Relating polymeric microparticle formulation to prevalence or distribution of fibronectin and poly-d-lysine to support mesenchymal stem cell growth

Abstract

Protein-coated polymer-based microparticles are attractive supports for cell delivery, but the interplay between microparticle properties, protein coating, and cell response is poorly understood. The interest in alternative microparticle formulations increases the need for a better understanding of how functional protein coatings form on different microparticles. In this work, microparticle formulations based on biodegradable polymers [poly (lactic-co-glycolic acid) (PLGA) and the triblock copolymer PLGA-poloxamer-PLGA] were prepared via an emulsion-based process. To explore the impact that the use of a surfactant has on the properties of the microparticles, the emulsion was stabilized by using either a surfactant, poly(vinyl alcohol), or an organic solvent, propylene glycol. Four different types of microparticles were prepared through combinations of the two types of polymers and the two types of stabilizers. The coating of microparticles with proteins/polypeptides such as fibronectin and poly-d-lysine has been demonstrated before and is an integral step for their application as microcarriers, e.g., for cell delivery; however, the impact of the microparticles’ surface chemical properties on the formation (prevalence and distribution) of the mixed polypeptide coatings and the influence on subsequent cell attachment remain to be elucidated. Using a colocalization analysis approach on ToF-SIMS images of protein-coated microparticles, we show that the use of propyleneglycol over PVA as well as the substitution of PLGA by the triblock copolymer resulted in enhanced protein adsorption. Furthermore, if propyleneglycol is used, the substitution of PLGA with the triblock copolymer leads to increased stem cell adhesion.

Pharmacology active microcarriers delivering HGF associated with extracellular vesicles for myocardial repair

“…Optimization of the cell-carrier is therefore a key element to obtain effective cell adhesion. In this regard, we previously showed that the use of PDL allows interaction with FN, negatively charged, to facilitate its adsorption on the surface of the PAMs [22,23]. Another ECM protein was tested, vitronectin, which despite a higher protein coverage of the surface of PAMs compared to FN resulted in a diminished lEV adhesion (data not shown).…”

Surface Modification Progress for PLGA-Based Cell Scaffolds

“…It must also be noted that the surface properties of PLGA microspheres can impact the effect of the coating. When propylene glycol is used instead of polyvinyl alcohol as the required surfactant during the preparation of PLGA microspheres, the coating distribution of the poly(D-lysine/fibulin) mixture and the subsequent attachment of MSCs are affected [ 98 ].…”

“…Polymers 2024, 16, x FOR PEER REVIEW 9 of 29 instead of polyvinyl alcohol as the required surfactant during the preparation of PLGA microspheres, the coating distribution of the poly(D-lysine/fibulin) mixture and the subsequent attachment of MSCs are affected [98]. Furthermore, a high-molecular-weight (Mw) poly-L-lysine (27.4 kDa, 36.6 kDa) may affect the metabolic activity of cells and it has a specific cytotoxicity [99], which is related to the inhibition of mitochondrial function [100].…”