Relations among Steady State, Time Domain, and Frequency Domain Fluorescence Quenching Rates. 2

Abstract

A nonequilibrium statistical thermodynamic approach (Molski, A.; Keizer, J. J. Phys. Chem. 1993, 97, 8707) developed to study relations among rate coefficients for steady state, time domain, and frequency domain fluorescence quenching is extended to the case where static quenching is present. An exact, model-independent expression is derived for the mean-field, frequency-dependent rate coefficient k"f in the linear harmonic regime.This expression is a rigorous formulation of the relationship whose approximate form was derived in our previous work. It is argued that the relations between various quenching rate coefficients are analogous with those valid in the absence of static quenching. Treatments of fluorescence quenching kinetics by Sung et al. and by Zhou and, Szabo are briefly commented upon.