2569190) is Associated With Regulation of Malaria Parasitemia and Susceptibility to Plasmodium falciparum Infection

Genetic Diversity of CD14 Promoter Gene Polymorphism (rs2569190) is Associated With Regulation of Malaria Parasitemia and Susceptibility to Plasmodium falciparum Infection

Vitamin D Receptor Gene Polymorphisms in Childhood Brucellosis in Turkish Children

The relationship between IFN-γ and TNF-α gene polymorphisms and brucellosis: Meta-analysis

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