Relation Between Air-Filled Albumin Microbubble and Red Blood Cell Rheology in the Human Myocardium

Ismail, Suad; Jayaweera, Ananda R.; Camarano, Gustavo; Gimple, Lawrence W.; Powers, Eric R.; Kaul, Sanjiv

doi:10.1161/01.cir.94.3.445

Cited by 51 publications

(24 citation statements)

References 23 publications

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“…These microbubbles are injected intravenously and travel through the circulation with rheological properties similar to erythrocytes, enhancing bloodto-tissue contrast [5]. Current FDA-approved microbubble contrast agents are based on a lipid monolayer or albumin shell encapsulating a high-molecular weight gas [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Kheirolomoom

Dayton

Lum

et al. 2007

Journal of Controlled Release

207

175

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A new acoustically-active delivery vehicle was developed by conjugating liposomes and microbubbles, using the high affinity interaction between avidin and biotin. Binding between microbubbles and liposomes each containing 5% DSPE-PEG2kBiotin was highly dependent on avidin concentration and observed above an avidin concentration of 10 nM. With an optimized avidin and liposome concentration, we measured and calculated as high as 1000 to 10,000 liposomes with average diameters of 200 and 100 nm, respectively, attached to each microbubble. Replacing avidin with neutravidin resulted in 3-fold higher binding, approaching the calculated saturation level. Highspeed photography of this new drug delivery vehicle demonstrated that the liposome-bearing microbubbles oscillate in response to an acoustic pulse similar to microbubble contrast agents. Additionally, microbubbles carrying liposomes could be spatially concentrated on a monolayer of PC-3 cells at the focal point of ultrasound beam. As a result of cell-vehicle contact, the liposomes fused with the cells and internalization of NBD-cholesterol occurred shortly after incubation at 37°C, with internalization of NBD-cholesterol substantially enhanced in the acoustic focus.

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Section: Introductionmentioning

confidence: 99%

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Kheirolomoom

Dayton

Lum

et al. 2007

Journal of Controlled Release

207

175

View full text Add to dashboard Cite

show abstract

“…Microbubble contrast agents are generally between 1 and 8 m in diameter, allowing uninterrupted passage through capillaries. Previous studies have demonstrated that the rheology of microbubble agents is similar to that of erythrocytes (22,23,34) and behave as purely intravascular flow tracers. Unlike iodinated contrast agents and chelates used for MRI, microbubble agents are not filtered by the kidney.…”

mentioning

confidence: 99%

Effects of high-fat diet and losartan on renal cortical blood flow using contrast ultrasound imaging

Declèves

Rychak

Smith

et al. 2013

American Journal of Physiology-Renal Physiology

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Declèves AE, Rychak JJ, Smith DJ, Sharma K. Effects of high-fat diet and losartan on renal cortical blood flow using contrast ultrasound imaging. Am J Physiol Renal Physiol 305: F1343-F1351, 2013. First published September 18, 2013 doi:10.1152/ajprenal.00326.2013.-Obesity-related kidney disease occurs as a result of complex interactions between metabolic and hemodynamic effects. Changes in microvascular perfusion may play a major role in kidney disease; however, these changes are difficult to assess in vivo. Here, we used perfusion ultrasound imaging to evaluate cortical blood flow in a mouse model of high-fat diet-induced kidney disease. C57BL/6J mice were randomized to a standard diet (STD) or a high-fat diet (HFD) for 30 wk and then treated either with losartan or a placebo for an additional 6 wk. Noninvasive ultrasound perfusion imaging of the kidney was performed during infusion of a microbubble contrast agent. Blood flow within the microvasculature of the renal cortex and medulla was derived from imaging data. An increase in the time required to achieve full cortical perfusion was observed for HFD mice relative to STD. This was reversed following treatment with losartan. These data were concurrent with an increased glomerular filtration rate in HFD mice compared with STD-or HFD-losartan-treated mice. Losartan treatment also abrogated fibro-inflammatory disease, assessed by markers at the protein and messenger level. Finally, a reduction in capillary density was found in HFD mice, and this was reversed upon losartan treatment. This suggests that alterations in vascular density may be responsible for the elevated perfusion time observed by imaging. These data demonstrate that ultrasound contrast imaging is a robust and sensitive method for evaluating changes in renal microvascular perfusion and that cortical perfusion time may be a useful parameter for evaluating obesity-related renal disease. ultrasound contrast imaging; obesity-related kidney disease; renal perfusion THE GROWING EPIDEMIC OF OBESITY is a serious health and economic burden throughout the world. The consequence of obesity over time is characterized by insulin resistance, hyperglycemia, atherosclerosis, dyslipidemia, and hypertension. This cluster of risk factors referred to as metabolic syndrome occurs with 50% prevalence in patients 50-to 60-yr-old (4) and contributes to cardiovascular disease. Obesity is a major risk factor for diabetes and hypertension, which together account for ϳ70% of all cases of end-stage renal disease (6). However, there is growing evidence suggesting that obesity by itself may be an independent risk factor for the development of vascular dysfunction and renal disease (21,44,66). Obesity-related kidney disease occurs as a result of complex interactions between metabolic and hemodynamic factors and is characterized by changes of renal perfusion, vascular dysfunction, as well as albuminuria, glomerulosclerosis, and tubulointerstitial fibrosis (20,37,48,50). Alterations in blood flow within the cortex or the medulla ma...

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“…Because the microvascular rheology of the microbubbles used for MCE is similar to that of red blood cells (RBC) [101,102],MCE has been shown to be able to noninvasively and accurately quantify MBF velocity [103].…”

Section: Recent Approaches To the Detection And Quantification Of Cormentioning

confidence: 99%

Coronary Flow Reserve and Myocardial Contrast Echocardiography

Moura¹,

Fernández²,

Zamorano³

2004

Contrast Echocardiography in Clinical Practice

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Although there are several diagnostic techniques available for assessing coronary function, the quantification of coronary flow ( CF) and coronary flow reserve ( CFR) by angiography using an intracoronary Doppler flow wire is usually invasive. Recently, there have been several advances in echocardiography, not only in the direct visualization of the coronary arteries, but also in measuring CF and CFR. In this chapter, the current state of myocardial contrast echocardiography will be revisited, as well as the measurement of CF and CFR by transthoracic echocardiography {TTE) and transesophageal echocardiography (TEE), with special emphasis on recent advancements in ultrasound contrastenhanced agents. CF and CFR: Physiological AspectsCoronary resistance is determined not only by the "tone" of the arteries, but also by the extrinsic compression of the coronary arteries by myocardial muscle during systole. The coronary blood flow has the peculiarity of having a two-phase characteristic pattern and the blood supply to the myocardium takes place fundamentally during diastole (Fig. 7.1). The ratio between diastolic and systolic coronary flow velocity is higher than 1.5 in the left coronary artery and its branches. In the right coronary artery (RCA), this diastolic blood flow is less predominant, probably due to the small systolic resistance in the right coronary side [ 1].The left internal mammary artery (LIMA) without anastomosis has a pattern of systolic flow, the native coronary arteries have a twophase pattern with predominant diastolic flow, and bypass graft with LIMA has a twophase pattern, although the diastolic component is undoubtedly less intense than in the native coronary arteries. The ratio of diastolic and systolic blood flow velocity in coronary bypass grafts of the LIMA is smaller in the proximal portion than in the distal segment [2, 3]. However, the flow in coronary artery bypass grafts of the saphena vein shows a predominant diastolic component along the J. L. Zamorano et al., Contrast Echocardiography in Clinical Practice

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Relation Between Air-Filled Albumin Microbubble and Red Blood Cell Rheology in the Human Myocardium

Cited by 51 publications

References 23 publications

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Effects of high-fat diet and losartan on renal cortical blood flow using contrast ultrasound imaging

Coronary Flow Reserve and Myocardial Contrast Echocardiography

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