Relationship among circulating hemoglobin, nitric oxide synthase activities and angiogenic poise in red- and white-blooded Antarctic notothenioid fishes

Beers, Jody M.; Borley, Kimberly A.; Sidell, Bruce D.

doi:10.1016/j.cbpa.2010.03.027

Cited by 42 publications

(32 citation statements)

References 55 publications

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“…Owing to technical difficulties surrounding accurate direct measurement of NO, the aggregate concentration of NO x is often measured as a proxy for NO (Sun et al, 2003;Tsikas, 2005). Consistent with our hypothesis, we found that icefish species generally had higher concentrations of NO x in their plasma than did red-blooded species (Beers et al, 2010). When the results are adjusted for the larger blood volume of fish lacking Hb expression, it is clear that corporeal content of NO x is higher in icefishes than red-blooded notothenioids, strongly suggesting a corresponding difference in NO level.…”

Section: Introductionsupporting

confidence: 87%

“…Tissues of hemoglobinless icefishes display both dramatically greater mitochondrial densities (O'Brien and Sidell, 2000) and vascular densities (Wujcik et al, 2006) than those of their red-blooded relatives. Yet, despite the apparently higher level of NO in icefish, there is no significant difference in expression of mitochondrial biogenesis genes in ventricles and angiogenesis genes in retinae between red-and white-blooded adult notothenioids (Urschel and O'Brien, 2008;Beers et al, 2010). Feedback inhibition could be responsible for lack of upregulation in the genes, once stable well-oxygenated phenotypes are attained.…”

Section: Introductionmentioning

confidence: 99%

“…In previous work, we measured the concentration of nitrate plus nitrite (NO x ) in plasma of several species of notothenioids (Beers et al, 2010). Owing to technical difficulties surrounding accurate direct measurement of NO, the aggregate concentration of NO x is often measured as a proxy for NO (Sun et al, 2003;Tsikas, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Feedback inhibition could be responsible for lack of upregulation in the genes, once stable well-oxygenated phenotypes are attained. Indeed, NOS activity is lower in icefish than red-blooded species, indicating that feedback inhibition might account for a decrease in the rate of NO production in adult icefish (Beers et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Phenylhydrazine-induced anemia causes nitric-oxide-mediated upregulation of the angiogenic pathway in Notothenia coriiceps

Borley

Beers

Sidell

2010

Journal of Experimental Biology

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SUMMARYAntarctic icefishes possess several cardiovascular characteristics that enable them to deliver oxygen adequately in the absence of hemoglobin (Hb). To gain insight into mechanisms driving development of these cardiovascular characteristics of icefish, we chemically induced severe anemia in a red-blooded notothenioid, Notothenia coriiceps. After 10 days of treatment with phenylhydrazine HCl, the hematocrit and Hb concentration of N. coriiceps decreased by >90% and >70%, respectively. Anemic fish exhibited a significantly higher concentration of nitric oxide (NO) metabolites in their plasma compared with that of control animals, indicating that corporeal levels of NO are higher in anemic animals than in control fish. The activity of nitric oxide synthase (NOS) was measured in brain, retina, pectoral muscle and ventricle of control and anemic animals. With the exception of retina, no significant differences in NOS activities were observed, indicating that the increase in plasma NO metabolites is due to loss of Hb, which normally plays a major role in the degradation of NO, and not due to an overall increase in the capacity for NO production. To determine whether loss of Hb can stimulate remodeling of the cardiovascular system, we measured expression of HIF-1a, PHD2 and VEGF mRNA in retinae of control and anemic fish. Expression of all three genes was higher in anemic animals compared with control N. coriiceps, suggesting a causative relationship between loss of Hb and induction of angiogenesis that probably is mediated through nitric oxide signaling.

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Section: Introductionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenylhydrazine-induced anemia causes nitric-oxide-mediated upregulation of the angiogenic pathway in Notothenia coriiceps

Borley

Beers

Sidell

2010

Journal of Experimental Biology

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“…Icefishes serve as the natural knock-outs for functional studies of oxygen-binding hemoproteins and the correlated nitrogenmonoxide-oxygenase activity. Current research in mammals suggests that nearly all icefish hallmark traits are linked to high levels of NO (39).…”

Section: Discussionmentioning

confidence: 99%

Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations

Boron¹,

Russo²,

Boechi³

et al. 2011

IUBMB Life

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Neuroglobin (Ngb) is a heme protein, highly conserved along evolution, predominantly found in the nervous system. It is upregulated by hypoxia and ischemia and may have a neuroprotective role under hypoxic stress. Although many other roles have been proposed, the physiological function is still unclear. Antarctic icefishes lack hemoglobin and some species also lack myoglobin, but all have Ngb and thus may help the elucidation of Ngb function. We present the first theoretically derived structure of fish Ngb and describe its behavior using molecular dynamics simulations. Specifically, we sequenced and analyzed Ngbs from a colorless‐blooded Antarctic icefish species Chaenocephalus aceratus and a related red‐blooded species (Dissostichus mawsoni). Both fish Ngbs are 6‐coordinated but have some peculiarities that differentiate them from mammalian counterparts: they have extensions in the N and C termini that can interact with the EF loop, and a gap in the alignment that changes the CD‐region structure/dynamics that has been found to play a key role in human neuroglobin. Our results suggest that a single mutation between both fish Ngbs is responsible for significant difference in the behavior of the proteins. The functional role of these characteristics is discussed. © 2011 IUBMB IUBMB Life, 63(3): 206–213, 2011

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Evolutionary Adaptation and Disaptation in the Cold: the Icefish Paradigm

Tota

Amelio

Garofalo

et al. 2012

Adaptation and Evolution in Marine Environments, Volume 1

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Relationship among circulating hemoglobin, nitric oxide synthase activities and angiogenic poise in red- and white-blooded Antarctic notothenioid fishes

Cited by 42 publications

References 55 publications

Phenylhydrazine-induced anemia causes nitric-oxide-mediated upregulation of the angiogenic pathway in Notothenia coriiceps

Phenylhydrazine-induced anemia causes nitric-oxide-mediated upregulation of the angiogenic pathway in Notothenia coriiceps

Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations

Evolutionary Adaptation and Disaptation in the Cold: the Icefish Paradigm

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