Relation between Macroscopic Binding Constant and the Anticancer Efficacy of the Bovine Serum Albumin-Quercetin Complex against Drug-Sensitive and Drug-Resistant Cells

Abstract

Problem statement:We have previously analyzed the interaction of BSA with flavonoids by using FRET. In this study, the role of BSA on increasing in solubility and on carrying the quercetin derivatives thus enhanced their anticancer efficacy against drug-sensitive and drug-resistant cells were conducted. ) at C3 yielded an increase in stability of the complexes. Rutin was tightly bound to BSA resulting in the changes in mode of action. Conclusion: The macroscopic binding constant was directly influenced on the cellular uptake of molecules and the suitable range of binding constant was K D ≥10 5 M −1 by which the carrier can be useful for increasing the solubility of drug and also spontaneously release the drug into the solution and cells.

Quercetin Influence on Thermal Denaturation of Bovine Serum Albumin

“…A specific insight into the energetics of QUER–protein interactions seems to be missing. The binding parameters obtained from spectroscopic methods reported for QUER–serum albumin binding were found to vary from 7.60 × 10 4 M –1 to 8.38 × 10 6 M –1 for the binding constant ( K ) and from a single class of binding site within site I of BSA and HSA − to two different sites of HSA, − both situated in the vicinity of the single tryptophan moiety at position 214. The broad range of binding parameters obtained in the above studies indicates the importance of the experimental approach and the limitations inherent to each method.…”

“…The interaction of serum proteins with QUER and/or other flavonol derivatives has been previously investigated. − Most of these studies are focused on either monitoring of the ligand-protein interaction using the ligand-induced fluorescence quenching of the protein, or the binding mechanism analysis in terms of only conformational aspects using fluorescence, circular dichroism, FTIR spectroscopy, electronic absorption spectroscopy, and NMR spectroscopy.…”

EXTRACTION OF QUERCETIN FROM <i>POLYGONUM HYDROPIPER</i> L. BY SUBCRITICAL WATER

“…Flavones and flavonoid glycosides, such as quercetin galactosides, a sesquiterpene acid, viscosumic acid, oxymethylanthraquinones and polygonic acid were identified in all parts of the plant (Furukawa et al, 2002). The plant also has some insecticidal (Kundu et al, 2007), bitter, stimulant, tonic, diuretic, carminative, anthelmintic, haemostatic and lithotripter properties (Sharma, 2003;Choiprasert et al, 2011;Tong-un et al, 2010b).…”