Relations entre le taux de zinc dans le sang et l'activite de l'δ-aminolevulinic acid dehydratase erythrocytaire chez l'homme

Allain, Pierre; Foussard, V.E; Boyer, J.

doi:10.1016/0009-8981(77)90305-9

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…The activity of aminolaevulinic acid dehydratase is most frequently reduced by a heavy metal intoxication, commonly lead, but it is also decreased in various conditions: chronic ethylism (17,18), cirrhosis (19), smoking (20,21), renal insufficiency (22) and diabetes mellitus (23). All these causes were ruled out in our patient by anamnestic, clinical and biochemical investigations.…”

Section: Discussionmentioning

confidence: 81%

Biochemical Diagnosis of an Hereditary Aminolaevulinate Dehydratase Deflciency in a 63-Year-Old Man

Hassoun¹,

Verstraeten²,

Mercelis³

et al. 1989

Clinical Chemistry and Laboratory Medicine

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Summary:Porphyrin metabolism was investigated in a 63-year-old male patient who developed a subacute onset polyneuropathy with predominance of motor signs in the upper limb.The screening for lead, cadmium, mercury, aluminium and thallium was negative. The study of porphyrin metabolism showed remarkable abnormalities, particularly a very high level of plasmatic 5-aminolaevulinic acid contrasting with a normal level of porphobilinogen and a nearly complete loss of activity of aminolaevulinic acid dehydratase with no regenerative response to dithiothreitol or zinc ions. The other causes of aminolaevulinic acid dehydratase deficiency (tyrosinaemia, alcoholism, smoking, cirrhosis, renal insufficiency, diabetes mellitus) were ruled out.The diagnpsis of primary aminolaevulinic acid dehydratase deficiency was proposed and confirmed by the familial study, which revealed the existence of several heterozygous members in this family.

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Section: Discussionmentioning

confidence: 81%

Biochemical Diagnosis of an Hereditary Aminolaevulinate Dehydratase Deflciency in a 63-Year-Old Man

Hassoun¹,

Verstraeten²,

Mercelis³

et al. 1989

Clinical Chemistry and Laboratory Medicine

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“…Blood samples from 143 patients with blood lead levels ranging from 40 to 1,150 Mg/1 were collected in heparinized, polyethylene tubes free of lead and zinc (2). Blood lead was measured using a technique we have previously described (3).…”

Section: Methodsmentioning

confidence: 99%

“…All samples were stored at 4 °C in the dark, and assays were made within 4 h of sam pling, this lapse of time entailing practically no loss of enzyme activity (14,23). ALA-D activity was calcu lated from the molecular extinction coefficient of porphobilinogen, the hematocrit, the dilution and the incubation time (2). The final results were expressed in international units per liter of red blood cells.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Delta-Aminolevulinic Acid Dehydratase in Human Red Blood Cells by Lead and Activation by Zinc or Cysteine

Mauras¹,

Allain²

1979

Enzyme

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Inhibition of blood δ-aminolevulinic acid dehydratase(ALA-D) activity by lead was studied in vivo and in vitro. In vivo, a negative linear correlation (r = —0.85) was found between the logarithmic values of ALA-D activity and blood lead levels. In vitro the inhibitory effect of lead on blood ALA-D activity increased both with contact time and contact temperature of lead with blood before ALA-D assay. Maximum enzyme inhibition occurred after 14 h of contact at 25 °C. Inhibition of ALA-D activity by lead, in vivo as well as in vitro, is suppressed by the addition of zinc or cysteine. The logarithmic values of the activity ratios increase linearly with blood lead concentrations. The increase in ALA-D activity brought about by the addition of zinc or cysteine can be used to identify cases of low enzyme activity with no lead intoxication involved. The same technique can also detect cases in which ALA-D inhibition may be concealed by a presumably high initial enzyme activity as observed in some patients.

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“…Serum zinc is commonly determined by atomic absorption spectrophotometry (AAS) (3)(4)(5)(6)(7)(8)(9). Neutron activation analysis (NAA) is a very sensitive technique (10)(11)(12)(13)(14)(15) for the determination of this metal ion in biological fluids, but its use is restricted to laboratories that have access to a nuclear reactor.…”

mentioning

confidence: 99%

Determination of traces of zinc in biological materials, wine, and alloys by fluorometry

Jm¹,

Me²,

A³

1986

Anal. Chem.

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Relations entre le taux de zinc dans le sang et l'activite de l'δ-aminolevulinic acid dehydratase erythrocytaire chez l'homme

Cited by 6 publications

References 14 publications

Biochemical Diagnosis of an Hereditary Aminolaevulinate Dehydratase Deflciency in a 63-Year-Old Man

Biochemical Diagnosis of an Hereditary Aminolaevulinate Dehydratase Deflciency in a 63-Year-Old Man

Inhibition of Delta-Aminolevulinic Acid Dehydratase in Human Red Blood Cells by Lead and Activation by Zinc or Cysteine

Determination of traces of zinc in biological materials, wine, and alloys by fluorometry

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