Relationship between bovine oocyte morphology and <i>in vitro</i> developmental potential

Nagano, Masashi; Katagiri, Seiji; Takahashi, Yoshiyuki

doi:10.1017/s0967199406003510

Cited by 84 publications

(95 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is not too different from the requirements of the glucose-deprived cleavage stage embryo that must rely on accelerated uptake of monocarboxylates or perhaps exogenous oocyte-derived lipid. There is evidence in species where oocytes are more lipid loaded than mice, of lipid metabolism being related to developmental potential (Zeron et al 2001, Boni et al 2002, Nagano et al 2006. The analogy between the embryo and ES cells is reinforced by the observation that PPARA activation leads to increased intracellular ROS.…”

Section: Metabolic Stress In Embryosmentioning

confidence: 99%

Glucose deprivation, oxidative stress and peroxisome proliferator-activated receptor-α (PPARA) cause peroxisome proliferation in preimplantation mouse embryos

Jansen

Cashman²,

Thompson³

et al. 2009

Reproduction

View full text Add to dashboard Cite

Ex vivo two-cell mouse embryos deprived of glucose in vitro can develop to blastocysts by increasing their pyruvate consumption; however, zygotes when glucose-deprived cannot adapt this metabolic profile and degenerate as morulae. Prior to their death, these glucose-deprived morulae exhibit upregulation of the H C -monocarboxylate co-transporter SLC16A7 and catalase, which partly co-localize in peroxisomes. SLC16A7 has been linked to redox shuttling for peroxisomal b-oxidation. Peroxisomal function is unclear during preimplantation development, but as a peroxisomal transporter in embryos, SLC16A7 may be involved and influenced by peroxisome proliferators such as peroxisome proliferator-activated receptor-a (PPARA). PCR confirmed Ppara mRNA expression in mouse embryos. Zygotes were cultured with or without glucose and with the PPARA-selective agonist WY14643 and the developing embryos assessed for expression of PPARA and phospho-PPARA in relation to the upregulation of SLC16A7 and catalase driven by glucose deprivation, indicative of peroxisomal proliferation. Reactive oxygen species (ROS) production and relationship to PPARA expression were also analysed. In glucose-deprived zygotes, ROS was elevated within 2 h, as were PPARA expression within 8 h and catalase and SLC16A7 after 12-24 h compared with glucose-supplied embryos. Inhibition of ROS production prevented this induction of PPARA and SLC16A7. Selective PPARA agonism with WY14643 also induced SLC16A7 and catalase expression in the presence of glucose. These data suggest that glucose-deprived cleavage stage embryos, although supplied with sufficient monocarboxylate-derived energy, undergo oxidative stress and exhibit elevated ROS, which in turn upregulates PPARA, catalase and SLC16A7 in a classical peroxisomal proliferation response.

show abstract

Section: Metabolic Stress In Embryosmentioning

confidence: 99%

Glucose deprivation, oxidative stress and peroxisome proliferator-activated receptor-α (PPARA) cause peroxisome proliferation in preimplantation mouse embryos

Jansen

Cashman²,

Thompson³

et al. 2009

Reproduction

View full text Add to dashboard Cite

show abstract

“…They were then fixed with a mixture of ethanol: acetic acid (3:1), stained with 1% aceto-orcein solution, and nuclear status and the presence of pronuclei were evaluated using a phase-contrast microscope, as described previously [22,23]. Briefly, the presence of two pronuclei was regarded as normal fertilization, whereas three or more pronuclei and/or enlarged sperm heads within an oocyte was judged as polyspermy.…”

Section: Evaluation Of Nuclear Maturation and Fertilization By Whole-mentioning

confidence: 99%

Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

et al. 2008

View full text Add to dashboard Cite

This study was conducted to improve in vitro production of embryos from domestic cats using TCM-199 as an IVM medium. The time sequence of nuclear maturation and the optimal timing of in vitro insemination were examined. Most oocytes were at the germinal vesicle stage immediately after collection; however, 8.3% had already resumed meiosis before IVM culture. After 30 h of IVM culture, the percentage of oocytes at metaphase II (MII) reached a peak (75.5%) and did not change (P>0.05) from 30 to 48 h after IVM culture. The percentage of oocytes with two pronuclei was higher (P<0.05) for oocytes matured for 30 and 36 h (38.2 and 33.0%, respectively) than for those after IVM culture for only 24 h (18.5%).Total sperm penetration rate was highest (P<0.05) for oocytes that had been matured for 30 h (46.1%). After 30 h of IVM and 18 h of IVF culture, 66.3 and 24.8% of inseminated oocytes had cleaved and developed to the blastocyst stage, respectively. We concluded that IVM of feline oocytes for 30 h in TCM-199 resulted in optimal nuclear maturation and sperm penetration.

show abstract

“…Finally, we discuss the relationship between the ATP level and developmental ability of oocytes at metaphase II (MII) using our previous results (Nagano et al, 2006).…”

Section: Introductionmentioning

confidence: 96%

ATP content and maturational/developmental ability of bovine oocytes with various cytoplasmic morphologies

Nagano

Katagiri

Takahashi

2006

Zygote

Self Cite

View full text Add to dashboard Cite

SummaryWe examined the relationship among morphological appearance (six groups) of bovine oocytes, ATP content and maturational/developmental ability. Oocytes with a brown ooplasm (with or without a dark region) had intermediate levels of ATP at the germinal vesicle (GV) stage and showed higher rates of first polar body (PB) extrusion than the other groups. Oocytes with a low level of ATP (oocytes with a pale ooplasm without dark clusters) and oocytes with a high level of ATP (oocytes with a black ooplasm) showed lower rates of PB extrusion. During in vitro maturation, ATP levels in oocytes decreased at around GV breakdown and increased toward metaphase II (MII). MII oocytes having a brown ooplasm with a dark region, which had good developmental capacity, had a relatively high level of ATP. MII oocytes with a brown or pale ooplasm without dark clusters, which had poor developmental capacity, had low ATP levels. MII oocytes with a black ooplasm, which had poor developmental capacity, had an unusually high level of ATP. These results suggest that the morphological appearance of bovine oocytes is closely related to their ATP levels and that cytoplasmic morphology will give an advantage for the selection of oocytes with a high maturational and developmental ability.

show abstract

Relationship between bovine oocyte morphology and in vitro developmental potential

Cited by 84 publications

References 35 publications

Glucose deprivation, oxidative stress and peroxisome proliferator-activated receptor-α (PPARA) cause peroxisome proliferation in preimplantation mouse embryos

Glucose deprivation, oxidative stress and peroxisome proliferator-activated receptor-α (PPARA) cause peroxisome proliferation in preimplantation mouse embryos

Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

ATP content and maturational/developmental ability of bovine oocytes with various cytoplasmic morphologies

Contact Info

Product

Resources

About