Relationship Between Activity of Gluthatione Peroxidase and Nitric Oxide in Synovial Fluid and the Progression of Temporomandibular Joint Internal Derangement

Abstract

The purposes of this study were to measure the activity of glutathione peroxidase (GPX) and nitric oxide (NO) in the synovial fluid of patients with temporomandibular joint (TMJ) internal derangement (ID) and to indicate the relationship between the activity of GPX and NO and the progress of the ID. Twenty-six patients with TMJ ID were identified and classified according to Wilkes staging through clinical and radiologic examinations. Levels of GPX were determined indirectly by a coupled reaction with glutathione reductase. Levels of NO were measured colorimetrically. The activity of GPX and NO was observed to be progressively increasing as the stage of the TMJ ID progressed. There were significant correlations between the 2 substances and the Wilkes stages. Oxidative stress may have a role in the pathogenesis of TMJ ID. In synovial fluid, GPX and NO activities are increased as the stage of the disease increased. Increase in the activities of GPX might not be enough to prevent progression of the TMJ ID.

Association between oxidative stress and temporomandibular joint dysfunction: A narrative review

“…As for the determination of the intra‐articular pathologic condition under study, studies were heterogeneous regarding the terminology used to classify the pathologic processes identified. The term internal derangement, described by Lin et al, Homma et al, Nagai et al, Takahashi et al, Tomida et al, Tomida et al, Guven et al and Guven et al, comprises a variety of intra‐articular changes, including disc displacement with reduction, described by Takahashi et al, Zardeneta et al, Suenaga et al, Cai et al, Etoz et al and Ishimaru et al and without reduction, described by Zardeneta et al, Suenaga et al and Cai et al Disc displacement with locking was another term used by the authors of the included studies . However, only Nitzan et al included the anchored disc phenomenon.…”

“…Increased production of free radicals derived from nitrogen was observed in four studies, which analysed the nitrite/nitrate concentration in synovial fluid and indirectly evaluated the presence of nitric oxide, because its short half‐life hinders a more reliable evaluation in the cellular environment. In addition, the presence of pain was associated with higher levels of nitrites/nitrates .…”

Research progress on CBCT of temporomandibular joint morphology in children with malocclusion in mixed dentition

“…CT is considered to be an accurate diagnostic imaging technique for evaluating temporomandibular joint, but CBCT is preferable due to its lower radiation dose and shorter scanning time [17] . Conical beam CT (CBCT) is widely used in the examination of oral diseases [18] , and also in the diagnosis and research of temporomandibular joint diseases [19] . Nakajima et al found that CBCT could provide three-dimensional images of the temporomandibular joint, in which the bone structure could be clearly observed [20] .…”

Inflammatory mediators related to arthrogenic temporomandibular dysfunctions

“…NO promotes muscle relaxation of blood vessels leading to vasodilation (a process that culminates in the formation of hyperemia and hyperthermia in inflammatory processes), besides reducing platelet aggregation 8 . In the TMJ, NO is involved in painful conditions 33 as well as in the pathogenesis and the progression of internal disorders 34 .…”