Relating the composition and interface interactions in the hard corona of gold nanoparticles to the induced response mechanisms in living cells

Szekeres, Gergő Péter; Werner, Stephan; Spedalieri, Cecilia; Drescher, Daniela; Živanović, Vesna; Montes-Bayón, Marı́a; Bettmer, Jörg; Kneipp, Janina

doi:10.1039/d0nr03581e

Cited by 17 publications

(24 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hard corona is made by a protein fraction strongly bound to the surface, while soft corona is formed by loosely bound proteins, maybe via protein-protein interaction. PC significantly alters the identity of ENPs, including physicochemical properties and the bioidentity of ENPs Liu et al, 2020;Szekeres et al, 2020). First, PC formation alters the size, zeta potential, morphology, and aggregation states of ENPs.…”

Section: Introductionmentioning

confidence: 99%

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

Bai

Wang

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Understanding the basic interactions between engineered nanoparticles (ENPs) and biological systems is essential for evaluating ENPs’ safety and developing better nanomedicine. Profound interactions between ENPs and biomolecules such as proteins are inevitable to occur when ENPs are administered or exposed to biological systems, for example, through intravenous injection, oral, or respiration. As a key component of these interactions, protein corona (PC) is immediately formed surrounding the outlayer of ENPs. PC formation is crucial because it gives ENPs a new biological identity by altering not only the physiochemical properties, but also the biobehaviors of ENPs. In the past two decades, most investigations about PC formation were carried out with in vitro systems which could not represent the true events occurring within in vivo systems. Most recently, studies of in vivo PC formation were reported, and it was found that the protein compositions and structures were very different from those formed in vitro. Herein, we provide an in-time review of the recent investigations of this in vivo PC formation of ENPs. In this review, commonly used characterization methods and compositions of in vivo PC are summarized firstly. Next, we highlight the impacts of the in vivo PC formation on absorption, blood circulation, biodistribution, metabolism, and toxicity of administered ENPs. We also introduce the applications of modulating in vivo PC formation in nanomedicine. We further discuss the challenges and future perspectives.

show abstract

Section: Introductionmentioning

confidence: 99%

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

Bai

Wang

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Cells from the three different cell lines, 3T3, HCT-116, and J774, were incubated with NS25 for three hours. All cell lines have been studied extensively by SERS before using spherical gold nanoparticles of different size [ 31 , 33 , 35 , 52 ]. The mouse fibroblast 3T3 and human epithelial carcinoma HCT-116 cell lines were recently shown to internalize by endocytosis HEPES-synthesized nanostars [ 23 ] that are used here from the surrounding culture media, and the interaction of the nanostructures with the biomolecules of the surrounding intracellular environment could be assessed with SERS spectral analysis.…”

Section: Resultsmentioning

confidence: 99%

“…The data indicate that in all cell lines the nanostars mainly display interactions with proteins, indicating the formation of a protein corona [ 31 , 32 ], and that this protein corona displays a different degree of fragmentation in each cell line. These observations are most probably due to different time frames required for uptake, maturation of endosomes, and fusing with lysosomes in each cell line that would lead to such differences in the fragmentation or denaturation of the proteins interacting with the nanostars.…”

Section: Resultsmentioning

confidence: 99%

“…Apart from size [ 24 ], shape [ 25 , 26 , 27 ], and surface chemistry [ 25 , 28 ] of the pristine nanostructures, their protein corona has an impact on the uptake and processing by animal cells, and therefore on potential applications [ 29 , 30 ]. In the case of plasmonic nanoparticles, SERS has been shown as a very useful tool to probe the biomolecular corona and to characterize it in great detail even inside living cells [ 31 , 32 ]. Specifically, a better understanding is being gained on the surface composition of gold nanostructures in the course of the endolysosomal pathway when they are delivered into the cells through the culture medium [ 32 , 33 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

“…It is known that the intracellular processing of nanomaterials is also strongly determined by the type of cell [ 31 , 33 , 38 ]. In more complex biological systems, other cell types, such as macrophages, serving as the first ‘line of defense’ in vivo, may determine the applicability of the nanostructures, and therefore understanding their response is of great interest in nanotechnological applications [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars

et al. 2021

Self Cite

View full text Add to dashboard Cite

Gold nanostars are a versatile plasmonic nanomaterial with many applications in bioanalysis. Their interactions with animal cells of three different cell lines are studied here at the molecular and ultrastructural level at an early stage of endolysosomal processing. Using the gold nanostars themselves as substrate for surface-enhanced Raman scattering, their protein corona and the molecules in the endolysosomal environment were characterized. Localization, morphology, and size of the nanostar aggregates in the endolysosomal compartment of the cells were probed by cryo soft-X-ray nanotomography. The processing of the nanostars by macrophages of cell line J774 differed greatly from that in the fibroblast cell line 3T3 and in the epithelial cell line HCT-116, and the structure and composition of the biomolecular corona was found to resemble that of spherical gold nanoparticles in the same cells. Data obtained with gold nanostars of varied morphology indicate that the biomolecular interactions at the surface in vivo are influenced by the spike length, with increased interaction with hydrophobic groups of proteins and lipids for longer spike lengths, and independent of the cell line. The results will support optimized nanostar synthesis and delivery for sensing, imaging, and theranostics.

show abstract

Differential Macrophage Responses to Gold Nanostars and Their Implication for Cancer Immunotherapy

Kenry

Eschle

Andreiuk

et al. 2022

Advanced Therapeutics

View full text Add to dashboard Cite

Gold nanostars (AuNSs) are actively explored for a range of theranostic and nanomedicine applications. While their biological applications are widely demonstrated, the fundamental interactions of these anisotropic nanomaterials with innate immune cells and their implication for cancer immunotherapy are less investigated. Specifically, it remains unclear how AuNSs interact with macrophages of different phenotypes and how these interactions influence the behavior of macrophages, especially their phagocytic and cancer modulating activities. Herein, the interactions between AuNSs of different shapes and macrophages of different phenotypes, including the naïve M0, pro‐inflammatory M1‐like, and anti‐inflammatory M2‐like phenotypes, are systematically interrogated. This study reveals that the morphology of AuNSs significantly impacts their interactions with macrophages. Macrophage viability, oxidative stress response, cytokine release, and phagocytotic ability depend on the macrophage phenotype as well as AuNS shape tip‐to‐core ratio. The selective response of macrophages toward AuNSs, particularly those of M1‐like phenotype, may be potentially leveraged for realizing cancer immunotherapy. Overall, it is anticipated that the findings of this study will further shape the understanding of the interactions between innate immune cells and Au nanomaterials and potentially open new opportunities for cancer therapy.

show abstract

Relating the composition and interface interactions in the hard corona of gold nanoparticles to the induced response mechanisms in living cells

Abstract: Understanding the formation of the intracellular protein corona of nanoparticles is essential for a wide range of bio- and nanomedical applications. The innermost layer of the protein corona, the hard...

Cited by 17 publications

References 69 publications

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars

Differential Macrophage Responses to Gold Nanostars and Their Implication for Cancer Immunotherapy

Contact Info

Product

Resources

About