Relationship between aconitase gene expression and sporulation in Bacillus subtilis

Dingman, Douglas W.; Rosenkrantz, M S; Sonenshein, Abraham L.

doi:10.1128/jb.169.7.3068-3075.1987

Cited by 70 publications

(74 citation statements)

References 34 publications

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“…Spo0A does not control expression of all genes that are induced early during sporulation. For example, both citB Dingman et al 1987;Fouet and Sonenshein 1990) and ald (K. Jaacks Siranosian, K. Ireton, A.D. Grossman, in prep.) are required for sporulation and are expressed early during sporulation, independently of spoOA.…”

Section: Time (Hours)mentioning

confidence: 99%

Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor.

Ireton¹,

Rudner²,

Siranosian³

et al. 1993

Genes Dev.

247

221

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Multiple physiological and environmental signals are needed to initiate endospore formation in Bacillus subtilis. One key event controlling sporulation is activation of the Spo0A transcription factor. Spo0A is a member of a large family of conserved regulatory proteins whose activity is controlled by phosphorylation. We have isolated deletion mutations that remove part of the conserved amino terminus of Spo0A and make the transcription factor constitutively active, indicating that the amino terminus normally functions to keep the protein in an inactive state. Expression of an activated gene product is sufficient to activate expression of several sporulation genes in the absence of signals normally needed for initiation of sporulation. Our results indicate that nutritional, cell density, and cell-cycle signals are integrated through the phosphorylation pathway that controls activation of Spo0A.

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Section: Time (Hours)mentioning

confidence: 99%

Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor.

Ireton¹,

Rudner²,

Siranosian³

et al. 1993

Genes Dev.

247

221

View full text Add to dashboard Cite

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“…The cell growth rates and final pHs under the two sets of culture conditions did not differ significantly. ,B-Galactosidase activity was assayed as described by Dingman et al (6). An increase in f3-galactosidase synthesis in the presence of bicarbonate was observed for all three strains (Fig.…”

mentioning

confidence: 99%

The three Bacillus anthracis toxin genes are coordinately regulated by bicarbonate and temperature

1994

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The two BaciUlls anthracis toxins are composed of three proteins, protective antigen, lethal factor, and edema factor. The structural genes for these three components are located on the virulence plasmid pXOl. We constructed transcriptional fusions between the regulatory region of each of these genes and acZ. Each construct was then inserted as a single copy at the corresponding toxin gene locus on pXOl, resulting in three isogenic strains. Two environmental factors, bicarbonate and temperature, were found to induce 3-galactosidase synthesis in each recombinant strain. Furthermore, the transcription of the three toxin genes appears to be coordinately regulated.

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“…Some genes subject to such regulation, such as spoVG (12), are required for sporulation, but others, such as aprE (8), amyE (25), and gsiA (23), are not. The hut (histidine utilization) operon (1,26) and the citB gene (5,10), which encodes aconitase, are also induced early in stationary phase and are repressed independently by a mixture of amino acids and by rapidly metabolizable carbon sources. However, the molecular mechanisms of glucose repression and amino acid repression in B. subtilis remain elusive.…”

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confidence: 99%

Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon

1993

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The Bacillus subtilis dcU operon encodes a dipeptide transport complex that is induced rapidly as cells enter stationary phase and initiate sporulation. Expression of this operon in growing cells is repressed by glucose, by a mixture of amino acids, and by the AbrB protein. A genetic screen was devised to identify mutations that allow inappropriate expression from the dcL4 promoter during growth. These mutations resulted in increased dci4 transcription during growth in nutrient broth, in minimal amino acids medium, and in minimal glucose medium. Some of the mutations, called des (dci control site), were cloned and shown by sequence analysis to cluster near the start site of dci4 transcription. Primer extension and in vitro transcription analysis revealed that the dcs mutations did not create a new promoter. These mutations may therefore disrupt an operator site necessary for the binding of a negative regulator responsive to the nutritional state of the cell. The dcs mutant promoters were still subject to AbrB control, suggesting that the dci4 operon is regulated by at least two proteins, AbrB and a nutritionally responsive regulator. The gene(s) for the putative nutritional regulator may be defined by the cod (control ofdciA) mutations, which appeared to relieve amino acid and glucose repression ofdci4 by altering a diffusible factor. An abrB cod double mutant exhibited high-level expression ofdci4 during exponential growth phase.In response to conditions of carbon, nitrogen, or phosphorus limitation, Bacillus subtilis cells undergo a primitive metamorphosis to produce an environmentally resistant spore (19), but the mechanism by which initiation of sporulation is triggered by nutrient depletion is poorly understood (37). The expression of many genes induced as the cells enter stationary phase is known to be regulated by carbon and nitrogen sources (9) and to require the action of various signal transduction systems and transcriptional regulators (for reviews, see references 15 and 36). The relationship between these regulators and the nutritional response is obscure, however. Some genes subject to such regulation, such as spoVG (12), are required for sporulation, but others, such as aprE (8), amyE (25), and gsiA (23), are not. The hut (histidine utilization) operon (1, 26) and the citB gene (5, 10), which encodes aconitase, are also induced early in stationary phase and are repressed independently by a mixture of amino acids and by rapidly metabolizable carbon sources. However, the molecular mechanisms of glucose repression and amino acid repression in B. subtilis remain elusive. Our studies have focused on the regulation of the dci4 (decoyinine induced [21]) operon as a model system for understanding nutritional control phenomena that occur at the onset of Bacillus development.In previous work, we showed that the dci4 operon encodes a dipeptide transport system that is dispensable for sporulation but may assist the cells in adapting to poor nutrient conditions (20). This operon is transcribed at a very low lev...

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Relationship between aconitase gene expression and sporulation in Bacillus subtilis

Cited by 70 publications

References 34 publications

Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor.

Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor.

The three Bacillus anthracis toxin genes are coordinately regulated by bicarbonate and temperature

Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon

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