Relations between nucleoli and nucleolus-organizing regions during the cell cycle

Goessens, Guy; Thiry, Marc; Lepoint, A

doi:10.1007/978-94-010-9166-4_25

Cited by 23 publications

(10 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further support for the crescent-shaped distribution of the Ag-NOR proteins we noted comes from the observation of a pair of silver spots in anaphase chromatids (Goodpasture and Bloom, 1975;Ploton et al, 1987b). However, the particu lar crescent-shaped distribution that we have found contrasts with the view of Goessens et al (1987). Analysis of serial sec tions of silver-stained mouse metaphase chromosomes led .…”

Section: Organization O F Ag-nor Proteinscontrasting

confidence: 66%

The Ag-NOR proteins present a crescent-shaped distribution at the secondary constrictions of metaphase PtK<sub>1</sub> chromosomes

Suja

Hernandez‐Verdun

1996

Cytogenet Genome Res

View full text Add to dashboard Cite

The ultrastructural distribution of the proteins responsible for the silver staining of NORs was analyzed on serial thin sections of preembedded silver-stained rat kangaroo PtKi metaphase chromosomes. Our results show that the Ag-NOR proteins present a crescent-shaped distribution at the secondary constriction of each chromatid. Moreover, in somecases the crescent-shaped structures of both chromatids are not symmetrically arranged but show different orientations. These observations, together with our own and previously reported light microscopy results obtained on silver-stained mammalian chromosomes, lead us to suggest a mechanism for the formation of apparent secondary constrictions at NORs.

show abstract

Section: Organization O F Ag-nor Proteinscontrasting

confidence: 66%

The Ag-NOR proteins present a crescent-shaped distribution at the secondary constrictions of metaphase PtK<sub>1</sub> chromosomes

Suja

Hernandez‐Verdun

1996

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…1b). Clear areas, the Wbrillar centers (FCs), are partly or entirely surrounded by a highly contrasted region (Goessens et al 1987), the dense Wbrillar component (DFC). The FCs and DFC are embedded in the granular component (GC), mainly composed of granules of 15-20 nm in diameter.…”

Section: General Features Of Nucleoli Observed By Microscopymentioning

confidence: 99%

The nucleolus: a model for the organization of nuclear functions

Hernandez‐Verdun

2006

Histochem Cell Biol

125

View full text Add to dashboard Cite

Nucleoli are the prominent contrasted structures of the cell nucleus. In the nucleolus, ribosomal RNAs (rRNAs) are synthesized, processed and assembled with ribosomal proteins. The size and organization of the nucleolus are directly related to ribosome production. The organization of the nucleolus reveals the functional compartmentation of the nucleolar machineries that depends on nucleolar activity. When this activity is blocked, disrupted or impossible, the nucleolar proteins have the capacity to interact independently of the processing activity. In addition, nucleoli are dynamic structures in which nucleolar proteins rapidly associate and dissociate with nucleolar components in continuous exchanges with the nucleoplasm. At the time of nucleolar assembly, the processing machineries are recruited in a regulated manner in time and space, controlled by different kinases and form intermediate structures, the prenucleolar bodies. The participation of stable pre-rRNAs in nucleolar assembly was demonstrated after mitosis and during development but this is an intriguing observation since the role of these pre-rRNAs is presently unknown. A brief report on the nucleolus and diseases is proposed as well as of nucleolar functions different from ribosome biogenesis.

show abstract

“…Following the arrest of the transcription processes [27], the chromosomes condense, the nuclear envelope breaks down and the nuclear constituents are disassembled. It is noteworthy that nucleoli disappear at the beginning of prophase and that their proteins are redistributed at specific locations in mitotic cells (see for reviews [8,11,14,34,38]): i) some proteins remain associated with the secondary constriction in which rDNA clustered [30,31,35]; ii) other nucleolar proteins are soluble in the cytoplasm [36]; iii) the third class of nucleolar proteins lines the chromosome periphery during mitosis. These nucleolar proteins disperse at the periphery of all chromosomes forming a novel class of chromosomal passenger proteins, the nucleolar perichromosomal (NoPC) proteins [12].…”

Section: Introductionmentioning

confidence: 99%

Fate of specific nucleolar perichromosomal proteins during mitosis: Cellular distribution and association with U3 snoRNA

Gautier

Fomproix

Masson

et al. 1994

Biology of the Cell

View full text Add to dashboard Cite

In mammalian cells, the nucleoli disintegrate during mitosis and some nucleolar proteins disperse at the periphery of all chromosomes forming a novel class of chromosomal passenger proteins. The nucleolar components which participate in the formation of this perichromosomal layer have been investigated to elucidate the role of these perichromosomal proteins in the assembly and disassembly of the nucleoli. i) Electron microscopy immunolabelling reveals that these proteins are predominantly located in the granular component of the nucleoli during interphase. ii) Immunoprecipitation data suggest that they are distributed at the chromosome periphery in association with U3 small nucleolar RNA (snoRNA). In addition, the distribution of U3 snoRNA visualized by in situ hybridization, is similar to that observed for the perichromosomal proteins. iii) In cells which possess a nucleolar remnant during mitosis, U3 snoRNA and perichromosomal proteins were found both in the perichromosomal layer and in the nucleolar remnant. iv) Some of these proteins are conserved from yeast to man such as fibrillarin and a protein of 52 kDa. v) The location of these proteins observed in yeast by confocal microscopy shows that they are not dispersed during mitosis. Their partition between the two daughter cells is performed by scission of nucleolar structures forming a rod during the budding process. Therefore RNP complexes related to the processing steps of ribosome biogenesis in mammalian cells quit the nucleolus in late G2 and associate with the chromosome periphery until late telophase. They associate in the perichromosomal layer in human and PtK1 cells and both in the perichromosomal layer and the nucleolar remnant in CHO cells.

show abstract

Relations between nucleoli and nucleolus-organizing regions during the cell cycle

Cited by 23 publications

References 34 publications

The Ag-NOR proteins present a crescent-shaped distribution at the secondary constrictions of metaphase PtK<sub>1</sub> chromosomes

The Ag-NOR proteins present a crescent-shaped distribution at the secondary constrictions of metaphase PtK<sub>1</sub> chromosomes

The nucleolus: a model for the organization of nuclear functions

Fate of specific nucleolar perichromosomal proteins during mitosis: Cellular distribution and association with U3 snoRNA

Contact Info

Product

Resources

About