Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film

Yamamoto, Sadaaki; Tanaka, Masaru; Sunami, Hiroshi; Arai, Keiko; Takayama, Aiko; Yamashita, Shohei; Morita, Yuka; Shimomura, Masatsugu

doi:10.1016/j.susc.2006.01.085

Cited by 53 publications

(23 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The authors also report that condensation of actin cytoskeleton occurs on all microstructured surfaces. Interestingly, Yamamoto and coworkers [8,9,16] found that porcine aortic endothelial cells (PAECs) adhere better to honeycomb (HC)-patterned films from poly (ε-caprolactone) (PCL) coated on the rims with fibronectin compared to flat substrates. The authors attribute this to enhanced integrin activation on the microstructured films.…”

Section: Resultsmentioning

confidence: 99%

“…Mechanical signals play a significant role in the signaling system regulating cell and tissue development as well as physiology. Surface stimuli encompass the viscoelasticity of the substrate, surface patterning, wettability, roughness, and porosity [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. So far, model substrates with defined pore geometry and distribution have rarely been used to obtain quantitative data on cell spreading and morphological details such as cytoskeleton development and elastic properties of the cell body [11,12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cell Adhesion to Ordered Pores: Consequences for Cellular Elasticity

Janshoff

Lorenz

Pietuch

et al. 2010

Journal of Adhesion Science and Technology

View full text Add to dashboard Cite

The adhesion of MDCK II cells to porous and non-porous silicon substrates has been investigated by means of fluorescence and atomic force microscopy. The MDCK II cell density and the average height of the cells were increased on porous silicon substrates with regular 1.2 µm pores as compared to flat, non-porous surfaces. In addition, we found a substantially reduced actin cytoskeleton within confluent cells cultured on the macroporous substrate compared to flat surfaces. The perturbation of the cytoskeleton relates to a significantly reduced expression of integrins on the porous area. The loss of stress fibers and cortical actin is accompanied by a dramatically reduced Young's modulus of 0.15 kPa compared to 6 kPa on flat surfaces as revealed by site-specific force-indentation experiments.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cell Adhesion to Ordered Pores: Consequences for Cellular Elasticity

Janshoff

Lorenz

Pietuch

et al. 2010

Journal of Adhesion Science and Technology

View full text Add to dashboard Cite

show abstract

“…[33][34][35] The adhesion and morphology of cells are influenced by variety and structure of proteins adsorbed on the material surface. [36][37][38][39] Fibronectin adsorb site-selectively on the honeycomb-patterned film plays vital role in cell adhesion, morphology and growth. 39,40) In this study, the adhesion and morphologies of the hepatocytes on the honeycomb-patterned films were clearly different from that on the flat film, suggesting that these differences are caused by site-selective adsorption of cell adhesion proteins like fibronectin on the honeycomb-patterned films.…”

Section: Morphologies Of Adherent Mature Hepatocytes and Small Hepatomentioning

confidence: 99%

Enhanced Cell Survival and Yield of Rat Small Hepatocytes by Honeycomb-Patterned Films

Tsukiyama

Matsushita

Tanaka

et al. 2008

jjap

Self Cite

View full text Add to dashboard Cite

Surface designing of substrate to regulate cell adhesion and function in nano and micro scale is a critical issue in biomaterial science. In this study, we describe the fabrication of highly regular patterned porous films (honeycomb-patterned film) formed by a simply casting technique, and the culture of mature hepatocytes and small hepatocytes on the films. The pore size of the honeycomb-patterned films used was 6, 12, and 16 mm. We evaluated the effect of the honeycomb-patterned films on the morphology, cell yield, survival and the differentiated hepatic function (albumin production) of the both hepatocytes. Both hepatocytes attached on the flat films appeared to spread well, showing a typical monolayer morphology. They peeled off from the films at 7 days in culture on the flat films. On the other hand, spreading of the each hepatocytes was restricted on the honeycomb-patterned films at 3 and 7 days in culture. The cell yield and survival of the each hepatocytes increased with increasing culture time. Small hepatocyte on the pore sizes of 16 mm showed the highest cell yield (approximately 3 times). Albumin production of mature hepatocyte on the pore sizes of 16 mm (224:1:3 AE 157 ng ml À1 well À1 at 1 day in culture, 369:5 AE 222 ng ml À1 well À1 at 3 days in culture) was higher than that of the hepatocytes on the flat films (119:3 AE 9:3 ng ml À1 well À1 at 1 day in culture, 262:8 AE 47:3 ng ml À1 well À1 at 3 days in culture), although that of small hepatocytes on the honeycomb-patterned films (pore size: 16 mm) was similar on the flat film. These results indicated that both the surface topography and the pore size of the honeycomb-patterned film affected the hepatic metabolic function.

show abstract

“…9 For example, the adhesion of cardiac myocytes and endothelial cells on honeycomb polycaprolactone (PCL) lms were regulated by the honeycomb pores via the inuence of pores on bronectin adsorption. 10,11 Additionally, the adhesion and proliferation of epidermal keratinocytes and dermal broblasts were promoted on honeycomb PCL with pore sizes ranging from 3 to 20 mm and the pore size from 3 to 5 mm showed the best cell response. 12 Fibroblasts also showed higher viability on honeycomb PCL lms (1 mm or 4 mm) than on at ones.…”

Section: Introductionmentioning

confidence: 99%