Relation of Cell Wall Lipid Content of
            <i>Serratia marcescens</i>
            to Resistance to Antimicrobial Agents

Winshell, Elaine B.; Neu, Harold C.

doi:10.1128/aac.6.1.73

Cited by 12 publications

(5 citation statements)

References 22 publications

(7 reference statements)

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“…However, our results of this and other studies (15) do not seem to strongly support the implication that pigmented cells in general are more susceptible to antibiotics (5,22). The study of Winshell and Neu (22) seemed to indicate that clinical isolates of pigmented strains of different bacteriocin types were generally more susceptible to diverse antibiotics than were nonpigmented strains.…”

contrasting

confidence: 54%

Antibiogram and Lipid Analysis of a Pigmented Strain of Serratia marcescens and Its Nonpigmented Variants

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Miller

Tsang

1975

Antimicrob Agents Chemother

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confidence: 54%

Antibiogram and Lipid Analysis of a Pigmented Strain of Serratia marcescens and Its Nonpigmented Variants

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Miller

Tsang

1975

Antimicrob Agents Chemother

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“…The greatest impediment to the successful treatment of bacterial diseases is antibiotic resistance that is widespread and occurs in numerous bacterial genera [2]. Winshell and Neu [3] divided Serratia marcescens strains into three groups on the basis of antimicrobial resistance and pigment production. Group I, comprising non-pigmented strains, are resistant to most antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Heavy-Metal Tolerance and Antibiotic Susceptibility of Red Pigmented Bacteria Isolated from Marine Environment

Jafarzade¹,

Mohamad²,

Usup³

et al. 2012

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This study was undertaken to determine heavy metal resistance and antibiotic susceptibility of three non-pathogenic red pigmented bacteria namely WPRA3, SM11-3j and SC-G18, isolated from marine environments of Malaysia. The bacteria isolates were identified by 16S rRNA sequencing and by biochemical and morphological tests. The 16S rRNA gene sequences of all isolates showed ≥96% similarity to Serratia spp. Antibiotic susceptibility test of isolates was assayed according to the Kirby-Bauer disc diffusion method. All isolates were highly resistant to beta-lactam antibiotics, but were susceptible to quinolone antibiotics. . Isolates WPRA3 and SM11-3j showed higher multiple tolerances to heavy metals. The results obtained indicate that bacteria from marine environments of Malaysia present interesting metabolic activities, which should be studied and explored for potential biotechnological applications.

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“…The major identifier that differentiates S. marcescens from other enteric bacteria is the cell associated prodigiosin production (Mahlen, 2011). But, many species of the genus Serratia, are non-pigmented or vary widely in pigmentation (Winshell and Neu, 1974).…”

Section: Isolation and Identification Of The Serratia Marceacensmentioning

confidence: 99%

Effect of Prodigiosin on Biofilm Formation in Clinical Isolates of Pseudomonas Aeruginosa

Dhaife¹,

Al-Attar²

2021

PLANT ARCHIVES

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From the duration between August to December 2019, a total of 120 burn and wound specimens were collected from Baghdad hospitals. The obtained specimens were streaked directly on macConkey and cetramide agar plates, followed by further biochemical testing; 46 isolates were identified as Pseudomonas aeruginosa. Furthermore, the identification was confirmed using Vitek-2 compact system. Moreover; from the 120 obtained specimens, four isolates were identified as Serratia marcescens (prodigiosin producers) using biochemical tests and Vitek-2 compact system. Mineral salt broth with pepton (0.5%) was used, followed by prodigiosin pigment extraction, purification and determination the concentration of the prodigiosin pigment, During prigment extraction, the four isolates of S. marcescens (S1, S2, S3, S4) were cultured singly and also cultured by mixing them randomly. The results showed that mixing Serratia isolates led to further results, the production of pigment was better by mixing two isolates (S1+S2). Antibiotic susceptibility testing of all (46) P. aeruginosa isolates to Ciprofloxacin, Amikacin, Tobramycin, Cefotaxime, Azteronam and Meropenem was assessed using Kirby-Bauer disk diffusion assay. The result revealed that 46 (100%) isolates were resistant to Amikacin; additionally, 50%, 48%, 43% and 28% isolates were resistant to Tobramycin, Cefotaxime, Ciprofloxacin and Azteronam respectively. On the other hand, Meropenem revealed to be most effective drug since it recorded the highest sensitivity percentage of 98%. Moreover, the bacterial ability for the formation of biofilm was assessed for 30 selected P. aeruginosa isolates (multi drug resistant isolates) using microtiter plate assay, the result indicated that only 5 strong isolates were biofilm producer whereas 18 and 7 isolates were moderate and weak biofilm producers respectively. DNA of P. aeruginosa were successfully extracted from overnight cultures, and PCR was conducted to amplify constitutional genes 16srRNA, AlgD, the bands were confirmed with gel electrophoresis, the results showed that genes 16srRNA (956 bp) and AlgD (313bp) were detected. The presence of AlgD gene was tested in 17 selected P. aeruginosa isolates (strong and moderate biofilm producers) using polymerase chain reaction technique; however, only 2 isolates (P9 and P21) harbored AlgD gene. The minimal inhibitory concentrations of prodigiosin and Ciprofloxacin were assessed for P. aeruginosa isolates (P9 and P21) using broth microdilution method; even more, the synergistic effect of prodigiosin combined with the Ciprofloxacin was also assessed for the same tested isolates. The result revealed that Ciprofloxacin minimum inhibitory concentration values were 31.2 µg/ml and 15.6 µg/ml for P. aeruginosa(P9 and P21) isolates respectively, Additionally, the minimum inhibitory concentration value for prodigiosin pigment were 87.5 µg/ml and 175 µg/ml for P. aeruginosa (P9 and P21) isolates respectively; regarding the synergistic effect the Ciprofloxacin combined with the prodigiosin, the result reve...

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Relation of Cell Wall Lipid Content of Serratia marcescens to Resistance to Antimicrobial Agents

Cited by 12 publications

References 22 publications

Antibiogram and Lipid Analysis of a Pigmented Strain of Serratia marcescens and Its Nonpigmented Variants

Antibiogram and Lipid Analysis of a Pigmented Strain of Serratia marcescens and Its Nonpigmented Variants

Heavy-Metal Tolerance and Antibiotic Susceptibility of Red Pigmented Bacteria Isolated from Marine Environment

Effect of Prodigiosin on Biofilm Formation in Clinical Isolates of Pseudomonas Aeruginosa

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