Relationship Between Bicarbonate Retention and Bone Characteristics in Broiler Chickens

Abstract

Determination of the bicarbonate retention factor (BRF) is an important step during development of the indicator amino acid oxidation technique for use in a new model. A series of 4-h oxidation experiments were performed to determine the BRF of broilers aged 7, 14, 21, 28, 35, and 42 d using 4 birds per age group. A priming dose of 1.2 microCi of NaH(14)CO(3), followed by eight half-hourly doses of 1 microCi of NaH(14)CO(3) were given orally to each of 4 birds per age. The percentage of (14)C dose expired by the bird at a steady state was measured. These birds, as well as 12 additional birds matched for age and BW, were killed, and femur bone mineral density was measured by quantitative computed tomography to determine the relationship between bone development and bicarbonate retention at each age. There was a correlation (r = 0.50; P < 0.05) between total cross-sectional femur bone mineral density and bicarbonate retention at each age. A prediction equation (Y = 6.95 x 10(-2)X - 3.51 x 10(-5)X(2) + 27.58; P < 0.0001, R(2) = 0.79) where Y = bicarbonate retention and X = BW was generated to predict Y as a function of X. Bicarbonate retention values peaked at 28 d, during the stage of the most rapid bone deposition and the highest growth rate. A constant BRF was found from 1,900 to 2,700 g of BW of 35.15 +/- 1.095% (mean +/- SEM). This retention factor will allow the accurate correction of oxidation of (14)C-labeled substrates in broilers of different ages and BW in future indicator amino acid oxidation studies.

Effect of dietary citric acid on the performance and mineral metabolism of broiler

“…Mineral matrix is the major component of the bone, so mineral density is considered to reflect the status of bone health. The relative values for total density, trabecular density and cortical density were consistent with the values reported by Leslie et al. (2006).…”

“…Mineral matrix is the major component of the bone, so mineral density is considered to reflect the status of bone health. The relative values for total density, trabecular density and cortical density were consistent with the values reported by Leslie et al (2006). A high positive correlation between bonebreaking strength and bone mineral density has been reported by many researchers (Frost and Roland, 1991;Orban et al, 1993;Rath et al, 1999Rath et al, , 2000Massé et al, 2003).…”

Carbon stable‐isotope tracking in breath for comparative studies of fuel use

“…This delay may be negligible in active, small-bodied animals (<1 kg) that have comparatively high mass-specific metabolic rates and thus more rapid HCO 3 turnover rates, but in large animals (>5 kg), endotherms in inactive states, or ectotherms, the half-life for HCO 3 turnover can be ß1 h. Additionally, some of the 13 C tracer can be lost to other carbon pools in the body via diversion of TCA cycle intermediates to nonoxidative fates, or by loss of 13 CO 2 from the circulating bicarbonate pool to other carbon stores, such as bone carbonate. 70,71 In resting humans, approximately 20% of the 13 C tracer is not recovered in exhaled CO 2 . 70 Thus, in such studies, a correction factor, sometimes termed a retention factor (RF), of ß0.8 is applied, so that the oxidation rate of the labeled substrate can be more accurately assessed.…”

Photoperiodic responses of broilers. III. Tibial breaking strength and ash content

“…These have included growth and age (e.g. Wise, 1970;Bond et al, 1991;Leterrier and Nys, 1992;Bruno et al, 2000;Rath et al, 2000;Applegate and Lilburn, 2002;Leslie et al, 2006), gender (e.g. Bond et al, 1991;Yalcin et al, 1998Yalcin et al, , 2001Rath et al, 1999), genotype (e.g.…”