Relation between Silver Nanoparticle Formation Rate and Antioxidant Capacity of Aqueous Plant Leaf Extracts

Abstract

Correlation between the antioxidant capacity and silver nanoparticle formation rates of pomegranate (Punica granatum), quince (Cydonia oblonga), chestnut (Castanea sativa), fig (Ficus carica), walnut (Juglans cinerea), black mulberry (Morus nigra), and white mulberry (Morus alba) leaf extracts is investigated at a fixed illumination. Silver nanoparticles formed in all plant leaf extracts possess round shapes with average particle size of 15 to 25 nm, whereas corresponding surface plasmon resonance peak wavelengths vary between 422 nm and 451 nm. Cupric reducing antioxidant capacity technique is used as a reference method to determine total antioxidant capacity of the plant leaf extracts. Integrated absorbance over the plasmon resonance peaks exhibits better linear relation with antioxidant capacities of various plant leaf extracts compared to peak absorbance values, with correlation coefficient values of 0.9333 and 0.7221, respectively.

Measurement of antioxidants in distilled spirits by a silver nanoparticle assay

“…Future work will include the analysis of lyoniresinol, the main polyphenolic compound in whisky (6). Akbal et al (18) determined that the area under the curve of the SPR band by UV-Vis analysis resulted in better correlation against the Trolox equivalence (an indicator of TAC) in plant extracts of that study and this too is an area of future examination. Understanding the origin of many these antioxidants, whether they are extracted from barrels used in aging or from the grain used, would also be of interest.…”

“…Recent interest in utilizing metal nanoparticle (NP) assays for determination of TAC in food and plant extract samples has been growing . Many of these NP assays have been validated against other methods, including the Folin–Ciocalteu assay or CUPRAC assay and one gold nanoparticle assay has been validated with quantitative HPLC measurements . Özyürek et al .…”

“…Recent interest in utilizing metal nanoparticle (NP) assays for determination of TAC in food and plant extract samples has been growing (16)(17)(18)(19)(20)(21). Many of these NP assays have been validated against other methods, including the Folin-Ciocalteu assay (19) or CUPRAC assay (17,18) and one gold nanoparticle assay has been validated with quantitative HPLC measurements (21). Özyürek et al (17) provided an excellent report of a developed and validated silver nanoparticle antioxidant capacity (SNPAC) assay for the measurement of the antioxidant capacity of polyphenols.…”

Biosynthesis, characterisation and therapeutic applications of plant-mediated silver nanoparticles

“…The majority of plants used for the synthesis of AgNPs belong to the following families: Acanthaceae, Amaranthaceae, Apocynaceae, Asteraceae, Burseraceae, Dioscoreaceae, Euphorbiaceae, Fabaceae, Lamiaceae, Moraceae, Myrtaceae, Poaceae, Ranunculaceae, Rutaceae, Solanaceae and Asphodelaceae. 1 Among the plants used for the synthesis of AgNPs, the most frequent were: Punica granatum, Cydonia oblonga, Castanea sativa, Ficus carica, Juglans cinerea, Morus nigra, Morus alba, 12 Piper nigrum, 13,14 Morinda citrifolia, 15 Calendula officinalis, 16 Glycyrrhiza glabra, 17 Artemisia annua, 18 Sida acuta, 18 Emblica officinalis, Terminalia catappa, Eucalyptus hybrida, 19 Choerospondias axillaris, Nyctanthes arbortristis, Moringa oleifera, Zanthoxylum armatum, Areca catechu, 20 Argemone Mexicana and Ocimum tenuiflorum. 21 Biomolecules that participate in reduction and capping of the nanoparticles are distributed in the leaves, stems, fruit, seeds and roots, and belong to the following classes of compounds: alcoholic compounds, alkaloids, amino acids, phenols, flavones, enzymes, polysaccharides, proteins, terpenoids, some vitamins, chlorophyll and other metabolites.…”

“…37 Different antioxidants with varied structures present in Ananas comosus juice act synergistically in reducing silver ions. 38 For the extraction of biomolecules, solvents such as water, 12,16 ethanol, 39,40 70 % ethanol, 41 etc. are used.…”