Relation of lysozyme concentration to acquired immunity against Pseudomonas aeruginosa in Galleria mellonella

Chadwick, June S.

doi:10.1016/0022-2011(70)90191-6

Cited by 56 publications

(15 citation statements)

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“…However, due to the higher molecular weight of HEWL (14 kDa), their inhibition efficiency by weight is in the same range. This low K i and ID 50 are compatible with the physiological environment of insects [20–22], which suggests that lysozyme may be involved in the physiological control of melanin production in these organisms.…”

Section: Discussionmentioning

confidence: 73%

Hen egg white lysozyme as an inhibitor of mushroom tyrosinase

Huang

Paskewitz

2006

FEBS Letters

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We report a kinetics study on hen egg white lysozyme's (HEWL) inhibitory effect on mushroom tyrosinase catalysis of 3-For the first time, we demonstrate HEWL as a robust inhibitor against mushroom tyrosinase in catalysis of both substrates. The kinetics pattern matches a mixed (mostly non-competitive) partial inhibition. K i and ID 50 value of HEWL are more than 20-fold lower than that of kojic acid, a well-known chemical inhibitor of mushroom tyrosinase. K i , a value and b value, are almost identical in both experiments (L L-DOPA and L L-tyrosine as substrates, respectively), which suggests this common inhibition mechanism affects both steps. The inhibitory effect increases as both proteins were mixed and pre-incubated for less than 1 h. HEWL-depletion only removed about half of the inhibitory effect. Here we propose a novel function of HEWL, which combines the reversible inhibition and the irreversible inactivation toward mushroom tyrosinase. Discovery of HEWL as an inhibitor to mushroom tyrosinase catalysis may be commercially valuable in the food, medical and cosmetic industries.

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Section: Discussionmentioning

confidence: 73%

Hen egg white lysozyme as an inhibitor of mushroom tyrosinase

Huang

Paskewitz

2006

FEBS Letters

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“…Lysozyme may have a substantial role enabling G. mellonella to contain Gram-positive bacteria (Mohrig & Messner, 1968;IVIohrig, Storz & Messner, 1970;Mohrig, Schittek & Hanschke, 1979). Chadwick (1970) reported that lysozyme did not confer protection to immune G. mellonella against P. aeruginosa. Dunphy, proposed that lysozyme in non-immune G. mellonella assisted in the destruction of rough-walled mutants of P. aeruginosa.…”

Section: Discussionmentioning

confidence: 99%

Influence ofSteinernema feltiae(Filipjev) Wouts, Mracek, Gerdin and Bedding DD136 strain on the humoral and haemocytic responses ofGalleria mellonella(L.) larvae to selected bacteria

Dunphy

Webster

1985

Parasitology

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Examination of the short-term interaction of the haemocytes and lysozyme of Galleria mellonella larvae with the entomogenous nematode Steinernema feltiae DD136, in vitro revealed that the nematodes did not reduce the adhesion of Bacillus subtilis or Xenorhabdus nematophilus subsp. nematophilus to larval granulocytes or plasmatocytes. There was no evidence of humoral, sheath or cellular encapsulation of S. feltiae in the haemolymph in vitro or in vivo. Compared with the phosphate-buffered saline-injected larvae the axenic nematodes did not alter the total or differential haemocyte counts during the initial 4 h of parasitism. The ability of the insect larvae to remove B. subtilis and X. nematophilus from the haemolymph was not influenced by axenic S. feltiae. The bacteria from the intestine of surface disinfected, monoxenically cultured S. feltiae elevated the larval total haemocyte counts and damaged the haemocytes. The activity of larval lysozyme was not influenced by axenic S. feltiae.* Decoded mean (with 9 5 % confidence limits of 2 arcsin-transformed data), n = 25 larvae, 100 haemocytes/larva examined. t Sp, spherulocyte; PI, plasmatocyte; Gr, granulocyte; Oe, oenocytoid. % Phosphate-buffered saline.

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“…The enzymes from Bombyx and Ga lleria have been purified (Table 2) and some kinetic differ ences from egg white lysozyme, as well as a higher specific activity, have been fo und (178,191). After injection of killed bacteria, hemolymph lyso zyme activity may rise greatly (178,449,450), and a primary role of lysozyme in antibacterial defense has been suggested (450). Quantitatively, however, the lysozyme activity does not parallel the bacterial resistance (442,449) and the hemolymph lysozyme has bee n separated from other bactericidal fa ctors (437,442,444 ), so that it appears to be only one compo nent of the antibacterial system.…”

Section: Inducible Antibacterial Fa Ctorsmentioning

confidence: 99%