Relatedness between the Coagulase Gene 3′‐End Region and Coagulase Serotypes among <i>Staphylococcus aureus</i> Strains

Kanemitsu, Keiji; Yamamoto, Hiroyuki; Takemura, Hiromu; Kaku, Mitsuo; Shimada, Jingoro

doi:10.1111/j.1348-0421.2001.tb01270.x

Cited by 21 publications

(17 citation statements)

References 15 publications

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“…The sizes of the products ranged from 350 to 917 bp in increments of 81 bp, reflecting the number of 81-bp repeat units contained in the coa gene after digestion with AluI and this allowed the differentiation of 31 coa-RFLP types and was able to distinguish them from each other confirming the sensitivity of coa/RFLP in differentiation [23]. This difference between our results and other studies [23,28] might be due to our small sized samples or due to differences in MRSA strains as mentioned in previous studies [6,16,31] who reported that S. aureus strains of the same coagulase serotype can be differentiated by Alu restriction and nucleotides sequence analysis of coa gene. In the current study, nucleotides sequence variability of tandem repeat region in coa-gene PCR products was able to discriminate the examined unclassified isolates.…”

Section: Discussionsupporting

confidence: 65%

Phenogenotyping of Closely Related Methicillin Resistant Staphylococcus aureus Isolated from Milk and Meat Products

Ahmad

Ammar

Bendary

et al. 2017

Zagazig Veterinary Journal

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Differentiation of closely related methicillin resistant staphylococcus aureus (MRSA) isolated from food chain is required for epidemiological and food safety studies. This study provided evidence for using coagulase (coa) gene sequence repeats as a powerful diagnostic tool for differentiation of closely MRSA isolated from different sources. Staphylococcal species were isolated from milk, meat, and meat-products. Staphylococcus aureus strains with identical antibiograms were differentiated using enterotoxin genotyping, coa gene polymorphism, the patterns of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of coa and nucleotides sequencing of coa gene-PCR products. Nine CoPs and MRSA strains were closely related and could not be differentiated by antibiogram. Six strains of them were differentiated by enterotoxin genotyping and PCR-RFLP of coa gene and three strains were accurately differentiated by nucleotides sequencing of coa gene-PCR products. In conclusion, the above findings highlight the promising role of coa repeat region nucleotide sequences in differentiation of closely related MRSA in food chain that can help in their diagnosis and epidemiological studies.

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Section: Discussionsupporting

confidence: 65%

Phenogenotyping of Closely Related Methicillin Resistant Staphylococcus aureus Isolated from Milk and Meat Products

Ahmad

Ammar

Bendary

et al. 2017

Zagazig Veterinary Journal

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“…The seven S. aureus strains were as follows: strain 104 (type I), Stp-28 (type IV), no. 55 (type V), Stp-12 (type VI), Ku (type VIII), 17573 (type IX), and 19 (type X) (23,45). To investigate the genetic relatedness between serotypes of coa and genetic backgrounds of S. aureus strains, we selected nine strains whose ribotypes had been determined and sequenced their coa.…”

Section: Methodsmentioning

confidence: 99%

“…The repeat regions comprising 27-amino-acid tandem repeats are highly polymorphic in the number of repeats. Although the serotypes do not correlate with the polymorphism of 27-amino-acid tandem repeats (23), both the N-terminal region and the C-terminal region have been used for S. aureus differentiation by analyzing their diversity using amplified DNA fragments and subsequent restriction fragment length polymorphism analysis.…”

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confidence: 99%

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Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus

et al. 2005

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Staphylocoagulase detection is the hallmark of a Staphylococcus aureus infection. Ten different serotypes of staphylocoagulases have been reported to date. We determined the nucleotide sequences of seven staphylocoagulase genes (coa) and their surrounding regions to compare structures of all 10 staphylocoagulase serotypes, and we inferred their derivations. We found that all staphylocoagulases are comprised of six regions: signal sequence, D1 region, D2 region, central region, repeat region, and C-terminal sequence. Amino acids at both ends, 33 amino acids in the N terminal (the signal sequences and the seven N-terminal amino acids in the D1 region) and 5 amino acids in the C terminal, were exactly identical among the 10 serotypes. The central regions were conserved with identities between 80.6 and 94.1% and similarities between 82.8 and 94.6%. Repeat regions comprising tandem repeats of 27 amino acids with a 92% identity on average were polymorphic in the number of repeats. On the other hand, D1 regions other than the seven N-terminal amino acids and D2 regions were less homologous, with diverged identities from 41.5 to 84.5% and 47.0 to 88.9%, respectively, and similarities from 53.5 to 88.7% and 56.8 to 91.9%, respectively, although the predicted prothrombin-binding sites were conserved among them. In contrast, flanking regions of coa were highly homologous, with nucleotide identities of more than 97.1%. Phylogenetic relations among coa did not correlate with those among the flanking regions or housekeeping genes used for multilocus sequence typing. These data indicate that coa could be transmitted to S. aureus, while the less homologous regions in coa presumed to be responsible for different antigenicities might have evolved independently.

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“…Coagulase typing, i.e., the neutralization of S. aureus coagulation of citrate-plasma with specific antiserum, has been used to distinguish 10 different serological Coa types (17). Coa types have also been analyzed by DNA sequencing, which revealed significant variation within coa sequences for the D12 domain and little variation for the linker and repeat regions, respectively (44).…”

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confidence: 99%

Coagulases as Determinants of Protective Immune Responses against Staphylococcus aureus

et al. 2012

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ABSTRACTDuring infection,Staphylococcus aureussecretes two coagulases (Coa and von Willebrand factor binding protein [vWbp]), which, following an association with host prothrombin and fibrinogen, form fibrin clots and enable the establishment of staphylococcal disease. Within the genomes of differentS. aureusisolates, coagulase gene sequences are variable, and this has been exploited for a classification of types. We show here that antibodies directed against the variable prothrombin binding portion of coagulases confer type-specific immunity through the neutralization ofS. aureusclotting activity and protection from staphylococcal disease in mice. By combining variable portions of coagulases from North American isolates into hybrid Coa and vWbp proteins, a subunit vaccine that provided protection against challenge with different coagulase-typeS. aureusstrains in mice was derived.

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Relatedness between the Coagulase Gene 3′‐End Region and Coagulase Serotypes among Staphylococcus aureus Strains

Cited by 21 publications

References 15 publications

Phenogenotyping of Closely Related Methicillin Resistant Staphylococcus aureus Isolated from Milk and Meat Products

Phenogenotyping of Closely Related Methicillin Resistant Staphylococcus aureus Isolated from Milk and Meat Products

Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus

Coagulases as Determinants of Protective Immune Responses against Staphylococcus aureus

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