Relation of phospholipase D activity to the decay of succinate dehydrogenase and of covalently bound flavin in yeast cells undergoing glucose repression

Phospholipase D: Enzymology, Functionality, and Chemical Modulation

“…Yeast harvested from these growth conditions demonstrated 14 C-lecithin hydrolysis and PA production in mitochondrial fractions. 265 This activity was increased in response to glucose repression during aerobic growth and decreased oxygen uptake. The increased activity was determined to be due to induction of an unknown cytosolic enzyme rather than a protein of mitochondrial origin since cyclohexamide blockage of cytosolic protein synthesis perturbed the PLD activity, and chloramphenicol inhibition of mitochondrial protein synthesis did not.…”

“…The increased activity was determined to be due to induction of an unknown cytosolic enzyme rather than a protein of mitochondrial origin since cyclohexamide blockage of cytosolic protein synthesis perturbed the PLD activity, and chloramphenicol inhibition of mitochondrial protein synthesis did not. 265 This observation was largely ignored until a series of parallel studies decades later identified specific PLD enzymes in different yeast species. Spo14, a PLD superfamily member also known as PLD1, was identified in the budding yeast Saccharomyces cerevisiae.…”

“…In the 1970s, Dharmalingam et al 264 and Grossman et al 265 described glucose-stimulated PLD activity in S. cerevisiae . More recent characterization of yeast PLD activities suggests this observed PLD activity is due to a separate class of enzyme, likely that of PLD2.…”

Identification of a novel, Ca2+‐dependent phospholipase D with preference for phosphatidylserine and phosphatidylethanolamine in Saccharomyces cerevisiae

“…PLD in the yeast Saccharomyces cerevisiae was suggested to be involved in the disintegration of mitochondria in cells undergoing glucose repression [6,7]. Recently a PLD that is induced during sporulation was identified in yeast [8][9][10][11].…”

Characterisitics and Gene Cloning of Phospholipase D of the Psychrophile,Shewanellasp.

“…1) The activity of PLD has been identified in various organisms, including mammals, 2) plants, 3) yeast, 4) and bacteria. 5,6) With bacterial PLD, it was found that Streptomyces hachijoensis and Streptomyces chromofuscus produce a secretion type of enzyme with maximal hydrolytic activity around pH 8.0, and that Ca 2þ ion is essential for their activities.…”