Relation of Dipicolinic Acid Content of Anaerobic Bacterial Endospores to Their Heat Resistance

Byrne, Anne F.; Burton, Thomas H.; Koch, Rainer

doi:10.1128/jb.80.1.139-140.1960

Cited by 15 publications

(6 citation statements)

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“…An inspection of the values for DPA content (Tables 2 and 3) shows little quantitative relationship between this compound and heat resistance. Similar findings were reported by Byrne, Burton, and Koch (1960) in some preliminary experiments on the heat survival of spores of Clostridium roseum. Although the presence of DPA is essential for heat-resistant spores (Church and Halvorson, 1959;Hashimoto et al, 1960), the accumulation of this compound beyond a given level seems to have little influence on thermal stability.…”

Section: And Discussionsupporting

confidence: 90%

Chemical Composition and Heat Resistance of Some Aerobic Bacterial Spores

1961

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WALKER, HOMER W. (Iowa State University of Science and Technology, Ames), JACK R. MATCHES, AND JOHN C. AYRES. Chemical composition and heat resistance of some aerobic bacterial spores. J. Bacteriol. 82:960-966. 1961.-Analyses of spores of Bacillus species for nitrogen, carbohydrate, dipicolinic acid, and phosphorus showed little correlation with heat resistance. However, as the molar concentration of magnesium increased in relation to dipicolinic acid and calcium concentrations, heat resistance generally decreased. Analyses of several batches of spores indicated that this relationship between calcium, magnesium, and dipicolinic acid did not always hold true. Therefore, while these materials apparently play an important role, other factors need to be included before a full explanation of thermal stability of spores can be made.

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Section: And Discussionsupporting

confidence: 90%

Chemical Composition and Heat Resistance of Some Aerobic Bacterial Spores

1961

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“…In addition to the accumulation of metal ions, spores also contain a relatively large amount of 2,6-pyridinedicarboxylic acid (dipicolinic acid, DPA) (21,20) as well as a specific spore peptidoglycan (21,18). DPA is present in amounts of 5-15 % (on dry weight basis) (5,3) and is distinguished by its ability to strongly chelate many divalent metals (27,31,1).…”

Section: Introductionmentioning

confidence: 99%

Coordinative Binding of Divalent Cations with Ligands Related to Bacterial Spores

Chung¹,

Rajan²,

Merdinger³

et al. 1971

Biophysical Journal

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It has been repeatedly postulated that the high heat resistance of bacterial spores is due to stabilization of biopolymers in the spore interior by a solid deposit of protective cement consisting of coordination complexes of ligands with divalent metal ions. This report presents data on metal-binding characteristics of some of the ligands related to spores as determined by means of potentiometric equilibrium measurements under conditions of temperature and ionic strength (t = 25.0 degrees C; mu = 1.0 KNO(3)) identical with those reported earlier by the authors in order to facilitate correlation by using comparable data. The spore ligands investigated in this study included 2,6-pyridinedicarboxylic acid (DPA), alpha,epsilon-diaminopimelic acid, D-glutamic acid, and D-alanine in a ratio of 1:1 with metal ions which are known to play a role in heat resistance of spores. Stability constants of the chelates of these spore ligands with metal ions such as Ca(II), Mg(II), Cu(II), Ni(II), Zn(II), Co(II), and Mn(II) have been determined. In general the metal chelates of DPA exhibited the greatest stability. On the basis of a consideration of the stability data together with the known configurations of the ligand and the coordination requirements of the metal ions, possible structures indicating the coordinate binding of the spore ligands with the metal ions are presented. All the metal chelates except those of Ca(II) were found to undergo hydrolysis and separation of solid phase in the pH range 7-8.5. The relatively greater hydrolytic stability of Ca(II) chelates and the high affinity of DPA for metal ions appear to be of biological significance insofar as these two spore components are more widely associated with the heat resistance of bacterial spores.

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“…High concentration of DPA was related to low heat resistance (Byrne et al, 1960). Reports suggested that higher molar ratios Ca:DPA resulted in higher heat tolerance (Levinson et al, 1961) and that calcium was essential to attain maximum thermo-resistance (Foerster et al, 1966).…”

Section: Methodsmentioning

confidence: 99%