Relation between concentrations of free glycerol and triglycerides in human sera.

Stinshoff, K.; Weisshaar, Dana; Staehler, F.; Hesse, Deike; Gruber, Wolfgang; Steier, E

doi:10.1093/clinchem/23.6.1029

Cited by 63 publications

(19 citation statements)

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“…Levels of total cholesterol and HDL-C were measured enzymatically on a Hitachi 911 autoanalyzer (Roche Diagnostics, Basel, Switzerland), and low-density lipoprotein cholesterol (LDL-C) was determined directly (Genzyme, Cambridge, MA, USA). Triglycerides were measured enzymatically with correction for endogenous glycerol [25] using a Hitachi 917 analyzer and reagents and calibrators from Roche Diagnostics. Levels of apolipoproteins B 100 and A-I were measured by an immunoturbidometric technique on the Hitachi 911 analyzer [26].…”

Section: Methodsmentioning

confidence: 99%

Lipid biomarkers, hormone therapy and the risk of venous thromboembolism in women

Everett

Glynn

Buring

et al. 2009

Journal of Thrombosis and Haemostasis

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To cite this article: Everett BM, Glynn RJ, Buring JE, Ridker PM. Lipid biomarkers, hormone therapy and the risk of venous thromboembolism in women. J Thromb Haemost 2009; 7: 588-96.Summary. Background: Published reports of a relationship between lipids and incident venous thromboembolism (VTE) are conflicting. Objectives: To clarify the relationship between lipids and VTE risk in healthy women, including potential effect modification by hormone therapy (HT). Patients/methods: Among 27 081 initially healthy women followed prospectively for incident VTE, we measured a full panel of lipid biomarkers, including total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglycerides and apolipoproteins A-I (apo A-I) and B 100 . Results: During a median follow-up of 11.4 years, VTE occurred in 355 women. We observed no relationship between any of the lipids and VTE risk. However, when unprovoked VTE was considered separately (n = 161), both HDL-C and apo A-I were positively associated with risk. Fully adjusted hazard ratios (HR) and 95% confidence intervals (CI) for extreme tertiles of HDL-C and apo A-I were 1.75 (1.13-2.73) and 1.70 (1.10-2.62), respectively. After stratifying by HT use, this relationship was present only among HT users; the HRs for unprovoked VTE for extreme tertiles of HDL-C and apo A-I were 3.58 (1.69-7.58) and 2.88 (1.29-6.42) among users, but only 0.79 (0.39-1.62) and 0.89 (0.50-1.57) among non-users. The interactions were statistically significant (each P interaction <0.05). Conclusions: We observed little evidence that lipid levels predict risk of incident VTE among non-users of HT. High levels of HDL-C and apo A-I associate with unprovoked VTE risk among HT users. This observation likely reflects prothrombotic effects of HT that are concomitant with HDL-C and apo A-I levels, rather than direct effects of those lipids.

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Section: Methodsmentioning

confidence: 99%

Lipid biomarkers, hormone therapy and the risk of venous thromboembolism in women

Everett

Glynn

Buring

et al. 2009

Journal of Thrombosis and Haemostasis

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“…Serum lipids were determined in duplicates using commercially available colorimetric assays on a semi-automated spectrophotometer, according to manufacturer's guidelines. Triglyceride (Triglycerides TR210, Randox) values were corrected for free glycerol by subtracting 0.11 mmol.L -1 (Stinshoff et al, 1977), according to the manufacturer's guidelines. Intra-assay CV for triglycerides, total cholesterol and HDL were 4.96%, 1.99% and 4.66%, respectively.…”

Section: Lipid Assaysmentioning

confidence: 99%

The effects of Montmorency tart cherry juice supplementation and FATMAX exercise on fat oxidation rates and cardio-metabolic markers in healthy humans

2018

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Montmorency tart cherries (Prunus cerasus L.) are rich in anthocyanins, compounds capable of augmenting fat oxidation and regulating metabolic dysfunction. The present study examined whether Montmorency tart cherry juice (MTCJ) supplementation could augment fat oxidation rates at rest and during FATMAX exercise, thus improve cardio-metabolic health. Eleven, healthy participants consumed MTCJ or placebo (PLA) twice daily, in a randomised, counterbalanced order for 20 days. Participants cycled at FATMAX for 1-h pre-, mid- (10 days) and post-supplementation whilst substrate oxidation rates were measured. Before exercise anthropometrics and resting metabolic rate were measured. Blood pressure, serum triglycerides, cholesterol, HDL, total antioxidant status (TAS) and glucose were measured immediately before and after exercise. No significant differences between conditions or interactions were observed for any functional and blood-based cardio-metabolic markers or fat oxidation during exercise or rest (P > 0.05). Pre-exercise TAS (P = 0.036) and HDL (P = 0.001) were significantly reduced from mid- to post-supplementation with MTCJ only. Twenty days' MTCJ supplementation had no effect on fat oxidation; therefore, it is unnecessary for individuals in this participant cohort to consume MTCJ with exercise to improve cardio-metabolic biomarkers.

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“…Glycerol is typically found in plasma at concentrations ranging from 40 to 370 μmol/litre (3.7 to 34.1 μg/ml) and is limited on the upper end due to the renal threshold for glycerol, with excess glycerol being excreted in urine. [3,4] Fat metabolism is dependent upon duration and intensity of activity with trained athletes exhibiting a larger proportion of energy derived from fatty acid oxidation during exercise, [5] which results in increased plasma glycerol concentrations observed after exercise. [6,7] Therefore, glycerol in circulation can arise from (1) metabolism of intramuscular or liver fat stores and (2) administered glycerol or glycerol containing substances.…”

Section: Introductionmentioning

confidence: 99%

A population study of urine glycerol concentrations in elite athletes competing in North America

Kelly¹,

Madsen²,

Sharpe

et al. 2013

Drug Testing and Analysis

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Glycerol is an endogenous substance that is on the World Anti-Doping Agency's list of prohibited threshold substances due to its potential use as a plasma volume expansion agent. The WADA has set the threshold for urine glycerol, including measurement uncertainty, at 1.3 mg/mL. Glycerol in circulation largely comes from metabolism of triglycerides in order to meet energy requirements and when the renal threshold is eclipsed, glycerol is excreted into urine. In part due to ethnic differences in postprandial triglyceride concentrations, we investigated urine glycerol concentrations in a population of elite athletes competing in North America and compared the results to those of athletes competing in Europe. 959 urine samples from elite athletes competing in North America collected for anti-doping purposes were analyzed for urine glycerol concentrations by a gas chromatography mass-spectrometry method. Samples were divided into groups according to: Timing (in- or out-of-competition), Class (strength, game, or endurance sports) and Gender. 333 (34.7%) samples had undetectable amounts of glycerol (<1 μg/mL). 861 (89.8%) of the samples had glycerol concentrations ≤20 μg/mL. The highest glycerol concentration observed was 652 μg/mL. Analysis of the data finds the effects of each category to be statistically significant. The largest estimate of the 99.9(th) percentile, from the in-competition, female, strength athlete samples, was 1813 μg/mL with a 95% confidence range from 774 to 4251 μg/mL. This suggests a conservative threshold of 4.3 mg/mL, which would result in a reasonable detection window for urine samples collected in-competition for all genders and sport classes.

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Relation between concentrations of free glycerol and triglycerides in human sera.

Cited by 63 publications

References 0 publications

Lipid biomarkers, hormone therapy and the risk of venous thromboembolism in women

Lipid biomarkers, hormone therapy and the risk of venous thromboembolism in women

The effects of Montmorency tart cherry juice supplementation and FATMAX exercise on fat oxidation rates and cardio-metabolic markers in healthy humans

A population study of urine glycerol concentrations in elite athletes competing in North America

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