Relation between Ovarian Carcinoma-Associated Antigens in Tumor Tissue and Detached Cyst Fluid Cells of Patients with Ovarian Neoplasms

Jk, Bar; Harłozińska, A; Sobańska, Ewa; Cisło, M

doi:10.1177/030089169408000110

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…As shown in Figure 1, a highly significant difference was observed between the cat B in tumour samples before and after treatment with placental CPI (p< 0.0001). Immunohistochemical staining was performed according to Bar et al (1994) using a 3-stage peroxidaseantiperoxidase technique. To determine relationships between different sources, double immunodiffusion was carried out with the antibodies directed against placental CPI.…”

mentioning

confidence: 51%

Expression of High Molecular Weight Cysteine Proteinase Inhibitor in Ovarian Cancer Tissues: Regulation of Cathepsin B Expression by Placental CPI

Siewiński

Saleh²,

Popiela³

et al. 2003

Biological Chemistry

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Mouse polyclonal antibodies against placental cysteine proteinase inhibitor (CPI) react with the placental 67 kDa CPI on Western blots, and CPI present in ovarian cancer homogenate and serum was shown by double immunodiffusion to react with the same antiserum. By immunohistochemical staining, positive expression of high molecular weight CPI was observed on the tumour cell surface in serous and endometrioid ovarian carcinomas with metastasis. Normal endometrioid tissue was not stained with anti-placental CPI antibodies. Cathepsin B and pro-cathepsin B median levels in ovarian cancer tissue homogenates increased progressively with FIGO stage of the disease. The enzyme level decreased 22-fold after treatment of tissue homogenates with 5 nM purified CPI. These results provide evidence that addition of CPI reduces the levels of cysteine-type cathepsins to those of normal non-cancerous values.

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mentioning

confidence: 51%

Expression of High Molecular Weight Cysteine Proteinase Inhibitor in Ovarian Cancer Tissues: Regulation of Cathepsin B Expression by Placental CPI

Siewiński

Saleh²,

Popiela³

et al. 2003

Biological Chemistry

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“…Ovarian tumours typically present as large cystic masses, consisting of variable amounts of fluid which has proven to contain many compounds that reflect the biological processes taking place within the ovarian epithelial tumour wall [14]. Clinically, oCF cannot be used for differentiation between benign and malignant tumours as pre-operative punction of the ovarian tumour might cause metastatic tumour spread.…”

Section: Original Articlementioning

confidence: 99%

“…Recently, we explored levels of cathepsin B, L and their inhibitor CysC in cyst fluid of epithelial ovarian tumours and found significantly higher levels for patients with EOC compared with patients with benign lesions [13]. Ovarian tumours typically present as large cystic masses, consisting of variable amounts of fluid which has proven to contain many compounds that reflect the biological processes taking place within the ovarian epithelial tumour wall [14]. Clinically, oCF cannot be used for differentiation between benign and malignant tumours as pre-operative punction of the ovarian tumour might cause metastatic tumour spread.…”

Section: Original Articlementioning

confidence: 99%

The balance between extracellular cathepsins and cystatin C is of importance for ovarian cancer

Kolwijck

Kos

Obermajer

et al. 2010

Eur J Clin Investigation

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“…The cyst fluid has been shown to contain compounds released by the tumor tissue (4,5), and therefore, might provide biomarkers for ovarian cancer. Although researchers have become increasingly interested in the analysis of the biochemical composition of cyst fluid, only a few studies have been performed investigating cyst fluid using MRS (6)(7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

N‐acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors

et al. 2009

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An unassigned and prominent resonance in the region from delta 2.0-2.1 ppm has frequently been found in the in vivo MR spectra of cancer patients. We demonstrated the presence of this resonance with in vivo MRS in the cyst fluid of a patient with an ovarian tumor. (1)H-NMRS on the aspirated cyst fluid of this patient confirmed the observation. A complex of resonances was observed between 2.0 and 2.1 ppm. It was also present in 11 additional ovarian cyst fluid samples randomly chosen from our biobank. The resonance complex was significantly more prominent in samples from mucinous tumors than in samples from other histological subtypes. A macromolecule (>10 kDa) was found responsible for this complex of resonances. A correlation spectroscopy (COSY) experiment revealed cross peaks of two different types of bound sialic acid suggesting that N-glycans from glycoproteins and/or glycolipids cause this resonance complex. In the literature, plasma alpha-1 acid glycoprotein (AGP), known for its high content of N-linked glycans, has been suggested to contribute to the delta 2.0-2.1 spectral region. The AGP cyst fluid concentration did not correlate significantly with the peak height of the delta 2.0-2.1 resonance complex in our study. AGP may be partly responsible for the resonance complex but other N-acetylated glycoproteins and/or glycolipids also contribute. After deproteinization of the cyst fluid, N-acetyl-L-aspartic acid (NAA) was found to contribute significantly to the signal in this spectral region in three of the 12 samples. GC-MS independently confirmed the presence of NAA in high concentration in the three samples, which all derived from benign serous tumors. We conclude that both NAA and N-acetyl groups from glycoproteins and/or glycolipids may contribute to the delta 2.0-2.1 ppm resonance complex in ovarian cyst fluid. This spectral region seems to contain resonances from biomarkers that provide relevant clinical information on the type of ovarian tumor.

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Relation between Ovarian Carcinoma-Associated Antigens in Tumor Tissue and Detached Cyst Fluid Cells of Patients with Ovarian Neoplasms

Cited by 7 publications

References 14 publications

Expression of High Molecular Weight Cysteine Proteinase Inhibitor in Ovarian Cancer Tissues: Regulation of Cathepsin B Expression by Placental CPI

Expression of High Molecular Weight Cysteine Proteinase Inhibitor in Ovarian Cancer Tissues: Regulation of Cathepsin B Expression by Placental CPI

The balance between extracellular cathepsins and cystatin C is of importance for ovarian cancer

N‐acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors

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